HPLC-MS/MS测定化妆品中5种禁用胆碱类化合物

doi:10.13218/j.cnki.csdc.2018.04.011

Abstract

Abstract: A method was developed for the determination of five kinds of choline compounds prohibited in cosmetics by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).The samples were oscillated and extracted with methanol-water solution (V(CH₃OH)∶V(H₂O)=3∶1) assisted by vortex and ultrasonic.Then,after centrifugation,the five target compounds were separated by Welch Ultimate XB-C₁₈ column (4.6 mm×150 mm,5 μm).The analytes were ionized by electrospray ionization in positive mode (ESI⁺) and detected under MRM mode.Under the optimal conditions,the five choline compounds prohibited are all well separated with good symmetry of peaks.The choline salicylate shows good linear relationship in the concentration range of 10.0-80.0 μg/L with correlation coefficient of 0.999 1.Other choline compounds show good linear relationship in the concentration range of 1.0-40.0 μg/L with the correlation coefficients larger than 0.995 0.The limits of detection and quantification (LOD and LOQ) of the method are in the range of 2.0-30.0 μg/kg and 6.0-90.0 μg/kg,respectively.The recovery experiments of spiked samples show that,the recoveries of the five choline compounds are in the range of 85.4%-103.9% with RSDs ranging from 1.9% to 6.9% (n=6).

Key words: cosmetics, choline compound, HPLC-MS/MS

CLC Number:

TQ658

ZHANG Rui-rui, DU Wei-feng, CEN Jian-bin, OU Shuo-jun, XUN Zhi-qing, HUANG Jin-feng. Determination of five kinds of choline compounds prohibited in cosmetics by HPLC-MS/MS[J].China Surfactant Detergent & Cosmetics, 2018, 48(4): 237-242.

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Determination of five kinds of choline compounds prohibited in cosmetics by HPLC-MS/MS

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