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2026年, 第56卷, 第5期　刊出日期：2026-05-22 上一期    下一期

科技讲座

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黏弹性表面活性剂（V）——蠕虫状胶束与聚合物的相似点及差异性 Viscoelastic surfactants (V) Similarity and difference between wormlike micelles and polymers 冯玉军 2026 (5):  557-563.  doi: 10.3969/j.issn.2097-2806.2026.05.001 摘要 ( 107 )   HTML ( 15 )   PDF(2312KB) ( 89 )   蠕虫状胶束可通过自组装形成类聚合物长链缠结结构，宏观呈现优异黏弹性流变特征，被称为“活聚合物”，在流变改性领域与传统聚合物存在诸多表观共性。本文主要对比了C22-尾基EDAB两性表面活性剂蠕虫状胶束与超高分子量（12×106 g/mol）非离子聚丙烯酰胺（PAM）以及十六烷基氯化吡啶/水杨酸钠（CPyCl/NaSal）蠕虫状胶束与聚苯乙烯两组体系，从宏观流变、微观结构、动力学机制多维度系统对比二者的相似特征与本质差异。结果表明，二者在长链拓扑结构、缠结增稠机制、温变流变规律上高度重合，但组装方式、结构稳定性、弛豫机制存在根本性区别。蠕虫状胶束为非共价动态平衡体系，具备双重弛豫机制与灵敏环境响应性，增稠效率显著优于聚合物；传统聚合物依托共价键结构，性能稳定、无动态重构特性。本文明确二者类比的适用边界与性能差异，为两类软物质材料的精准应用与理论完善提供支撑。 Wormlike micelles （WLMs） are self-assembled, one-dimensional aggregates that form transient entangled networks in aqueous solutions, exhibiting macroscopic rheology akin to that of traditional water-soluble polymers. This behavioral resemblance has fostered the “living polymer” analogy, yet the underlying assembly mechanisms differ fundamentally—a source of persistent ambiguity in both theory and application. To clarify these similarities and distinctions, a comparative study was reviewed on C22-tailed ultra-long zwitterionic EDAB wormlike micelles and ultra-high molecular weight （12×106 g/mol） polyacrylamide （PAM） using macroscopic rheology, small-angle neutron scattering, cryo-transmission electron microscopy, and reptation theory. Both systems behave as semi-flexible chains that form entangling networks above a critical overlap concentration, display shear thinning at high rates, and follow Arrhenius temperature dependence （25-85 ℃）, with optimal zero-shear viscosity reaching 40 000 mPa·s. Despite these parallels, their internal assembly modes diverge fundamentally. Traditional polymers rely on permanent covalent bonds—fixed chain length, molecular weight, and single reptation relaxation. In contrast, WLMs arise from reversible non-covalent hydrophobic and electrostatic interactions, yielding a thermodynamically balanced dynamic system with continuous breakage-reorganization and molecular exchange. Consequently, EDAB micelles exhibit 2.5-fold higher thickening efficiency and a lower critical overlap concentration （0.04 wt%） than PAM. They also show markedly higher temperature sensitivity （flow activation energy: 145 kJ/mol for EDAB vs. 24 kJ/mol for PAM）. While polymers display a multi-mode relaxation spectrum, WLMs obey Maxwell single-relaxation behavior at elevated temperatures and feature a dual relaxation mechanism coupling breakage with reptation. Morphologically, WLMs possess a larger core radius （~2.9 nm） and persistence length （≥20 nm）, with temperature-induced reversible transitions （shortening, branching, cyclization） ; polymer structures remain stable. Thus, the similarity between wormlike micelles and polymer solutions is only superficial under low concentrations and mild conditions; their essential dynamic and thermodynamic differences govern performance under complex environments. This analysis delineates the valid scope of the “living polymer” analogy and offers a theoretical basis for targeted design and application of both rheology modifiers. 数据和表 | 参考文献 | 相关文章 | 计量指标

