氨基酸型复合纳米乳抑菌性能的研究

doi:10.3969/j.issn.1001-1803.2020.05.005

Abstract

Abstract:

The buckwheat flavone nanoemulsion system was constructed with amino acid surfactant sodium lauroyl sarcosinate and PEG-40 hydrogenated castor oil as the composit emulsifiers, 1,2-propylene glycol as co-emulsifier and castor oil as oil phase, and its bacteriostatic activity was evaluated. The results showed that the nanoemulsion was transparent and clear in appearance, with uniform particle size distribution. The PDI, surface tension and pH value all showed that the nanoemulsion system was good. In vitro release performance showed that the cumulative release of nanoemulsion was significantly better than that of control suspension within 24 h. The minimum inhibitory concentration （MIC） and the minimum bactericidal concentration （MBC） of nanoemulsion and suspension to Staphylococcus aureus and Escherichia coli showed that the bacteriostatic activity of nanoemulsion was significantly higher than that of suspension. The bacteriostatic rates of nanoemulsion against Staphylococcus aureus and Escherichia coli were 92.1%±1.93% and 93.2%±1.26%, respectively, and those of the suspension of the same concentration against Staphylococcus aureus and Escherichia coli were 42.0%±1.58% and 45.6%±2.33%, respectively.

Key words: amino acid surfactant, nanoemulsion, in vitro release, bacteriostatic properties

CLC Number:

TQ423

ZHAO Zhi-juan,GONG Pei-long,CUI Xiao-dong,WANG Zhuan-hua. Bacteriostatic properties of amino acid composite nanoemulsion[J].China Surfactant Detergent & Cosmetics, 2020, 50(5): 309-313.

Figures/Tables 6

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Tab. 1

Fig. 2

Tab. 2

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Tab. 4

References 15

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分组	pH	表面张力/（mN·m^-1）
1	6.89±0.01	33.15±0.42
2	6.75±0.01	34.17±0.63
3	6.91±0.01	33.57±0.66

ρ/（mg·L^-1）	空白纳米乳		混悬液		纳米乳
ρ/（mg·L^-1）	大肠杆菌	金黄色葡萄球菌	大肠杆菌	金黄色葡萄球菌	大肠杆菌	金黄色葡萄球菌
0.39	+	+	+	+	+	+
0.78	+	+	+	+	+	+
1.56	+	+	+	+	+	+
3.12	+	+	+	+	+	+
6.25	+	+	+	+	-	+
12.50	+	+	-	+	-	-
25.00	+	+	-	-	-	-
50.00	+	+	-	-	-	-
100.00	+	+	-	-	-	-
阴性对照	-	-	-	-	-	-
阳性对照	+	+	+	+	+	+

菌种	ρ（纳米乳）/（mg·L^-1）		ρ（混悬液）/（mg·L^-1）
菌种	MIC	MBC	MIC	MBC
大肠杆菌	6.25	12.50	12.50	50.00
金黄色葡萄球菌	12.50	50.00	25.00	100.00

菌种	纳米乳抑菌率/%			混悬液抑菌率/%
菌种	第一次	第二次	第三次	第一次	第二次	第三次
大肠杆菌	91.5	94.5	93.6	42.6	48.3	45.8
金黄色葡萄球菌	89.6	94.3	92.4	39.8	43.5	42.6

Bacteriostatic properties of amino acid composite nanoemulsion

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