复合活性物促进毛发生长作用的研究

doi:10.3969/j.issn.1001-1803.2022.01.008

Abstract

Abstract:

Astragalus polysaccharide （APS） was used as the main raw material to prepare the Complex active substance （CAS）. The anti-inflammatory and hair-growth promoting activities of CAS were determined by in vitro cell and animal experiments, and the mechanism of action was preliminarily analyzed. The results showed that the content of APS prepared by this method was 86.21%. The results of animal experiment showed that the CAS significantly increased the number of hair follicles and the thickness of the dermis in the alopecia area of mice （p<0.05）, and prolonged the hair growth period. The mechanism might be that the expression of TGF-β1 and IL-1β could be down-regulated to delay hair follicles entering the resting period, and the expression of VEGF in skin tissue could be up-regulated to promote hair-follicle angiogenesis, improve nutrient absorption of hair follicles and thus play a role in promoting hair growth. In addition, the results of in vitro cell experiments showed that the CAS and APS had no cytotoxicity at the investigated concentration, and showed significant concentration-dependent inhibitory effects on LPS-induced NO and IL-1β, suggesting that CAS and APS had good anti-inflammatory activity. Compared with Astragalus polysaccharide, CAS could significantly shorten the time taking for hair growth on the hair-removed skin in mice, and could significantly increase the thickness of the dermis and the number of follicles. Furthermore, CAS could significantly inhibit TGF-β1 expression compared with APS. The animal experiments confirmed that the CAS could inhibit inflammation factor and promote hair growth, which was better than single Astragalus polysaccharides. Moreover, the components of the CAS might have synergistic effects. In conclusion, the CAS could effectively inhibit the inflammatory reaction and promote hair growth, which could be used as active ingredients in daily cosmetics with functions of relieving skin inflammation, preventing hair loss, hair conditioning and hair care.

Key words: Complex active substance, Astragalus polysaccharide, polypeptide, hair growth, anti-inflammatory

CLC Number:

TQ658

Li Honghai,Han Ping,Ma Shijing,Xiang Qin,Tang Zhongbiao,Du Zhiyun. Study on the hair-growth promotion effect of Complex active substance[J].China Surfactant Detergent & Cosmetics, 2022, 52(1): 50-55.

Figures/Tables 7

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References 22

[1]	Yuan Y M, Zeng Y S, Mao S Q. Application prospect of six common Chinese herbal medicines in anti-hair loss products[J]. China Surfactant Detergent & Cosmetics, 2019, 49(10) : 674-680.
[2]	Avram M R, Cole J P, Chase C, et al. The potential role of minoxidil in the hair transplantation setting[J]. Dermatologic Surgery, 2010, 28(10) : 894-900.
[3]	Wu S, Zhang H Z. Progress on the pathologic mechanism and potential therapy of androgenic alopecia[J]. Detergent & Cosmetics, 2012, 35(2) : 29-33.
[4]	Hou C, Miao Y, Wang J, et al. Collagenase Ⅳ plays an important role in regulating hair cycle by inducing VEGF, IGF-1, and TGF-β expression[J]. Drug Design Development and Therapy, 2015, 9:5373-5383.
[5]	Harmon C S, Nevins T D. IL-1 alpha inhibits human hair follicle growth and hair fiber production in whole-organ cultures[J]. Lymphokine and Cytokine Research, 1993, 12(4) : 197-203. pmid: 8218592
[6]	Boisvert W A, Yu M, Choi Y, et al. Hair growth-promoting effect of Geranium sibiricum extract in human dermal papilla cells and C57BL/6 mice[J]. Bmc Complementary and Alternative Medicine, 2017, 17(1) : 109. doi: 10.1186/s12906-017-1624-4 pmid: 28193226
[7]	Jiang C L, Tang C, Qiang Y, et al. Immunoregulation effect of Astragalus polysaccharides[J]. Food Science, 2013, 34(11) : 327-332.
[8]	Yue H Y, Qin Y L, Li P, et al. Comparison of the flavonoids contents and antioxidant activities in different parts of Astragali Radix[J]. Journal of Chinese Medicinal Materials, 2020 (12) : 2901-2904.
[9]	Duan H L, Cao R B, Zang K H, et al. Study on the regulatory mechanism of Astragalus membranaceus on tumor microenvironment of NSCLC Based on network pharmacology[J]. Pharmacology and Clinics of Chinese Materia Medica, 2020, 36(5) : 120-126.
[10]	Zhao Y L, Fan W X. Promoted murine hair growth by some Chinese herbs in vivo[J]. Journal of Clinical Dermatology, 2003 (6) : 321-322.
[11]	Jing L, Yu Z J, Hu T T, et al. Pharmacodynamics of Buqi Huoxue Shengfa tincture in treating model of sodium sulfide hair removal in mice[J]. Journal of Chengdu University of TCM, 2020, 43(2) : 31-36.
[12]	Curtis A, Calabro K, Galarneau J R, et al. Temporal variations of skin pigmentation in C57BL/6 mice affect optical bioluminescence quantitation[J]. Molecular Imaging and Biology, 2011, 13(16) : 1114-1123. doi: 10.1007/s11307-010-0440-8
[13]	Liu L, Chen Y Y, Zhang L, et al. Experimental study on the effects of the extract of Ginseng Radix et Rhizoma on hair growth of C57BL/6J mice[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2013, 19(7) : 250-254.
[14]	Liang Z J, Zhang J Y, Feng H P, et al. Optimization of extraction technology for polysaccharides of Astragalus with response surface methodology[J]. Food Research and Development, 2018, 39(21) : 72-76.
[15]	Wang Y Q, Meng X J, Li D N, et al. Study on removal of protein from polysaccharides of Schisandra chinensis Turcz.Bail.[J]. Science and Technology of Food Industry, 2011, 32(3) : 280-284.
[16]	Lu X, Min L, Wei J, et al. Heliangin inhibited lipopolysaccharide-induced inflammation through signaling NF-κB pathway on LPS-induced RAW264.7 cells[J]. Biomedicine & Pharmacotherapy, 2017, 88:102-108. doi: 10.1016/j.biopha.2017.01.041
[17]	Hooshmand S, Kumar A, Zhang J Y, et al. Evidence for anti-inflammatory and antioxidative properties of dried plum polyphenols in macrophage RAW264.7 cells[J]. Food & Function, 2015, 6(5) : 1719-1725.
[18]	Liu G R, Ma S J, Han P, et al. Optimization of extracting Tremella fuciform polysaccharide and its protection against SDS-induced damage of HaCaT cells[J]. Edible Fungi of China, 2021, 40(1) : 97-102.
[19]	Li R, Zhang N, Yao L, et al. Study on the hair growth promotion effect of lavender essential oil and Asian mint essential oil[J]. China Surfactant Detergent & Cosmetics, 2020, 50(8) : 536-541.
[20]	Kusumastuti S A, Nugrahaningsih D, Wahyuningsih M. Centella asiatica(L.) extract attenuates inflammation and improve insulin sensitivity in a coculture of lipopolysaccharide (LPS)-induced 3T3-L1 adipocytes and RAW264.7 macrophages[J]. Drug Discoveries & Therapeutics, 2019, 13(5) : 261-267.
[21]	Park A M, Khan S, Rawnsley J. Hair biology: growth and pigmentation[J]. Facial Plastic Surgery Clinics of North America, 2018, 26(4) : 415-424. doi: 10.1016/j.fsc.2018.06.003
[22]	Meephansan J, Thummakriengkai J, Ponnikorn S, et al. Efficacy of topical tofacitinib in promoting hair growth in non-scarring alopecia: possible mechanism via VEGF induction[J]. Archives of Dermatological Research, 2017, 309(9) : 729-738. doi: 10.1007/s00403-017-1777-5 pmid: 28884378

