液质联用技术在化妆品抗感染类药物检测中的应用

doi:10.3969/j.issn.2097-2806.2023.12.012

Abstract

Abstract:

The effects and safety risks of adding anti-infective drugs in cosmetics were summarized. The common types, characteristics and application fields of liquid chromatography-mass spectrometry were introduced. The methods in “Safety and Technical Standards for Cosmetics （2015 Edition）” for determination of anti-infective drugs were also listed in this paper. The advantages of various detection methods were introduced, including liquid chromatography triple quadrupole tandem mass spectrometry （LC-MS/MS）, direct real-time analysis-high resolution mass spectrometry （DART-Q-Exactive-Orbitrap-MS）, ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry （UPLC-Q-Orbitrap MS）, ultra high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry （UPLC-LTQ/orbitrap MS） and ultra high performance liquid chromatography-quadrupole-time-of-flight high resolution mass spectrometry （UPLC-Q-TOF-MS）. In the sample screening process, some scholars detected three compounds that had not yet been detected in the “Safety and Technical Standards for Cosmetics （2015 Edition）”, namely danofloxacin, trimethoprim and ronidazole. This paper can provide data for the relevant scientific research work in cosmetics. At the same time, the risk monitoring work of cosmetics in relevant testing institution will have direction and ideas after understanding the above content. In addition, the exploratory research on the correction of detection method will be more efficient and scientific. Finally, the trend of using liquid chromatography-mass spectrometry to establish methods for determination of anti-infective drugs in cosmetics is recommended. Liquid chromatography-mass spectrometry is still the preferred method for the determination of anti-infective drugs in cosmetics in the future. Above all, high resolution mass spectrometry will be more widely used in the confirmation of known substances and screening of unknown substances.

Key words: liquid chromatography-mass spectrometry, cosmetics, anti-infective drugs, detection method

CLC Number:

TQ658

Bei Hu, Lixia Li, Xiaoping Ding, Wen Lv, Xiaojian Li, Qiong Li. Application of liquid chromatography-mass spectrometry in the detection of anti-infective drugs in cosmetics[J].China Surfactant Detergent & Cosmetics, 2023, 53(12): 1443-1450.