基础研究

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压力对CO2泡沫性能及岩心流动影响 Effects of pressure on CO2 foam properties and core flow characteristics 郭程飞, 章海宁, 雷梦 2026 (5):  564-570.  doi: 10.3969/j.issn.2097-2806.2026.05.002 摘要 ( 77 )   HTML ( 6 )   PDF(1928KB) ( 72 )   针对压力对CO2泡沫性能及渗流特征影响不明确的问题，采用实验方法开展压力对CO2泡沫发泡性、稳定性、气泡直径、封堵能力、驱油效果研究。结果表明，压力越高CO2泡沫体系的发泡能力越强，压力对泡沫稳定性的影响存在竞争机制，纳米SiO2强化泡沫体系能够有效改善稳定性不足的问题；压力与气泡直径呈负相关关系，高压条件下形成的气泡直径小、分布范围集中；压力越高越有利于岩心中形成均一性好、运移过程稳定的CO2泡沫；压力越高CO2泡沫有效黏度越大、封堵能力越强，对储层渗透率的适用范围越宽；高压条件对CO2泡沫驱油效果具有促进作用，有利于改善储层非均质性带来的开发矛盾，实现大幅度提高采收率。研究结果深化了压力对CO2泡沫驱提高采收率认识，为现场实施CO2气窜治理提供参考。 Since the influences of pressure on the performance and seepage characteristics of CO2 foam are unclear, in this work, the effects of pressure on the foaming capability, stability, bubble diameter, plugging capacity, and oil displacement efficiency of CO2 foam were systematically investigated through experimental methods. The results showed that, the higher the pressure, the higher the foaming capacity of the CO2 foam system, however in contrast, there was a competitive mechanism in the effect of pressure on foam stability. The nano-SiO2-reinforced foam system effectively addressed the problem of instability in traditional foams. Negative correlation was observed between pressure and bubble diameter, with high-pressure conditions producing smaller and more uniform bubbles. Elevated pressure could promote the formation of CO2 foam with good uniformity and stable migration in cores. Moreover, the increased pressure could enhance the effective viscosity and plugging efficiency of CO2 foam, expanding its applicability to reservoirs with diverse ranges of permeability. High-pressure conditions could improve the oil displacement efficiency of CO2 foam flooding, alleviating the disadvantages situation caused by reservoir heterogeneity and achieving substantial oil recovery enhancement. This research might deepen the understanding of the role of pressure in CO2 foam-enhanced oil recovery and provide theoretical basis for effective gas channeling control strategies. 数据和表 | 参考文献 | 相关文章 | 计量指标

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季铵盐阳离子表面活性剂的制备及对织物抗菌及整理性能的影响研究 Study on the preparation of a quaternary ammonium surfactant and its effects on fabric antibacterial and finishing properties 方娇, 邢博 2026 (5):  571-579.  doi: 10.3969/j.issn.2097-2806.2026.05.003 摘要 ( 90 )   HTML ( 12 )   PDF(2632KB) ( 90 )   通过一步亲核取代反应成功合成了一种季铵盐阳离子表面活性剂（C16-GPBCH），产率达89%，采用核磁共振氢谱与傅里叶变换红外光谱对产物分子结构进行了表征，确认其高纯度与预期结构一致。在此基础上，采用浸渍法将C16-GPBCH应用于棉针织物功能整理，考察了整理液质量浓度、水浴温度及pH值等参数对织物抗静电性能与抗菌性能的调控规律。