诺丽精油微乳体系的构建及其稳定性和抑菌活性研究

doi:10.3969/j.issn.2097-2806.2025.09.005

Abstract

Abstract:

In order to obtain stable oil-in-water （O/W） microemulsion of Noni essential oil （NEO）, the water titration method and the pseudo ternary phase diagram method were used to investigate the effects of polyoxyethylene nonionic surfactants, co-surfactants, Km value （the mass ratio of surfactant to co-surfactant）, components, and proportions on the microemulsion formation. Meanwhile, the particle size, morphology, electrical conductivity, water solubility, stability, and antifungal activity of the NEO microemulsion were measured to determine their characteristics and physicochemical properties. The results show that castor oil polyethylene glycol 40 as the surfactant, propylene glycol as the co-surfactant, and Km with 3 when the mass ratio of NEO to the mixed surfactant is 7∶3, the water content of the O/W type aqueous phase is 70%, and the NEO microemulsion can be formed. The microemulsion is stable, transparent, and clear with a blue tint. The average particle size is （25.88±4.82） nm with the PDI of 0.177±0.030, and the microemulsion particles are more uniformly dispersed, spherical, and nearly spherical. At the same time, NEO microemulsion has good storage stability, centrifugal stability, and ion concentration stability, and is suitable for use at temperatures below 40 ℃ and in slightly acidic environments. The antifungal effect of NEO microemulsion on Candida tropicalis is better than that of NEO. This study shows that the microemulsion can achieve nanoparticle encapsulation of NEO, providing a good foundation for the widespread application of NEO.

Key words: Noni essential oil, microemulsion, pseudo ternary phase diagram, stability, Candida tropicalis, antifungal activity

CLC Number:

TQ658

Xuefeng Wang, Qian Jiang, Zuowang Fan, Xiaoli Xie, Fulai Yu, Dan Wang. Study on the construction, stability and antifungal activity of microemulsion system of Noni essential oil[J].China Surfactant Detergent & Cosmetics, 2025, 55(9): 1120-1128.

