Abstract:

Herbal extracts which are safer and more acceptable compared with chemical compounds and hormone products, may contain agonists in the Nrf-2/HO-1 pathway that mobilize the body to produce effective endogenous antioxidant capacity delaying aging and reducing elastase content to keep skin elastic and firm. Antioxidant capacity and elastase inhibition rate of P. emblica extract were tested to obtain its effects on anti-aging. Under the action of 5α-reductase, testosterone is reduced to DHT, whereas high content of DHT is the primary cause of the AGA. Inhibiting 5α-reductase by plant extracts to reduce DHT content is an effective way to alleviate AGA. DP is the signal center of hair growth and the target of testosterone in the hair follicle which expresses rich 5α-reductase. In vitro 3D cultivation of HFDPC, an ideal detection model, can not only agglomerate them into balls to resemble growth environment in vivo, but also maintain hair induction ability. The 5α-reductase inhibiting ability, the cell viability of HFDPC after 3D cultivation and in vitro human hair growth rate were examined to acquire its effects on hair growth promotion. The results suggest that P. emblica extract is supposed to delay senescence through inhibition of oxidization and elastase activity, and to facilitate hair growth through inhibition of 5α-reductase and β-galactosidase, enhancement of HFDPC proliferation and inductivity, and promotion of in vitro human hair follicle growth. We evaluate the anti-aging and hair growth promotion effect of the P. emblica to provide reference for further researches.

Key words: P. emblica extract, anti-aging, 3D cultivation of HFDPC, in vitro human hair follicle, hair growth promotion