研究结果表明：当整理液质量浓度为30 g/L、水浴温度为70 ℃、pH=8时，整理后织物表现出最优的综合性能：其静电半衰期显著缩短至12.8 s，峰值电压降至912 V，与未整理织物相比，半衰期和峰值电压分别大幅下降79.5%与40.1%；同时对大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）的抑菌率分别达到99.95%和99.99%，并呈现明显抑菌圈，显示出优异的接触杀菌与扩散杀菌双重机制，该研究为构建高效和多功能整合的棉织物整理工艺提供了重要的实验依据和理论支持。 A cationic surfactant, i.e., a quaternary ammonium salt namely C16-GPBCH, was synthesized through a one-step nucleophilic substitution reaction, with the yield of 89%. The molecular structure of the product was characterized by 1H NMR and Fourier transform infrared spectroscopy, confirming its high purity and consistency with the expected structure. Then, C16-GPBCH was applied to the functional finishing of cotton knitted fabrics by impregnation method. The influences of those process parameters such as the mass concentration of the finishing solution, water bath temperature, and the pH upon the antistatic and antibacterial properties of fabrics were investigated. The results showed that, when the mass concentration of the finishing solution was 30 g/L, the water bath temperature was 70 ℃, and the pH was 8, the finished fabric exhibited the best comprehensive performance: The static half-life was significantly shortened to 12.8 s, and the peak voltage was reduced to 912 V. Compared with the untreated fabric, the half-life and peak voltage were decreased by 79.5% and 40.1%, respectively. In addition, the antibacterial rates against Escherichia coli （E. coli） and Staphylococcus aureus （S. aureus） reached 99.95% and 99.99%, respectively, indicative of obvious inhibition zones, which demonstrated excellent dual antibacterial mechanisms of contact killing and diffusion killing. This work might provide important experimental basis and theoretical support for constructing an efficient and multifunctional finishing process for cotton fabrics. 数据和表 | 参考文献 | 相关文章 | 计量指标

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氮化碳异质结催化剂的制备及光催化还原CO2性能研究 Preparation of carbon nitride heterojunction catalyst and its performance for photocatalytic CO2 reduction 高巍, 乔建芬, 郭雅娟 2026 (5):  580-588.  doi: 10.3969/j.issn.2097-2806.2026.05.004 摘要 ( 57 )   HTML ( 6 )   PDF(1618KB) ( 71 )   氮化碳是光催化还原CO2的一类理想催化剂，但光电子-空穴的重组极大地限制了氮化碳的应用。基于构建异质结策略，以三聚氰胺和3-氨基-1, 2, 4-三唑为原料，采用高温热聚合-热回流法合成了石墨相氮化碳/富氮氮化碳（C3N4/C3N5）催化剂，通过一系列手段对催化剂进行了表征。在300 W氙灯照射条件下向水相中通入高纯CO2进行光催化实验，评价C3N4/C3N5催化剂光催化还原CO2性能。结果表明，光照5 h，C3N4/C3N5催化剂光催化还原CO2只生成了CH4一种产物，产量达到了2.102 8 μmol/（g·h），是单组分C3N4和C3N5催化剂的9.8倍和5.2倍，光催化性能得到显著提升。一方面得益于C3N4和C3N5无序堆叠后比表面积和孔径的提升，另一方面得益于C3N4和C3N5耦合后Z型异质结的构建有效促进了光电子-空穴的分离，促进了电荷的转移。光催化CO2还原过程中光电子和空穴等活性物种起到关键作用。 Carbon nitride is an ideal catalyst for photocatalytic reduction of CO2, but the recombination of photoelectron-holes has greatly limited the application of carbon nitride. Based on the strategy of heterojunction construction, graphite-phase carbon nitride/nitrogen-rich carbon nitride （C3N4/C3N5） catalysts were synthesized by high-temperature thermal polymerization-thermal reflux method using melamine and 3-amino-1, 2, 4-triazole as raw materials. The catalysts were characterized by a series of methods. The photocatalytic performance of C3N4/C3N5 