光甘草定的美白作用及其机制

doi:10.3969/j.issn.2097-2806.2026.05.010

Abstract

Abstract:

This study aimed to investigate the whitening effect of glabridin （GLA） and its mechanism. A mouse model of ultraviolet B （UVB） -induced skin photoaging （SP） mice was used. The mice were randomly assigned to 6 treatment groups （n=12）, which were control group （C group）, UVB group, low, medium and high dose glabridin group （L, M, H-GLA）, and high dose glabridin + nuclear factor E2-related factor 2 （Nrf2） inhibitor ML385 group （H-GLA+ML385）. Group C was conventionally fed without UVB irradiation, while the other groups were subjected to UVB irradiation. Each group received percutaneous administration 1 h before UV exposure once daily for 8 weeks. The skin was analyzed by hematoxylin and eosin （HE） staining and Masson’s trichrome staining. Melanin content was determined by the NaOH dissolution method. Tyrosinase activity was detected by ELISA. The activities of superoxide dismutase （SOD）, catalase （CAT）, and glutathione peroxidase （GSH-Px） and the content of malondialdehyde （MDA） were determined using commercial kits. Reactive oxygen species （ROS） were detected by dihydroethidium （DHE） staining. The levels of interleukin-6, tumor necrosis factor-α （TNF-α） and IL-1β in skin tissue were detected by ELISA. mRNA levels of type I collagen α1 chain （COL1A1）, matrix metalloproteinase-1 （MMP-1）, tyrosinase-associated protein （TRP） -1, TRP-2, heme oxygenase-1 （HO-1） and NAD （P） H: quinone oxidoreductase-1 （NQO-1） were detected by RT-qPCT. Western blot was used to analyze protein expression of α-MSH, Nrf2 （nuclear） and Keap1 in the skin. The results show that compared with the UVB group, the three doses of glabridin （L, M and H-GLA groups） alleviate UVB-induced skin damage and collagen degeneration in the skin photoaging mice, decrease the level of MMP-1 mRNA and increase the level of COL1A1 mRNA. decrease skin melanin content, tyrosinase activity, the levels of TRP-1 and TRP-2 mRNA, the relative expression level of α-MSH protein, increase the skin SOD, CAT and GSH-Px activities, decrease MDA content and ROS relative fluorescence intensity, and decrease skin IL-6, TNF-α and IL-1β contents, increase the expression level of Nrf2 （nuclear） protein, and HO-1 and NQO-1 mRNA, and decrease the relative expression level of Keap1 protein （P<0.05）. However, the Nrf2 inhibitor ML385 attenuates the above effects of glabridin （P<0.05）. These results indicate that glabridin alleviates UVB-induced skin damage and collagen degeneration, inhibits melanin production, and reduces oxidative stress and inflammation in mice with skin photoaging by activating Nrf2.

Key words: glabridin, skin whitening, skin photoaging, melanin production, oxidative stress, inflammation

CLC Number:

TQ658

Jun Liang, Xiaoming Shi. Whitening effect of glabridin and its mechanism[J].China Surfactant Detergent & Cosmetics, 2026, 56(5): 635-643.

Figures/Tables 10

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References 29

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基因	序列（5'-3'）
I型胶原α1链（COL1A1）	F: 5′-GCACGAGTCACACCGGAACT-3′
I型胶原α1链（COL1A1）	R: 5′-AAGGGAGCCACATCGATGAT-3′
基质金属蛋白酶-1 （MMP-1）	F: 5′-TTGCCCAGAGAAAAGCTTCAG-3′
基质金属蛋白酶-1 （MMP-1）	R: 5′-TAGCAGCCCAGAGAAGCAACA-3′
酪氨酸酶相关蛋白（TRP） -1	F: 5′-GCTGCGTTGTTACTTGTA-3′
酪氨酸酶相关蛋白（TRP） -1	R: 5′-CTTCATTCTTGGTGCTTCT-3′
TRP-2	F: 5′-TACAATTACGCCGTTGAT-3′
TRP-2	R: 5′-GATTCCAATGACCACTGA-3′
血红素加氧酶-1 （HO-1）	F: 5′-CAAGCCGAGAATGCTGAGTTCATG-3′
血红素加氧酶-1 （HO-1）	R: 5′-GCAAGGGATGATTTCCTGCCAG-3′
NAD （P） H：醌氧化还原酶 -1 （NQO-1）	F: 5′-AGGATGGGAGGTACTCGAATC-3′
NAD （P） H：醌氧化还原酶 -1 （NQO-1）	R: 5′-TGCTAGAGATGACTCGGAAGG-3′
β-actin	F: 5′-TATGGAATCCTGTGGCATC-3′
β-actin	R: 5′-GTGTTGGCATAGAGGTCTT-3′

