高效液相色谱法同时测定化妆品原料甘草提取液中甘草苷和甘草酸

doi:10.3969/j.issn.1001-1803.2021.03.013

Abstract

Abstract:

A comprehensive analytical method based on high performance liquid chromatography （HPLC） was developed for the determination of liquiritin and glycyrrhizic acid in licorice extract in cosmetics. The target compounds were extracted with 50% ethanol, separated on a Agilent ZORBAX SB-C₁₈ column （4.6 mm×250 mm×5 μm） in gradient elution program using acetonitrile and 0.05% phosphoric acid as mobile phases, at flow rate of 1 mL/min, and detection wavelength of 237 nm. The method had a short test time and accurate test results, it showed good linear relationship between 0.5-100 μg/mL with correlation coefficients larger than 0.999 5. The spiked recoveries in the blank sample were in the range of 91.47%-114.57% with the relative standard deviations of 0.87%-3.32%. The results indicate that the developed method is rapid, accurate and reliable, which is suitable for the determination of liquiritin and glycyrrhizic acid in licorice extract in cosmetics.

Key words: cosmetics raw materials, liquiritin, glycyrrhizic acid, high performance liquid chromatography （HPLC）

CLC Number:

TQ658

YANG Hong-mei,XU Jia-qi,GONG Sheng-zhao,YU Juan. Simultaneous determination of liquiritin and glycyrrhizic acid by high performance liquid chromatography in licorice extract in raw materials of cosmetics[J].China Surfactant Detergent & Cosmetics, 2021, 51(3): 250-255.

Figures/Tables 7

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Tab. 2

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References 13

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组分	线性范围/（μg·mL^-1）	线性回归方程	相关系数（r²）
甘草苷	0.5~100	y=14.759x+3.810	0. 999 9
甘草酸	0.5~100	y=5.583x+1.168	0. 999 8

	加入量/（μg·mL^-1）	实测值/（μg·mL^-1）	加标回收率/%	平均回收率/%	RSD/%
甘草苷	1	1.15	115	111.50	3.25
		1.16	116
		1.11	111
		1.08	108
		1.07	107	111.50	3.25
		1.12	112	111.50	3.25
甘草苷	10	11.55	115.5	114.57	1.54
		11.45	114.5
		11.63	116.3
		11.60	116.0
		11.17	111.7
		11.34	113.4
	20	22.21	111.05	112.38	0.87
		22.48	112.40
		22.58	112.90
		22.63	113.15
		22.27	111.35
		22.68	113.40
甘草酸	1	0.95	95	92.17	3.32
		0.97	97
		0.90	90
		0.91	91
		0.90	90
		0.90	90
	10	9.20	92.0	91.47	1.43
		9.0	90.0
		9.0	90.0
		9.34	93.4
		9.19	91.9
		9.15	91.5
	20	19.34	96.70	97.66	1.96
		19.63	98.15
		19.61	98.05
		19.79	98.95
		19.95	99.75
		18.87	94.35

Simultaneous determination of liquiritin and glycyrrhizic acid by high performance liquid chromatography in licorice extract in raw materials of cosmetics

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t/min	V（乙腈）/%	V（0.05%磷酸）/%
0	25	75
1	25	75
12	60	40
13	25	75
16	25	75