catalyst for CO2 reduction was evaluated by introducing high-purity CO2 into the aqueous phase under 300 W xenon lamp irradiation. The results showed that only CH4 was produced during the photocatalytic reduction of CO2 by C3N4/C3N5 catalyst under light irradiation for 5 h, and the yield reached 2.102 8 μmol/ （g·h）, which was 9.8 times and 5.2 times that of single-component C3N4 and C3N5 catalysts, respectively. The photocatalytic performance was significantly improved. It was not only due to the increases of specific surface area and pore size after the disordered stacking of C3N4 and C3N5, but also due to the construction of Z-scheme heterojunction after the coupling of C3N4 and C3N5, which effectively promoted the separation of photoelectron-holes and promoted the transfer of charges. Active species such as photoelectrons and holes played a key role in the process of photocatalytic CO2 reduction. 数据和表 | 参考文献 | 相关文章 | 计量指标

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偶联剂改性聚酰亚胺复合材料的制备及介电性能研究 Research of preparation and dielectric properties of polyimide composites modified by coupling agents 毛淑佳, 许树艳 2026 (5):  589-594.  doi: 10.3969/j.issn.2097-2806.2026.05.005 摘要 ( 63 )   HTML ( 3 )   PDF(1621KB) ( 76 )   选择 γ -甲基丙烯酰氧基丙基三甲氧基硅烷（KH570）作为硅烷偶联剂，制备出不同占比偶联剂KH570的复合石墨/聚酰亚胺胶粘剂体系，通过对复合体系的形貌、力学性能、热稳定性参数进行测试分析，结果表明：不同偶联剂KH570掺杂石墨的聚酰亚胺复合材料均为聚酰亚胺的结构。复合胶粘剂的剪切强度随偶联剂掺量增加先上升后下降，5%掺杂的复合体系剪切强度最高为27.93 MPa。偶联剂掺杂量越多，石墨/聚酰亚胺材料的介电常数增大，介电损耗降低，偶联剂掺量为7%的石墨/聚酰亚胺的介电常数最大为12.1，5%偶联剂掺杂量的石墨/聚酰亚胺介电损耗最低为0.018。偶联剂促进了聚酰亚胺复合体系的界面均匀性，提高了复合材料的介电性能和体系的热稳定性，所有复合胶粘剂的玻璃化转变温度基本都在261 ℃附近。 γ-Methacryloyloxypropyltrimethoxysilane （KH570） was selected as the silane coupling agent to prepare graphite/polyimide composites with different proportions of coupling agent KH570. The morphology, mechanical properties and thermal stability parameters of the composite system were tested and analyzed. The results showed that all the polyimide composites doped with graphite with different dosages of coupling agent KH570 exhibited the structure of polyimide. The shear strength of the composite adhesive first increased and then decreased with the increase of the dosage of coupling agent, among which the composite system with 5% coupling agent had the highest shear strength of 27.93 MPa. The higher the amount of coupling agent doping, the higher the dielectric constant and the lower the dielectric loss for the graphite/polyimide composite. In the investigated range, the dielectric constant of graphite/polyimide composite reached a maximum of 12.1 at the coupling agent content of 7%, while the minimum dielectric loss of 0.018 appeared at 5% coupling agent doping in the graphite/polyimide composite. The coupling agent improved the interface uniformity of the composite system, as well as the dielectric properties and the thermal stability thereof. The glass transition temperatures of all composite adhesives were around 261 ℃. 数据和表 | 参考文献 | 相关文章 | 计量指标