Figures/Tables 4

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Tab. 3

Tab. 4

References 42

[1]	Qi Shangzhong. Research progress on detection methods of hormones and antibiotics in cosmetics[J]. China Cleaning Industry, 2014, 6: 74-77.
[2]	Chen Jing, Zheng Rong, Mao Beiping, et al. Determination of nineteen quinolone antibiotics in cosmetics by LC-MS/MS[J]. Flavour Fragrance Cosmetics, 2020, 6: 80-85, 90.
[3]	Cai Yongtong, Xun Zhiqing, Li Xiuying, et al. Simultaneous determination of four imidazole-based antifungal agents in cosmetics by UPLC-MS/MS and QuEChERS[J]. Flavour Fragrance Cosmetics, 2017, 2: 55-58.
[4]	Wang Tianhao. Research progress of antibiotic detection technology in cosmetics[J]. Yunnan Chemical Technology, 2021, 48 (9) : 13-14, 17.
[5]	Wei Hualin, Zhou Pu, Jiang Pei, et al. Determination of seven antifungal drugs in cosmetics by high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Health Laboratory Technology, 2018, 28 (16) : 1955-1958.
[6]	National Medical Products Administration. Safety and Technical Standards for Cosmetics: 2015 Editon[S]. Beijing: Standards Press of China, 2016.
[7]	Chen Yuwan, Zhou Wen, Li Xinwei, et al. Research progress of protein-protein interaction based on liquid chromatography mass spectrometry[J]. Acta Chimica Sinica, 2022, 80: 817-826. doi: 10.6023/A22010055
[8]	Cao Houhua, Yu Zhongyi. Research progress on correlation between metabonomics and TCM syndrome and objective evaluation of HPLC-MS[J]. Academic Journal of Shanghai University of Traditional Chinese Medicine, 2016, 30 (6) : 83-90.
[9]	Zhu F, Wu X S, Li F, et al. Simultaneous determination of 12 antibacterial drugs in cream disinfection products with EMR-lipid cleanup using ultra-high-performance liquid chromatography tandem mass spectrometry[J]. Analytical Methods, 2019, 11 (32) : 4084-4092. doi: 10.1039/C9AY00937J
[10]	Zhang Lu, Li Keqiang, Zhu Hui, et al. Research progress on illegal additions of chemical drugs in health food for weight loss and their detection technologies[J]. Journal of Food Safety and Quality, 2021, 12 (3) : 904-913.
[11]	Soares K L, Caldú A S, Barbosa S C, et al. Rapid and cost-effective multiresidue analysis of pharmaceuticals, personal care products, and antifouling booster biocides in marine sediments using matrix solid phase dispersion[J]. Chemosphere, 2021, 267: 129085. doi: 10.1016/j.chemosphere.2020.129085
[12]	Papageorgiou M, Zioris I, Danis T, et al. Comprehensive investigation of a wide range of pharmaceuticals and personal care products in urban and hospital wastewaters in Greece[J]. Science of the Total Environment, 2019, 694: 133565. doi: 10.1016/j.scitotenv.2019.07.371
[13]	Yang Chenye, Xu Shiwei, Han Wei, et al. Analysis of macrolide antibiotics residues in animal derived food by liquid chromatography-mass spectrometry: a review[J]. Journal of Changzhou University (Natural Science Edition), 2017, 29 (5) : 85-92.
[14]	Zhan J, Ni M L, Zhao H Y, et al. Multiresidue analysis of 59 nonallowed substances and other contaminants in cosmetics[J]. Separation Science, 2014, 37 (24) : 3684-3690.
[15]	Tan Juying, Yan Qishuang, Wang Juan, et al. Application of liquid chromatography-mass spectrometry in the analysis of prohibited substances in cosmetics[J]. China Surfactant Detergent & Cosmetics, 2018, 48 (9) : 527-533, 540.
[16]	Wu C S, Zhang J L, Qiao Y L, et al. Simultaneous determination of 14 β-lactam antibiotics in cosmetic products by liquid chromatography tandem mass spectrometry method[J]. Chinese Chemical Letters, 2011, 22: 334-337. doi: 10.1016/j.cclet.2010.09.035
[17]	Sha Lina, Yang Guangyong, Mu Xiaojuan, et al. Determination of 16 kinds of anti-infective drugs in cosmetics by LC-MS/MS[J]. China Surfactant Detergent & Cosmetics, 2021, 51 (8) : 802-808.
[18]	Lu Linling, Qian Yefei, Zhang Bin, et al. Determination of 38 anti-infective drugs in cosmetics by ultra high performance liquid chromatography-tandem mass spectrometry[J]. China Surfactant Detergent & Cosmetics, 2022, 52 (2) : 214-223.
[19]	Wang Qingling, Qiu Juntao, Zhan Yuecheng, et al. A quick determination of 36 kinds of forbidden anti- infective drugs residue in cosmetics by ultra performance liquid chromatograph-tandem mass spectrometry combined with the dSPE[J]. Farm Products Processing, 2021, 9: 43-47, 53.
[20]	Wang Xin, Chen Rong. Simultaneous detection of 41 forbidden anti-infective drugs in cream cosmetics by LC-MS /MS[J]. China Pharmacist, 2018, 21 (8) : 1388-1392.
[21]	Wang Ning, Zhang Huansheng, Sui Yujie, et al. Determine 8 kinds of macrolides and lincomycin antibiotics in cosmetics by UPLC-MS/MS[J]. China Cosmetics Review, 2021, 2: 112-115.
[22]	Ye Zhuhong, Dai Dan, Zhu Dandan, et al. Determination of lincomycin and clindamycin in cosmetics by HPLC-MS/MS[J]. Flavour Fragrange Cosmetics, 2021, 4: 76-80.
[23]	Yang Na, Ye Leihai, Ye Jiaming, et al. Simultaneous determination of 20 antibiotic drugs in cosmetices by high performance liquid chromatography-tandem mass spectrometry method of content study[J]. Farm Products Processing, 2019, 4: 50-53.
[24]	Lu Jian, Xun Zhiqing, Wang Chenxia, et al. Determination of 15 quinolone antibiotics in cosmetics by liquid chromatography tandem mass spectrometry[J]. China Surfactant Detergent & Cosmetics, 2019, 49 (6) : 403-409.
[25]	Sun Shanshan, Yang Yang, Ma Ming, et al. Determination of nine kinds of prohibited penicillin in cosmetic by HPLC-MS/MS[J]. Flavour Fragrance Cosmetics, 2019, 5: 26-29.
[26]	Yao Wei, Sun Xuemei, Yang Minghua. Determination of quinolones in cosmetics by liquid chromatograhy- tandem mass spectrometry[J]. Yunnan Chemical Technology, 2019, 46 (3) : 101-103.
[27]	Zhang Yanhui, Li Lingling, Wang Hongmei. Simultaneous determination of 10 antibiotics in cosmetics by liquid chromatography tandem mass spectrometry[J]. Chinese Journal of Health Laboratory Technology, 2017, 27 (18) : 2600-2602.
[28]	Xiao Ruina, Li Yuli. Simultaneous determination of 40 antibacterial agents in cosmetics by liquid chromatography tandem mass spectrometry[J]. China Surfactant Detergent & Cosmetics, 2020, 50 (6) : 419-426.
[29]	Fan Fei, Zheng Yan, Liu Pengming, et al. Simultaneous measurement of eight antifungal drugs in cosmetics by UPLC-MS/MS[J]. Flavour Fragrance Cosmetics, 2022, 1: 55-57.
[30]	Zhang Junfang. Rapid determination of antibacterial agents in skin care hyaluronic acid by high performance liquid chromatography-tandem mass spectrometry[J]. China Surfactant Detergent & Cosmetics, 2022, 52 (6) : 677-682.
[31]	Lan Cao, Shao Linzhi, Chen Simin. Rapid determination of chloramphenicol in cosmetics using direct real-time analysis-high resolution mass spectrometry[J]. Journal of Instrumental Analysis, 2019, 38 (12) : 1503-1506.
[32]	Liao Huayong, Wang Jing, Li Hongyu. Rapid determination of 17 quinolones in cosmetics using ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry[J]. Journal of Instrumental Analysis, 2019, 38 (9) : 1102-1107.
[33]	Niu Zengyuan, Luo Xin, Wang Fengmei, et al. Fast screening and confirmation of 89 prohibited compounds in cosmetics using UPLC-LTQ/Orbitrap MS[J]. Journal of Chinese Mass Spectrometry Society, 2016, 37 (3) : 201-212. doi: 10.7538/zpxb.youxian.2015.0051
[34]	Li Hongying, Shen Huadan, Fang Jiangji, et al. Rapid determination of 66 antibiotics in cosmetics using ultra high performance liquid chromatography-linear ion trap/orbitrap mass spectrometry[J]. Chinese Journal of Chromatography, 2018, 36 (7) : 643-650. doi: 10.3724/SP.J.1123.2018.01041
[35]	Wang Fengmei, Niu Zengyuan, Luo Xini, et al. Screening and confirmation of antibiotics in cosmetics by high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry[J]. Journal of Food Safety and Quality, 2014, 5 (12) : 3911-3921.
[36]	Zhang Dandan, Ouyang Hui. Progress in qualitative and quantitative analysis of saponins by LC-MS[J]. Progress in Pharmaceutical Sciences, 2017, 41 (8) : 608-613.
[37]	Dong Yalei, Niu Shuijiao, Qiao Yashen, et al. Determination of 19 illegally added chemical ingredients in hair loss prevention cosmetics by ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry[J]. Chinese Journal of Chromatography, 2022, 40 (4) : 343-353. doi: 10.3724/SP.J.1123.2021.08019
[38]	Li Yangjie, Huang Jiaying, Fang Jihui, et al. Fast screening and confirmation of 73 common prohibited compounds in cosmetics by ultra performance liquid chromatography-quadrupole-time-of-flight high resolution mass spectrometry[J]. Chinese Journal of Chromatography, 2022, 40 (5) : 434-442.
[39]	Zheng Jiaxin, Jia Changping. Determination of 23 illegally added antibiotics by UPLC-ESI-Q-TOF/MS in cosmetics[J]. China Surfactant Detergent & Cosmetics, 2021, 51 (12) : 1250-1258.
[40]	Wang Ling, Wang Xinmei, Zhao Zeyuan, et al. Understand the essence of cosmetic quality through date[J]. China Cosmetics Review, 2021 (Z1) : 14-19.
[41]	Liu Meiyun, Dong Qing, Li Zhu, et al. Analysis of the results of cosmetics sampling and testing in various provinces, municipalities and autonomous regions in China from 2017 to 2019[J]. Guangdong Chemical Industry, 2021, 48 (2) : 71-73.
[42]	Zhang Xinxin, Gao Yuanhui, Hua Jin, et al. Determination of five kinds of nitroimidazole compound in whelk dispelling cosmetics by liquid chromatography tandem mass spectrometry[J]. China Surfactant Detergent & Cosmetics, 2016, 46 (11) : 661-664, 670.