实验组	ρ/（μg/mL）	细胞活性/%	毒性分级
空白对照组	—	100.00±0.10	0
黄芪多糖	500	94.18±0.23	1
黄芪多糖	1 000	93.05±0.48	1
复合多肽（4 μg/mL^@）	500	98.86±0.16	1
复合多肽（4 μg/mL^@）	1 000	96.21±0.36	1
复合活性物	10	101.49±0.41	0
	50	98.85±1.23	1
	250	97.83±0.19	1
	500	99.22±0.23	1
	1 000	93.06±0.81	1

实验组	ρ/ （μg/mL）	NO含量/ （μmol/L）	IL-1β含量/ （pg/mg）
对照组	—	13.92±0.51	9.89±2.26
模型组（LPS）	1（LPS）	40.22±0.18^###	50.24±1.12
黄芪多糖	500	22.23±0.22^***	27.57±2.17^***
黄芪多糖	1 000	20.59±0.41^***	25.82±3.21^***
复合活性物	10	38.87±0.38	47.93±2.76
	50	35.96±0.11^*	45.19±3.82
	250	28.03±0.57^**	34.02±1.19^**
	500	22.87±0.33^***	26.38±1.28^***
	1 000	21.94±0.43^***	23.05±0.99^***

组别	T1皮肤变灰时间/d	T2皮肤长出毛发时间/d
空白对照组	11.4±1.35	15.2±1.91
阳性对照组	7.2±1.44^*	10.1±2.58^*
黄芪多糖组	8.6±1.16^*	12.5±1.29
复合活性物组	7.4±2.76^*	11.3±1.38^*

实验组	真皮厚度/μm	毛囊数量/个
空白对照组	375.5±26.5	57.5±13.2
阳性对照组	456.5±49.9^*	89.5±15.1^*
黄芪多糖组	421.7±21.0	87.0±16.2^*
复合活性物组	439.5±26.8^*	98.9±12.1^**

组别	TGF-β1含量/ （pg/mg）	IL-1β含量/ （pg/mg）	VEGF含量/ （pg/mg）
空白对照组	112.08±2.11	39.65±1.73	45.94±0.04
阳性组	105.77±2.72	32.14±2.93	67.61±2.34^**
黄芪多糖组	95.97±1.86	30.27±1.02^*	50.86±0.98^*
复合活性物组	49.58±1.73^***	23.27±3.69^**	61.33±1.74^**

Study on the hair-growth promotion effect of Complex active substance

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