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超临界CO2萃取侧柏叶提取物对雄激素性脱发的防治功效及机制研究 Study on the efficacy and mechanism of Platycladus orientalis extracted by supercritical CO2 in prevention and treatment of androgenetic alopecia 冯艳菊, 董泓妍, 孟圆, 张誉荠, 袁旻嘉, 李从严 2026 (5):  595-602.  doi: 10.3969/j.issn.2097-2806.2026.05.006 摘要 ( 175 )   HTML ( 12 )   PDF(2275KB) ( 142 )   本研究旨在探究超临界CO2萃取侧柏叶提取物（SC-CO2-PO）的防脱发作用机制。采用超临界CO2萃取技术制备侧柏叶提取物，通过气相色谱-质谱联用技术（GC-MS）进行全成分解析及特征活性成分定量分析，建立基于AI的靶点预测模型筛选防脱关键靶点，采用MTT法测定SC-CO2-PO对人皮脂腺细胞（SZ95）的毒性作用，采用尼罗红荧光染色评价其对中性脂质抑制效果，采用酶活实验检测对II型5α-还原酶（SRD5A2）的抑制率，采用半定量RT-PCR检测雄激素受体（AR） mRNA相对表达水平，通过离体毛囊模型观察毛囊生长周期调控效应。结果表明，SC-CO2-PO含25种活性成分，其特征性活性成分雪松醇和β-石竹烯质量分数分别1.32%和0.10%；SC-CO2-PO在SZ95中的安全质量浓度为0.019 5 mg/mL；SC-CO2-PO能抑制中性脂质合成，抑制SRD5A2活性，减少AR mRNA相对表达含量，上调血管内皮生长因子（VEGF）表达水平和增加毛囊长度。这些结果表明，SC-CO2-PO能通过双途径起到防脱发作用。SC-CO2-PO通过抑制SRD5A2活性和下调AR mRNA相对表达水平，调控雄激素信号通路来减少皮脂分泌；SC-CO2-PO上调VEGF表达水平，在毛囊周期中促使毛囊周围血管重建，提高毛囊活性，促进头发的生长，延长毛囊生长期。 The aim of this study is to investigate the anti-hair loss mechanism of supercritical CO2 extraction of Platycladus orientalis （L.） Franco （SC-CO2-PO）. Supercritical CO2 extraction technology was used to prepare the extract of Platycladus orientalis （L.） Franco. Full component analysis and quantification of the characteristic active ingredients were performed by gas chromatography-mass spectrometry （GC-MS）. An AI-based target prediction model was established to screen key anti-shedding targets. The cytotoxic effect of SC-CO2-PO on human sebaceous gland cells （SZ95） was determined by MTT assay. Nile red fluorescence staining was used to evaluate its inhibitory effect on lipid synthesis. Enzyme activity assays were used to measure the inhibition rate of type II 5α-reductase （SRD5A2）. Semi-quantitative RT-PCR was used to detect the relative expression levels of androgen receptor （AR） mRNA. An in vitro hair follicle culture model was used to observe its regulatory effect on the hair follicle growth cycle. The results show that SC-CO2-PO contains 25 active ingredients, with the contents of the characteristic active ingredients cedarol and β-caryophyllene being 1.32% and 0.10%, respectively. The safe concentration of SC-CO2-PO in SZ95 cells is 0.019 5 mg/mL. SC-CO2-PO can inhibit neutral lipid synthesis, suppress SRD5A2 activity, reduce the relative expression levels of AR mRNA, increase hair follicle length and vascular endothelial growth factor （VEGF） content. These results indicate that SC-CO2-PO exerts anti-hair loss effects via dual pathways: it reduces sebum secretion by inhibiting SRD5A2 activity and down-regulating AR mRNA expression, thereby modulating the androgen signaling pathway. SC-CO2-PO increases the expression level of VEGF, promoting the reconstruction of blood vessels around hair follicles during the hair follicle cycle, enhancing the activity of hair follicles, stimulating hair growth, and prolonging the growth period of hair follicles. 数据和表 | 参考文献 | 相关文章 | 计量指标

