酵母菌/锌发酵产物在头皮护理中的功效研究

doi:10.3969/j.issn.2097-2806.2024.09.011

Abstract

Abstract:

The efficacy of yeast/zinc fermentation products in scalp care were studied from the aspects of safety, oil control and dandruff removal, and hair follicle maintenance. The safety of yeast/zinc fermentation products was tested through human patch testing. In vitro SZ95 cell model was used to study the oil-control efficacy of yeast/zinc fermentation products. The method of qRT-PCR was used to verify the expression of oil-secretion-related genes SREBP-1c, PPARG, and PI3K. Spectrophotometry was used to test the effect of yeast/zinc fermentation products on 5α-reductase. The oil-control effect of yeast/zinc fermentation products on scalp and the maintenance effect on hair follicles were studied through human efficacy testing and in vitro hair follicle model, respectively. The results showed that the yeast/zinc fermentation products at a content of 5% was safe and harmless to human health; the yeast/zinc fermentation products with a content of 0.8%-1.5% could significantly inhibit the activity of 5α-reductase. The yeast/zinc fermentation products at a content of 0.5%-2.0% could significantly inhibit the secretion of oil in SZ95 cells in vitro, and significantly downregulate the expression of oil-secretion-related genes SREBP-1c and PPARG. The yeast/zinc fermentation products at content of 1.0% and 2.0% could significantly inhibit the expression of PI3K. The yeast/zinc fermentation products at a content of 5% could significantly reduce scalp oil secretion, improve hair follicle cycle, and promote hair shaft growth. Therefore, the yeast/zinc fermentation products had certain scalp care effects and had positive effects in controlling oil, improving hair follicle health, and promoting hair shaft growth.

Key words: yeast/zinc fermentation product, oil control, anti-dandruff, efficacy evaluation

CLC Number:

TQ658

Xiaopeng You, Ning Peng, Zhixian Chen. Study on the efficacy of yeast/zinc fermentation products in scalp care[J].China Surfactant Detergent & Cosmetics, 2024, 54(9): 1099-1105.

Figures/Tables 12

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References 15

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基因名称	引物序列
GAPDH	F: AATGAATGGGCAGCCGTTAG
GAPDH	R: AGAGTTAAAAGCAGCCCTGG
SPEBP-1c	F: CAAAGGAGCTGATGGATGTGC
SPEBP-1c	R: AAACAAACATCGGGAAGAGCTA
PPARG	F: GCATTTCTGCATTCTGCTTA
PPARG	R: GTGTCAACCATGGTCATTTC
PI3K	F: TCCCATAGTCATGCATTGTT
PI3K	G: GAATGGAAGACGGGAGATTC

试验分组	样品信息		样本量	检测系统
试验分组	刺激条件	样品浓度	每组毛囊数	检测模型	检测指标
BC			6	人离体毛囊	1．毛囊所处周期占比； 2．毛囊平均增长长度
NC	200 μmol/L H₂O₂
PC		胰岛素10 μg/mL，氢化可的松10 ng/mL
酵母菌/锌发酵产物		5%

反应程度	评分等级	皮肤反应
-	0	阴性反应
±	1	可疑反应，仅有微弱红斑
+	2	弱阳性反应（红斑反应）；红斑、浸润、水肿、可有丘疹
++	3	强阳性反应（疱疹反应）；红斑、浸润、水肿、丘疹、疱疹；反应可超出受试区
+++	4	极强阳性反应（融合性疱疹反应）；明显红斑、严重浸润、水肿、融合性疱疹、反应超出受试区

Study on the efficacy of yeast/zinc fermentation products in scalp care

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组别	受试人数	观察时间/h	斑贴试验不同皮肤反应人数
组别	受试人数	观察时间/h	0	1	2	3	4
受试物	30	0.5	30	0	0	0	0
		24	27	3	0	0	0
		48	28	2	0	0	0
对照	30	0.5	30	0	0	0	0
		24	30	0	0	0	0
		48	30	0	0	0	0

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