基于细胞自噬的维生素C抗黑素生成作用研究

doi:10.3969/j.issn.2097-2806.2025.01.010

Abstract

Abstract:

The aim of this study was to investigate the role of cellular autophagy pathway in the regulation of melanogenesis in vitamin C （VC）-inhibited mouse melanoma cells （B16）. The maximum safe dose of VC on B16 cells was detected by MTT assay, the melanogenesis was induced in B16 cells by adding α-melanotropic hormone （α-MSH）, and then the mechanism of cellular autophagy in the anti-melanogenic effect of VC was investigated by using an autophagy inhibitor （3-MA）. The results show that VC at 500 μg/mL and below have no effect on the survival of B16 cells. VC at 20 μg/mL and 50 μg/mL significantly inhibit α-MSH-induced melanin synthesis in B16 cells （p<0.01）. Compared with the blank group, VC significantly induces the expression of autophagy-related proteins LC3-Ⅱ and p62 in B16 cells, and the expression of mTOR protein decreases （p<0.05）. To further investigate the effect of autophagy on melanogenesis, B16 cells are pretreated with the autophagy inhibitor 3-MA, and it is found that 3-MA abolishes VC-induced autophagy up-regulation, as well as the anti-melanogenesis effect in B16 cells. These results suggest that VC can inhibit melanogenesis by activating the autophagy system in B16 melanoma cells.

Key words: vitamin C, melanin, autophagy, in vitro assay

CLC Number:

TQ658

Fan Zhang, Dongqin He, Qianjin Bian, Fan Wu, Yi Qin, Yao Pan. Study on the anti-melanogenesis effect of vitamin C based on cellular autophagy[J].China Surfactant Detergent & Cosmetics, 2025, 55(1): 83-88.

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References 15

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Study on the anti-melanogenesis effect of vitamin C based on cellular autophagy

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