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基于Nrf2/TLR4信号通路的低聚木糖皮肤防护功效原料开发、功效及机制研究 Research on development, efficacy and mechanism of xylooligosaccharides as a skin protective functional ingredient based on the Nrf2/TLR4 signaling pathway 张瑞敏, 张磊, 何亚飞, 范丽敏 2026 (5):  603-613.  doi: 10.3969/j.issn.2097-2806.2026.05.007 摘要 ( 84 )   HTML ( 4 )   PDF(1918KB) ( 61 )   研究低聚木糖（XOS）对皮肤屏障免疫功能的调控作用及分子机制，通过构建紫外线B（UVB）诱导损伤与金黄色葡萄球菌培养上清液（SA-CS）诱导炎症两种HaCaT细胞模型开展系统研究。UVB损伤模型中，HaCaT细胞经30 mJ/cm2 UVB照射后使用XOS低、中、高剂量（50，100，200 μg/mL）及Nrf2抑制剂ML385处理24 h；SA-CS炎症模型中，细胞经20% SA-CS处理24 h，同时在培养基中添加XOS低、中、高剂量及TLR4激动剂（LPS），每组均设6个复孔。检测细胞存活率、凋亡率（TUNEL法）、凋亡相关基因（Bax、Bcl-2） mRNA水平、跨膜电阻（TEER）值、屏障相关蛋白（FLG、Claudin-1/4） mRNA水平、氧化应激指标（ROS、MDA、SOD）、Nrf2/Keap1通路蛋白、炎症因子（IL-1β、IL-8、TNF-α等）及TLR4/NF-κB通路蛋白水平。结果显示：UVB照射显著降低HaCaT细胞存活率与TEER值，下调屏障相关蛋白mRNA表达，促进细胞凋亡，引发氧化应激并抑制Nrf2核转位；XOS以剂量依赖性方式逆转上述损伤，且该效应可被ML385阻断。SA-CS处理则显著降低细胞存活率与TEER值，下调屏障相关蛋白mRNA表达，促进细胞凋亡，升高炎症因子水平及TLR4、p-NF-κB p65蛋白表达；XOS同样以剂量依赖性方式改善上述损伤，而LPS可逆转其作用。本研究XOS通过激活Nrf2通路拮抗UVB诱导的皮肤细胞氧化损伤，通过抑制TLR4/NF-κB通路缓解SA-CS介导的炎症损伤，进而保护皮肤屏障功能。本研究为开发具有皮肤屏障保护、抗氧化及抗炎功效的化妆品原料的开发提供了实验依据与理论支撑。 To investigate the regulatory effect and molecular mechanism of xylooligosaccharides （XOS） on skin barrier immune function, we systematically evaluated two HaCaT cell models: ultraviolet B （UVB） -induced injury and Staphylococcus aureus culture supernatant （SA-CS） -induced inflammation. In the UVB injury model, HaCaT cells were irradiated with 30 mJ/cm2 UVB and then treated with low, medium and high doses of XOS （50, 100, 200 μg/mL） and an Nrf2 inhibitor （ML385） for 24 h. In the SA-CS inflammation model, cells were treated with 20% SA-CS for 24 h, with low, medium and high doses of XOS and a TLR4 agonist （LPS） added simultaneously. Each group consisted of six replicate wells. Cell viability, apoptosis rate （TUNEL assay）, mRNA levels of apoptosis-related genes （Bax, Bcl-2）, transepithelial electrical resistance （TEER） value, mRNA levels of barrier-related proteins （FLG, Claudin-1/4）, oxidative stress indicators （ROS, MDA, SOD）, proteins of the Nrf2/Keap1 pathway, inflammatory factors （IL-1β, IL-8, TNF-α, etc.） and protein levels of the TLR4/NF-κB pathway were measured. The results show that UVB irradiation significantly decreases cell viability and TEER value, downregulates mRNA expression of barrier-related proteins, promotes cell apoptosis, induces oxidative stress and inhibits Nrf2 nuclear translocation in HaCaT cells. XOS reverses these injuries in a dose-dependent manner, an effect that can be blocked by ML385. SA-CS treatment significantly reduces cell viability and TEER value, downregulates mRNA expression of barrier-related proteins, promotes cell apoptosis, and increases the levels of inflammatory factors and protein expression of TLR4 and p-NF-κB p65. XOS also ameliorates these injuries in a dose-dependent manner, while LPS reverses this effect. In summary, XOS antagonizes UVB-induced oxidative damage in skin cells by activating the Nrf2 pathway, and alleviates SA-CS-mediated inflammatory damage by inhibiting the TLR4/NF-κB pathway, thereby protecting skin barrier function. This study provides experimental evidence and theoretical support for the development of cosmetic raw materials with skin barrier protection, antioxidant and anti-inflammatory effects. 数据和表 | 参考文献 | 相关文章 | 计量指标