Figures/Tables 17

Tab. 1

Fig. 1

Tab. 2

Fig. 2

Tab. 3

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Tab. 4

Tab. 5

Tab. 6

Tab. 7

Tab. 8

Tab. 9

Fig. 8

References 23

[1]	张敏, 陈灿言, 方雅静. 两种不同产地诺丽果的主要营养成分与抗氧化活性评价[J]. 食品安全质量检测学报, 2022, 13 (18) : 5902-5909.
[2]	Jahurul M, Patricia M, Shihabul A, et al. A review on functional and nutritional properties of noni fruit seed (Morinda citrifolia L.) and its oil[J]. Food Biosci, 2021, 41: 101000.
[3]	晏永球, 童应鹏, 陆雨, 等. 诺丽的化学成分及药理活性研究进展[J]. 中草药, 2017, 48 (9) : 1888-1905.
[4]	徐雪峰, 谢普军, 闫浩. 诺丽果资源开发与利用研究进展[J]. 南方农业, 2023, 17 (17) : 191-194.
[5]	Mathivanan N, Surendira G, Srinivasan K. Review on the current scenario of Noni research: Taxonomy, distribution, chemistry, medicinal and therapeutic values of Morinda citrifolia L.[J]. Int. J. Noni. Res., 2005, 1: 1-16.
[6]	王丹, 胡璇, 于福来, 等. 一种诺丽精油在抑制奇异变形杆菌中的应用: CN115746957A[P]. 2023-03-07.
[7]	王丹, 江芊, 胡璇, 等. 基于HS-SPME-GC/MS的海南万宁产诺丽鲜果、干果和发酵液挥发性成分分析[J]. 中国调味品, 2022, 47 (12) : 162-167, 185.
[8]	张运起, 刘一婷, 李立新. 热带假丝酵母菌的生化特性与耐药性分析[J]. 临床合理用药杂志, 2016, 9 (16) : 132-133.
[9]	孙艳, 黄俊霞, 高燕, 等. 成人急性白血病并热带假丝酵母菌性败血症临床特点[J]. 青岛大学学报(医学版), 2020, 56 (4) : 464-468.
[10]	谢小丽, 胡璇, 黄英, 等. 益智精油O/W型微乳液的制备及其性能研究[J]. 日用化学工业(中英文), 2024, 54 (10) : 1201-1210.
[11]	石长波, 徐朔, 赵钜阳, 等. 食品中自微乳体系的组成、制备及促进生物活性物质生物利用的研究进展[J]. 食品工业科技, 2024, 45 (17) : 426-435.
[12]	墙梦捷, 鲁晓翔. 艾叶精油微乳的表征及稳定性研究[J]. 食品与发酵工业, 2020, 46 (23) : 86-91.
[13]	张佩华, 梅子, 傅玉颖. 微乳的稳定性研究[J]. 食品工业科技, 2014, 35 (7) : 73-79.
[14]	王军. 微乳液的制备及其应用[M]. 北京: 中国纺织出版社: 2011: 12.
[15]	Nirmal N P, Chunhavacharatorn P, Khanashyam A C, et al. Cinnamon bark oil in water nanoemulsion formulation, characterization, and antimicrobial activities[J]. LWT, 2023, 179: 114671.
[16]	褚祚晨. 草豆蔻精油微乳液的制备及对香蕉保鲜的影响[D]. 武汉: 华中农业大学, 2022.
[17]	程晨, 司红燕, 王宗德, 等. O/W型山苍子精油微乳的制备及其性能研究[J]. 林产化学与工业, 2021, 41 (1) : 67-76.
[18]	石俊杰. 百里香精油微乳的制备及结合1-MCP在枇杷保鲜中应用[D]. 天津: 天津商业大学, 2022.
[19]	Dan Y, Liyan S, Ying S, et al. Formulation and evaluation of a α-linolenic acid and vitamin E succinate microemulsion with low surfactant content and free of co-surfactant for use as a nutritional supplement[J]. Food Chemistry, 2021, 364: 130433.
[20]	周雪, 姜丰, 严俊丽, 等. 薄荷油微乳凝胶的制备研究[J]. 时珍国医国药, 2018, 29 (3) : 587-591.
[21]	于泽权. 槐糖脂-依托泊苷亚微乳的制备及其体内外评价[D]. 合肥: 合肥工业大学, 2019.
[22]	韩冰, 郑野, 徐嘉, 等. 微乳体系的制备及其稳定性研究进展[J]. 食品与发酵工业, 2020, 46 (24) : 284-291.
[23]	彭群, 段翰英, 叶之壮, 等. 重组乳液粒径对甜橙油纳米微胶囊稳定性的影响[J]. 食品与发酵工业, 2012, 46 (16) : 56-61.

表面活性剂	SMC和NEO质量比				微乳区面积
表面活性剂	9∶1	8∶2	7∶3	6∶4	微乳区面积
蓖麻油聚氧乙烯醚40	澄清透明	澄清透明	澄清透明，呈现蓝光	浑浊	0.863
脂肪醇聚氧乙烯醚羧酸	澄清透明	澄清透明，略有蓝光	浑浊	浑浊	0.817
脂肪醇聚氧乙烯醚9	澄清透明	较澄清	浑浊	浑浊	0.830
腰果酚聚氧乙烯醚10	澄清透明	浑浊	浑浊	浑浊	0.860

助表面活性剂	SMC和NEO质量比			微乳区面积
助表面活性剂	9∶1	8∶2	7∶3	微乳区面积
丙三醇	澄清透明	澄清透明	澄清透明，呈现蓝光	0.863
1,4-丁二醇	澄清透明	澄清透明	浑浊	0.718
聚乙二醇	澄清透明	浑浊	浑浊	0.722
异戊醇	澄清透明	澄清透明	浑浊	0.655

