Abstract:

The fatty acid composition of tea seed oil and Camellia emollient oil, its antioxidant capacity in vitro and its effect on the skin of aging mice were determined and compared. The fatty acid composition of tea seed oil and Camellia emollient oil was analyzed and compared by gas chromatography and mass spectrometry. Antioxidant activities of tea seed oil and Camellia emollient oil in vitro were determined by DPPH and ABTS scavenging methods. A mouse aging model was established by subcutaneous injection of 1 g/（kg·d）D-galactose solution in the back. The skin was applied with tea seed oil and Camellia emollient oil to observe superoxide dismutase（SOD）, content of malondialdehyde（MDA）, hydrogen peroxidase（CAT）, content of glutathione peroxidase（GSH-Px）, content of hydroxyproline（Hyp）and morphological changes in it. From the results, both unsaturated fatty acids are rich in oleic acid and linoleic acid. Both of them have antioxidant capacity in vitro and these effects are does-dependent. In vivo experiment, compared with the normal group, the SOD, CAT, GSH-Px, Hyp contents of the skin of the model group decrease, the MDA content increases, the thickness of the epidermis and dermis decrease, and the collagen fiber content decreases. After in vitro intervention, the SOD, CAT, GSH-Px, and Hyp contents of the skin of the mice increase, the MDA content decreases, the thickness of the epidermis and dermis increase, and the collagen fibers are abundant. The anti-oxidation experiment on the combination of in vitro and in vivo of tea seed oil and Camellia emollient oil show that they both have certain effect on delaying skin aging.

Key words: tea seed oil, Camellia emollient oil, antioxidant in vitro, free radical, scavenging effect, antioxidant in vivo