方法编号	分析仪器	检测的抗感染类药物
2.1	液相色谱-三重四极杆质谱联用仪	氟康唑、酮康唑、萘替芬、联苯苄唑、克霉唑、益康唑、灰黄霉素、咪康唑和环吡酮胺
2.2	高效液相色谱仪	盐酸美满霉素、甲硝唑、二水土霉素、盐酸四环素、盐酸金霉素、盐酸多西环素和氯霉素
2.3	液相色谱-三重四极杆质谱联用仪	依诺沙星、氟罗沙星、氧氟沙星、诺氟沙星、培氟沙星、环丙沙星、恩诺沙星、沙拉沙星、双氟沙星和莫西沙星
2.35	液相色谱-三重四极杆质谱联用仪	甲硝唑、磺胺吡啶、磺胺甲嘧啶、磺胺甲二唑、磺胺甲氧嗪、磺胺氯哒嗪、磺胺甲噁唑、依诺沙星、沙拉沙星、培氟沙星、氧氟沙星、氟罗沙星、双氟沙星、莫西沙星、诺氟沙星、环丙沙星、恩诺沙星、呋喃它酮、林可霉素、克林霉素、克林霉素磷酸酯、四环素、多西环素、米诺环素、土霉素、金霉素、阿奇霉素、克拉霉素、罗红霉素、氯霉素、螺内酯、灰黄霉素、克霉唑、酮康唑、氟康唑和联苯苄唑