Km	SMC和NEO质量比			微乳区面积
Km	9∶1	8∶2	7∶3	微乳区面积
1	澄清透明	澄清透明，蓝光	浑浊	0.755
2	澄清透明，略微呈现蓝光	浑浊	浑浊	0.791
3	澄清透明	澄清透明	澄清透明，呈现蓝光	0.863
4	澄清透明	浑浊	浑浊	0.856

温度/℃	透光率/%
温度/℃	70%含水量微乳	90%含水量微乳
30	90.68±0.43 ^A	102.57±6.24 ^a
40	77.18±0.71 ^B	80.62±2.16 ^b
50	44.46±1.06 ^C	78.86±1.61 ^b
60	29.93±0.22 ^D	77.83±0.57 ^b
70	10.17±0.5 ^E	68.39±4.24 ^c
80	4.98±0.07 ^F	59.88±0.11 ^d
90	2.99±0.07 ^G	55.92±1.88 ^d
100	3.42±0.06 ^G	45.51±0.89 ^e

离心速度/（r/min）	透光率/%
离心速度/（r/min）	70%含水量微乳	90%含水量微乳
2 000	100.51±1.01^A	108.20±0.00 ^a
4 000	92.75±0.91 ^C	95.67±1.39 ^bc
6 000	95.17±0.45 ^BC	87.36±2.07 ^c
8 000	96.08±0.46 ^B	89.87±5.34 ^c
10 000	101.30±2.81 ^A	100.62±6.05 ^b

Study on the construction, stability and antifungal activity of microemulsion system of Noni essential oil

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 17

References 23

Related Articles 15

Metrics

Comments

Recommended 10

pH值	透光率/%
pH值	稀盐酸和氢氧化钠缓冲液	柠檬酸和磷酸二氢钠缓冲液
2	92.54±0.69 ^C	95.39±3.74 ^a
3	94.99±1.39 ^B	132.15±1.83 ^a
4	91.92±2.24 ^C	69.93±4.80 ^c
5	36.73±0.43 ^D	36.69±0.70 ^e
6	108.27±0.76 ^A	42.82±2.10 ^d
7	32.49±0.46 ^E	34.02±0.81 ^e
8	33.69±0.43 ^E	35.51±0.88 ^e
9	36.03±1.41 ^D	33.21±0.87 ^e

c（NaCl）/（mol/L）	平均粒径/nm	PDI
0	28.35±5.23 ^B	0.17±0.02 ^c
0.2	35.77±6.99 ^AB	0.32±0.05 ^a
0.4	36.34±13.98 ^AB	0.23±0.04 ^b
0.6	37.92±3.74 ^AB	0.33±0.03 ^a
0.8	28.24±1.39 ^B	0.22±0.03 ^bc
1	47.44±13.64 ^A	0.28±0.05 ^a

时间/d	透光率/%
时间/d	70%含水量微乳	90%含水量微乳
15	202.89±1.26 ^A	191.73±4.36 ^a
30	176.60±1.57 ^B	154.55±7.92 ^b
45	103.70±0.64 ^C	118.54±4.47 ^c
60	98.90±4.44 ^D	100.78±7.06 ^d

抑菌圈平均直径/mm				微乳溶剂对照组	溶剂对照组	阴性对照组
微乳A组	微乳B组	诺丽精油组	阳性对照组	微乳溶剂对照组	溶剂对照组	阴性对照组
22.25±1.33	17.73±1.21	15.37±1.28	20.71±0.70	—	—	—

