含吡啶杂环氯化铵阳离子表面活性剂的杀菌活性

doi:10.3969/j.issn.1001-1803.2020.05.006

Abstract

Abstract:

The purpose is to screen fungicides with good water solubility, environmental protection and strong bactericidal effect on plant pathogens. The bactericidal activity of pyridine-containing heterocyclic ammonium chloride cationic surfactants with different carbon chain lengths （C8, C10, C12, C14, C16） against four kinds of plant pathogens was tested by agar plate dilution method. The toxicity regression equation was obtained, and the effective antibacterial concentration （EC₅₀） was calculated. The results show that the pyridine-containing heterocyclic ammonium chloride cationic surfactants with a carbon chain length of 12 （C12） has a significant antibacterial activity for Rhizoctonia solani Kühn, watermelon Fusarium wilt, apple valsa canker and Botrytis cinerea Pers. with the EC₅₀ of 9.69×10 ^-3, 2.93×10 ^-2, 1.57×10 ^-5 and 1.85×10 ^-4g/L, respectively. While the EC₅₀of the control drugs containing jinggangmycin, methyl butazine, difenoconazole and mycoazole for the above plant pathogens individually are 7.02×10 ^-2, 0.506, 3.50×10 ^-4 and 2.04×10 ^-3 g/L, respectively. The EC₅₀ value of C12 for four pathogens are significantly lower than the control drugs, which has good bactericidal activity and further research value. The minimal inhibitory concentration （MIC） of C12 against the four plant pathogens are 0.094, 0.091, 0.083 and 0.087 g/L, respectively. It provides the basis for the research on the prevention and treatment of plant pathogens by pyridine-containing heterocyclic ammonium chloride cationic surfactants.

Key words: pyridine-containing heterocyclic ammonium chloride cationic surfactants, plant pathogens, in vitro, minimum inhibitory concentration, fungicidal activity

CLC Number:

TQ423.12

LI Yun-qing,WANG Li-yan,TANG Li,LIU Jia-qing,DU Yu-ying,WU Ding-cheng. Bactericidal activity of pyridine-containing heterocyclic ammonium chloride cationic surfactants[J].China Surfactant Detergent & Cosmetics, 2020, 50(5): 314-318.

Figures/Tables 5

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References 25

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表面活性剂	菌丝生长抑制率/%
表面活性剂	0.005 g/L	0.020 g/L	0.080 g/L
C8	31.14	35.28	51.81
C10	36.51	46.83	81.03
C12	46.33	58.05	92.37
C14	36.30	47.54	87.30
C16	34.94	40.18	77.03

化合物	毒力回归方程	EC₅₀/（g·L^-1）
C8	y=2.756 1 x +0.297 7	7.34×10^-2
C10	y=6.876 5 x +0.330 8	2.46×10^-2
C12	y=7.814 9 x +0.424 2	9.69×10^-3
C14	y=7.478 1 x +0.325 9	2.25×10^-2
C16	y=3.493 3 x +0.331 9	4.81×10^-2
井冈霉素	—	7.02×10^-2

表面活性剂	毒力回归方程
表面活性剂	西瓜枯萎病原菌	苹果腐烂病原菌	番茄灰霉病原菌
C8	y = 1.815 0 x +0.100 9	y =4.305 6 x +0.274 4	y =5.468 0 x +0.287 6
C10	y = 6.615 7 x +0.056 42	y =6.132 0 x +0.462 5	y =4.289 7 x +0.435 6
C12	y =10.450 5 x +0.193 8	y =6.336 4 x +0.499 9	y =6.500 4 x +0.498 8
C14	y = 4.108 4 x +0.085 77	y =4.888 3 x +0.465 1	y =4.798 2 x +0.437 9
C16	y = 3.311 2 x +0.115 9	y =4.146 7 x +0.341 2	y =4.922 0 x +0.397 8

化合物	EC₅₀/（g·L^-1）
化合物	西瓜枯萎病原菌	苹果腐烂病原菌	番茄灰霉病原菌
C8	0.219	5.24×10^-2	3.88×10^-2
C10	6.70×10^-2	6.11×10^-3	1.50×10^-2
C12	2.93×10^-2	1.57×10^-5	1.85×10^-4
C14	8.57×10^-2	7.13×10^-3	1.29×10^-2
C16	0.116	3.83×10^-2	2.08×10^-2
甲基托布津	0.506	—	—
苯醚甲环唑	—	3.50×10^-4	—
菌恶唑	—	—	2.04×10^-3

Bactericidal activity of pyridine-containing heterocyclic ammonium chloride cationic surfactants

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