检测种数	色谱柱	质谱模式	检出限/（μg/g）	定量限/（μg/g）	回收率/%	文献
19种	Agilent SB C₁₈ （2.1 mm×100 mm, 1.7 μm）	ESI⁺	0.001	—	85~115	[2]
4种	Welch Ultimate XB C₁₈ （4.6 mm×150 mm, 5 μm）	ESI⁺	—	0.005	82.5~104.4	[3]
7种	BEH C₁₈ （50 mm×2.1 mm, 1.7 μm）	ESI⁺	0.02~0.50	—	88.0~97.2	[5]
14种	Agilent Zorbax SB-C₁₈ （100 mm×2.1 mm, 3.5 μm）	ESI⁺	<0.008	—	87.5~115.5	[16]
16种	Waters ACQUITY BEH C₁₈ （100 mm×2.1 mm, 1.7 μm）	ESI⁺	0.05~0.50	0.25~2.50	86.0~112.4	[17]
38种	Waters ACQUITY BEH C₁₈ （50 mm×2.1 mm, 1.7 μm）	ESI⁺，ESI^-	0.000 3~0.24	0.001 1~0.80	79.0~119.8	[18]
36种	Agilent SB-C₁₈ （2.1 mm×100 mm, 1.8 μm）	ESI⁺，ESI^-	0.20~0.50	—	85~110	[19]
41种	Waters ACQUITY BEH C₁₈ （2.1 mm×100 mm, 1.7 μm）	ESI⁺，ESI^-	0.01~0.50	—	72.3~112.5	[20]
8种	Zorbax Eclipse Plus C₁₈ （3.0 mm×100 mm, 3.5 μm）	ESI⁺	0.001~0.02	—	80.0~106.9	[21]
2种	CNW Athena C₁₈-WP （100 mm×2.1 mm, 3 μm）	ESI⁺	0.10	0.30	86.1~ 98.6	[22]
20种	Agilent EC-C₁₈（Poroshell 120）（3.0 mm×50 mm, 2.7 μm）	ESI⁺	0.01~0.50	0.05~1.00	71.5~117.6	[23]
15种	Thermo Hypersil GOLD C₁₈ （2.1 mm×100 mm, 1.9 μm）	ESI⁺	0.15	0.50	80.1~104.9	[24]
9种	Agilent Zorbax SB-C₁₈ （150 mm×2.1 mm, 3.5 μm）	ESI⁺	0.010	0.030	83.1~110.5	[25]
10种	Agilent Zorbax SB-C₁₈ （2.1 mm×100 mm, 1.8 μm）	ESI⁺	0.1~0.2	—	84.3~99.2	[26]
10种	Agilent Zorbax SB-C₁₈ （4.6 mm×75 mm, 3.5 μm）	ESI⁺	0.01~0.04	0.02~0.1	76.8~96.9	[27]
40种	Luna C₁₈ （150 mm × 4.6 mm, 3 μm）	ESI⁺	0.025~0.25	0.075~0.75	73.8~109.1	[28]
8种	Agilent Zorbax SB-C₈ （100 mm × 2.1 mm, 3.5 μm）	ESI⁺	1~95	5~475	91.71~101.66	[29]
20种	Merck ZIC^?-HILIC C₁₈ （50 mm × 4.6 mm, 5 μm）	ESI⁺	0.9~201	5~401	—	[30]