[1]	Yibo Li, Min Yang, Qinghuan Deng, Shuoshi Wang. Study on the mechanism of in-situ viscosity reduction and wettability control in oil displacement by diluted-microemulsion [J]. China Surfactant Detergent & Cosmetics, 2025, 55(9): 1093-1099.
[2]	Yaokun Xiong, Si Yang, Shixi Hong, Haolang Zhang, Liangji Li, Yuhuan Chen. Research on the preparation process and pharmacodynamic effect of compound essential oil-lactic acid anti-hair loss microemulsion [J]. China Surfactant Detergent & Cosmetics, 2025, 55(7): 844-852.
[3]	Xiaoqian Jiang, Liya Zhang, Aixin Song, Xiuping Sun. Preparation and properties of the nanoemulsions and nanoemulsion gels stabilized with rhamnolipid [J]. China Surfactant Detergent & Cosmetics, 2025, 55(6): 677-686.
[4]	Yueling Xu, Xinfeng Dong, Xianyan Zhu, Ge Yang, Ruishuang Xu, Wei Tang, Li Yu. Preparation and properties of O₁/W/O₂ double emulsion loaded with menthol [J]. China Surfactant Detergent & Cosmetics, 2025, 55(6): 747-755.
[5]	Jun Li, Han Wu. Study on the preparation and properties of graphene-polyvinyl chloride composites [J]. China Surfactant Detergent & Cosmetics, 2025, 55(5): 611-615.
[6]	Pengpeng Li, Wuwei Li. Preparation and properties of biodegradable polyester materials for packaging [J]. China Surfactant Detergent & Cosmetics, 2025, 55(5): 625-632.
[7]	Fei Yan, Cheng Ma, Zhicheng Xu, Qingtao Gong, Lei Zhang, Lu Zhang. Study on the foam properties of extended anionic surfactants [J]. China Surfactant Detergent & Cosmetics, 2025, 55(4): 415-421.
[8]	Kaiyuan Miao, Zeyao Ouyang, Xinying Zhao, Xiaojing Yang, Xinyan Chen, Zhaohua Shi. Preparation, characterization, and whitening activity research of oxyresveratrol-loaded self-microemulsion [J]. China Surfactant Detergent & Cosmetics, 2025, 55(4): 453-463.
[9]	Li He, Huabin Li, Gang He, Lei Luo. Study on the influencing factors for the droplet size and stability of crude oil emulsions [J]. China Surfactant Detergent & Cosmetics, 2025, 55(3): 341-348.
[10]	Keke Zhang, Huanjin Zou, Ye Fan, Yongmin Zhang, Xuefeng Liu, Yinjun Fang. Preparation of highly stable O/W Pickering emulsion based on amphiphilic Janus-SiO₂ particles [J]. China Surfactant Detergent & Cosmetics, 2025, 55(3): 271-278.
[11]	Haibing Cheng, Yong Zhang, Lei Sun, Weijia Li, Zhi Chen, Congming Tang, Li Chang. Synthesis and crosslinking properties of W-SN crosslinking agent [J]. China Surfactant Detergent & Cosmetics, 2025, 55(3): 279-285.
[12]	Xiaoyu Li,Jianping Jiang,Lifeng Xiao. Performance analysis of nanostructured lipid carriers for chemical sunscreen agents [J]. China Surfactant Detergent & Cosmetics, 2025, 55(2): 185-193.
[13]	Yanfu Zhou, Xuguang Wang, Feifei Wang. Study on the foam properties of common surfactants [J]. China Surfactant Detergent & Cosmetics, 2025, 55(1): 26-33.
[14]	Meiling Shi, Juanbo Chen, Hao Li, Yuanqin Huang, Qiqing Zhang, Sa Zeng, Tao Meng. Study on the silicon dioxide encapsulation for the enhancement of vitamin C stability [J]. China Surfactant Detergent & Cosmetics, 2024, 54(9): 1015-1022.
[15]	Jian Wang, Yuhui Fan, Danfeng Li, Ningwen Cheng, Ling Li, Yufeng Yu. Skin care efficacy study of recombinant humanized collagen based on in vitro level [J]. China Surfactant Detergent & Cosmetics, 2024, 54(9): 1030-1038.