神经酰胺ⅢB磷脂复合物的制备及其评价

doi:10.3969/j.issn.1001-1803.2023.02.004

Abstract

Abstract:

Using ceramide ⅢB （Cer ⅢB） and lecithin as raw materials and anhydrous ethanol as reaction solvent, ceramide ⅢB phospholipid complex （CerPC） was prepared by evaporative solvent method. By orthogonal experiment, CerPC was prepared and confirmed according to the following method. Cer ⅢB （20.0 mg/mL） and phospholipids was added in anhydrous ethanol in a molar ratio of 1∶6 and stirred for 1.0 h at 60 ℃. The formation of CerPC was confirmed by using Fourier transform infrared （FT IR） spectroscopy, differential scanning calorimetry （DSC）, and scanning electron microscopy （SEM）. Permeability and retention were analyzed by an in vitro diffusion test in a Franz diffusion cell with a Strat-M^® membrane. Safety and irritation were analyzed by a chick embryo chorioallantois membrane （CAM） assay. The inhibition rate of CerPC on inflammatory factor COX-2 was determined by using a COX-2 inhibitor screening kit. The results show that the preparation method of the phospholipid complex designed in this study is safe and effective, and thus can be used as a drug delivery system for Cer ⅢB to improve its bioavailability and expand its application field.

Key words: ceramide ⅢB, phospholipid, structure, solubility, safety, permeability

CLC Number:

TQ658

Song Guanjie,Wu Wenhai,Zhang Huirong,Wu Yuehang,Yi Fan,Li Li. Preparation and evaluation of ceramide ⅢB phospholipid complex[J].China Surfactant Detergent & Cosmetics, 2023, 53(2): 149-156.

Figures/Tables 12

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References 23

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项目	复合时间/h
项目	0.5	1	2	3
复合率/%	86.10±0.44	88.24±0.89	90.34±0.80	90.06±2.38

项目	投料比（n（Cer ⅢB）∶n（PC））
项目	1∶1	1∶2	1∶3	1∶6	1∶8
复合率/%	41.00±4.03	64.20±0.89	88.60±0.89	92.80±1.05	92.60±2.06

项目	质量浓度/（mg/mL）
项目	10	20	25	30	40
复合率/%	81.80±2.95	84.40±1.11	88.60±0.89	64.40±2.59	73.80±3.36

项目	复合温度/℃
项目	30	40	50	60
复合率/%	82.80±2.36	88.60±0.89	81.40±2.08	89.20±1.09

编号	A/h	B/ （mg/mL）	C	D/℃	复合率/%
1	1	10	1∶3	30	65.4%
2	1	20	1∶6	40	89.6%
3	1	25	1∶8	60	93.2%
4	2	10	1∶6	60	85.2%
5	2	20	1∶8	30	92.6%
6	2	25	1∶3	40	88.4%
7	3	10	1∶8	40	76.8%
8	3	20	1∶3	60	93.0%
9	3	25	1∶6	30	90.9%
K₁	2.482 0	2.273 6	2.468 0	2.508 6
K₂	2.682 0	2.772 0	2.656 6	2.547 6
K₃	2.606 2	2.724 6	2.645 6	2.714 0
R	0.200 0	0.498 4	0.188 6	0.205 4

Preparation and evaluation of ceramide ⅢB phospholipid complex

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样品名称	样品中Cer ⅢB含量为0.25%时样品的 COX-2抑制率/%
Cer ⅢB	51.95±0.76
PM	93.32±0.53
CerPC	98.74±0.68