检测种数	提取净化方法	色谱柱	流动相及流速	质谱模式	检出限/（μg/kg）	定量限/（μg/kg）	回收率/%	文献
37种	甲醇超声提取	Waters ACQUITY BEH C₁₈（2.1 mm×100 mm, 1.7 μm）	正离子模式：含0.1%甲酸的5 mmol/L乙酸铵溶液-乙腈梯度洗脱；负离子模式：5 mmol/L乙酸铵溶液-乙腈梯度洗脱；流速均为0.25 mL/min	ESI⁺，ESI^-	—	5~10	60~117	[33]
66种	乙腈超声提取	UPLC C₁₈（100 mm×2.1 mm, 1.8 μm）	0.1%甲酸水溶液-乙腈梯度洗脱，0.2 mL/min	ESI⁺	2~4	5~10	58.2~119.1	[34]
36种	甲醇超声提取，正己烷除脂	Waters ACQUITY BEH C₁₈（2.1 mm×100 mm, 1.7 μm）	含5 mmol/L乙酸铵和0.1%甲酸的水溶液-乙腈梯度洗脱，0.25 mL/min	ESI⁺	5~10	—	54.15~117.55	[35]

检测种数	提取净化方法	色谱柱	流动相及流速	质谱模式	检出限/（μg/g）	定量限/（μg/g）	回收率/%	文献
8种	甲醇超声提取	Waters ACQUITY BEH C₁₈（100 mm× 2.1 mm, 1.7 μm）	乙腈-含0.05%甲酸的2 mmol/L 甲酸铵水溶液梯度洗脱，0.35 mL/min	ESI⁺	0.025~0.05	0.075~0.15	68.6~118.8	[37]
25种	饱和氯化钠分散，0.2%甲酸乙腈超声提取，PSA净化	Waters ACQUITY HSS T₃（100 mm× 2.1 mm, 1.8 μm）	正离子模式：含0.1%甲酸的5 mmol/L乙酸铵溶液-含0.1%甲酸的甲醇梯度洗脱；负离子模式：0.005%甲酸水溶液-含0.005%甲酸的甲醇梯度洗脱；流速均为0.35 mL/min	ESI⁺，ESI^-	0.005~0.15	0.015~0.45	60.3~130.3	[38]
23种	乙腈超声提取	Waters ACQUITY HSS T₃（100 mm× 2.1 mm, 1.8 μm）	0.1%甲酸溶液-0.1%甲酸乙腈梯度洗脱，0.3 mL/min	ESI⁺	0.05~1.36	0.16~4.53	74.1~125.5	[39]

Application of liquid chromatography-mass spectrometry in the detection of anti-infective drugs in cosmetics

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