组别	COL1A1 mRNA相对水平	MMP-1 mRNA相对水平
C	1.00±0.06	1.00±0.07
UVB	0.20±0.02^*	7.92±1.15^*
L-GLA	0.40±0.07^*#	5.07±0.75^*#
M-GLA	0.55±0.06^*#	2.76±0.34^*#
H-GLA	0.80±0.09^*#	1.86±0.26^*#
H-GLA+ML385	0.40±0.07^*#&	5.04±0.79^*#&
F值	244.913	175.106
P值	<0.001	<0.001

组别	黑色素含量/（mg/g）	酪氨酸酶活性/（U/ （mg prot））	TRP-1 mRNA相对表达量	TRP-2 mRNA相对表达量
C	6.14±0.42	2.04±0.21	1.00±0.06	1.00±0.05
UVB	10.94±1.24^*	6.62±0.52^*	5.28±0.58^*	6.04±0.69^*
L-GLA	8.79±0.99^*#	5.05±0.54^*#	3.40±0.31^*#	4.08±0.27^*#
M-GLA	7.79±0.81^*#	3.99±0.58^*#	2.46±0.22^*#	2.95±0.34^*#
H-GLA	6.87±0.63^*#	2.99±0.24^*#	1.52±0.13^*#	1.99±0.11^*#
H-GLA+ML385	8.89±0.96^*#&	5.13±0.59^*#&	3.43±0.28^*#&	4.20±0.32^*#&
F值	44.525	144.383	296.909	293.647
P值	<0.001	<0.001	<0.001	<0.001

组别	SOD含量/（U/（mg prot））	CAT含量/（U/（mg prot））	GSH-Px含量/（U/（mg prot））	MDA含量/（nmol/（mg prot））
C	3.69±0.46	4.41±0.38	142.85±17.58	1.30±0.18
UVB	2.07±0.36^*	1.45±0.09^*	93.42±10.18^*	3.61±0.49^*
L-GLA	2.72±0.40^*#	2.29±0.18^*#	124.38±11.66^*#	2.97±0.35^*#
M-GLA	2.98±0.37^*#	2.83±0.47^*#	128.97±22.67^*#	2.31±0.21^*#
H-GLA	3.47±0.49^*#	3.45±0.37^*#	136.72±12.94^*#	1.80±0.26^*#
H-GLA+ML385	2.73±0.40^*#&	2.35±0.20^*#&	122.21±13.23^*#&	2.94±0.39^*#&
F值	23.488	131.393	15.139	77.853
P值	<0.001	<0.001	<0.001	<0.001

组别	IL-6含量 / （pg / （mg prot））	TNF-α含量 / （pg / （mg prot））	IL-1β含量 / （pg / （mg prot））
C	9.79±1.06	14.67±1.34	5.14±0.45
UVB	32.84±1.33^*	53.84±2.63^*	31.89±3.43^*
L-GLA	23.10±2.11^*#	38.74±1.60^*#	22.46±2.24^*#
M-GLA	19.13±1.41^*#	30.01±1.65^*#	15.56±1.06^*#
H-GLA	14.49±1.16^*#	20.07±2.28^*#	8.88±0.38^*#
H-GLA+ML385	24.13±1.91^*#&	37.69±3.57^*#&	21.30±1.80^*#&
F值	326.027	450.754	319.287
P值	<0.001	<0.001	<0.001

Whitening effect of glabridin and its mechanism

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组别	HO-1 mRNA相对表达量	NQO-1 mRNA相对表达量
C	1.00±0.05	1.00±0.05
UVB	0.26±0.02^*	0.29±0.01^*
L-GLA	0.40±0.02^*#	0.47±0.03^*#
M-GLA	0.63±0.04^*#	0.63±0.05^*#
H-GLA	0.86±0.08^*#	0.91±0.08^*#
H-GLA+ML385	0.39±0.02^*#&	0.42±0.04^*#&
F值	539.106	373.148
P值	<0.001	<0.001

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