Abstract:

A skin sensitizer refers to a substance that will lead to an allergic response following repeated skin contact as defined by the United Nations Globally Harmonized System of Classification and Labelling of Chemicals. The current knowledge of the chemical and biological mechanisms associated with skin sensitisation has been summarised as an Adverse Outcome Pathway （AOP） starting with a molecular initiating event through intermediate events to the adverse effect, namely allergic contact dermatitis. Using cysteine and lysine derivatives instead of skin tissue, simulating the reaction of skin proteins with compounds, classifying the substances by calculating the percentage of depletion value after the reaction of cysteine and lysine derivatives to distinguish skin sensitizers and desensitizers, this method is called Amino acid Derivative Reactivity Assay （ADRA）. In this study, ten known allergens were selected to explore the accuracy of the sensitivity evaluation of ADRA method, and verified accuracy by DPRA that several substances were randomly selected among the ten substances. The results show that the sensitivity determination results of 10 substances are consistent with the sensitivity of samples, and the accuracy of determination of ADRA sensitivity is good.

Key words: skin sensitisation, alternatives experiment, ADRA