响应面法优化水解制备柞蚕丝素肽及其性能

doi:10.3969/j.issn.1001-1803.2021.07.005

Abstract

Abstract:

Tussah fibroin peptides were prepared from tussah silk fibroin by hydrolysis method with phosphoric acid. On the basis of single-factor test, the preparation process was optimized using the response surface methodology. The effects of various factors on the recovery of tussah fibroin peptides were investigated, and the performance of samples obtained under the optimized conditions was analyzed. The results show that the optimal hydrolysis conditions are as follows: the mass fraction of liquid material is 9.93%; the hydrolysis temperature is 91.11 ℃; the hydrolysis time is 5.44 h; the theoretical recovery rate is 70.34%. The actual recovery rate can reach 69% which is consistent with the theoretical level. The optimized hydrolysis technology for preparation of tussah fibroin peptides was established using response surface methodology. The performance experiments of tussah fibroin peptides show that the sample has good hygroscopic and moisturizing efficacy, and functions of sunscreen and bacteriostasis. Tussah silk peptides have high application value in the development of natural skin care products.

Key words: tussah, fibroin peptide, hydrolysis process, response surface method

CLC Number:

TQ658

Mi Rui,Li Xuejun,Ma Shuhui,Meng Nan,Li Shuying,Du Xingfan. Optimization of hydrolysis preparation of tussah fibroin peptides by response surface methodology and the performance thereof[J].China Surfactant Detergent & Cosmetics, 2021, 51(7): 612-619.

Figures/Tables 12

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References 20

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水平	因素
水平	水解温度/℃ （A）	柞蚕丝素质量分数/% （B）	水解时间/h （C）
-1	85	9.5	3
0	90	10	5
1	95	10.5	7

方差来源	平方和	自由度	均方	F值	P值
模型	2 190.25	9	243.36	53.54	<0.000 1
A	786.06	1	786.06	172.95	<0.000 1
B	454.51	1	454.51	100.00	<0.000 1
C	137.78	1	137.78	30.31	0.000 9
AB	32.49	1	32.49	7.15	0.031 8
AC	16.40	1	16.40	3.61	0.099 3
BC	8.12	1	8.12	1.79	0.223 1
A²	321.26	1	321.26	70.68	<0.000 1
B²	229.63	1	229.63	50.52	0.000 2
C²	127.83	1	127.83	28.13	0.001 1
残差	31.82	7	4.55
失拟	25.13	3	8.38	5.01	0.076 8
纯误差	6.69	4	1.67
合计	2 222.06	16
相关系数r²	0.985 7
调整确定系数r²_adj	0.967 3
变异系数	2.13%

Optimization of hydrolysis preparation of tussah fibroin peptides by response surface methodology and the performance thereof

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试验编号	水解温度/℃ （A）	柞蚕丝素质量分数/% （B）	水解时间/h （C）	回收率/% （Y）
1	0	0	0	67.5
2	0	1	1	47.8
3	0	1	-1	42.3
4	0	-1	1	68.4
5	-1	0	-1	39.4
6	-1	1	0	38.6
7	-1	-1	0	45.3
8	0	0	0	65
9	0	0	0	67.4
10	0	0	0	66
11	0	0	0	68.2
12	1	-1	0	68.5
13	1	0	1	69.8
14	-1	0	1	43.6
15	1	0	-1	57.5
16	1	1	0	50.4
17	0	-1	-1	57.2

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