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浙江红山茶籽油酶解产物抗D-gal诱导皮肤成纤维细胞衰老作用及机制研究 Anti-aging effects and mechanism of enzymatic hydrolysate of Camellia chekiangoleosa Hu seed oil against D-galactose-induced senescence in human skin fibroblasts 卢琦, 向文叠, 李广涛, 尹世鹏, 高宏旗, 余果 2026 (5):  614-622.  doi: 10.3969/j.issn.2097-2806.2026.05.008 摘要 ( 94 )   HTML ( 7 )   PDF(2922KB) ( 90 )   探讨浙江红山茶籽油酶解产物（enzymatic hydrolysis products of Camellia chekiangoleosa Hu seed oil, EHCO）对D-gal诱导人皮肤成纤维细胞（human normal foreskin fibroblasts, HFF）衰老的保护作用及机制研究。使用60 mg/mL D-gal建立HFF细胞衰老模型，D-gal和体积分数0.1%，0.5% EHCO共同处理HFF细胞24 h，检测细胞活力和衰老水平。ELISA试剂盒检测ATP和SOD含量，ROS、JC-1、EDU荧光染色检测细胞线粒体功能和增殖水平，RT-qPCR检测衰老和炎症相关基因的表达，Western blotting检测凋亡和细胞自噬相关蛋白的表达。EHCO处理的HFF细胞内P16、P21、P53、IL-1β、IL-6 mRNA水平以及P53、ERK的磷酸化水平呈剂量依赖性降低（P<0.05），同时BAX与BCL-2蛋白比值减小，自噬相关蛋白（LC3B）表达升高。本研究发现生物脂肪酶切技术对红山茶籽油进行酶解获得的EHCO能够促进细胞增殖，对抗D-gal诱导的HFF细胞衰老。机制上，EHCO通过ERK-P53信号轴和激活细胞自噬改善成纤维细胞功能，来发挥抗皮肤衰老的作用。 This study investigated the protective effects and underlying mechanisms of the enzymatic hydrolysate from Camellia chekiangoleosa Hu seed oil （EHCO） on D-galactose （D-gal）- induced senescence in human normal foreskin fibroblasts （HFF）. A cellular senescence model was established by treating HFFs with 60 mg/mL D-gal. Cells were co-treated with D-gal and EHCO （0.1% or 0.5%） for 24 hours, and cell viability and senescence levels were subsequently assessed. The content of ATP and the activity of SOD were measured using ELISA kits. Mitochondrial function and proliferative capacity were evaluated via fluorescent staining for ROS, JC-1, and EDU. The mRNA expression levels of senescence-associated and inflammation-related genes were quantified by RT-qPCR. Furthermore, the protein expression of apoptosis-related and autophagy-related markers, along with the phosphorylation status of P53 and ERK, were analyzed by Western blotting. In EHCO-treated HFF, the mRNA levels of P16, P21, P53, IL-1β, and IL-6, as well as the phosphorylation levels of P53 and ERK, significantly reduce in a dose-dependent manner （P<0.05）. Concurrently, the pro-apoptotic BAX/BCL-2 protein ratio decreases, while the expression of the autophagy marker protein LC3B elevates. This study demonstrates that EHCO, obtained through lipase-catalyzed hydrolysis of Camellia chekiangoleosa Hu, promotes cell proliferation and protects against D-gal-induced senescence in HFF. Mechanistically, EHCO exerts anti-skin-aging effects by improving fibroblast function via the suppression of the ERK-P53 signaling axis and the activation of cellular autophagy. 数据和表 | 参考文献 | 相关文章 | 计量指标

开发与应用

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侧柏枝挥发油促进雄激素性脱发小鼠毛发生长研究 Study on essential oil from Platycladus orientalis L. branches for promoting hair growth in androgenetic alopecia mice 张蕾, 贺子涵, 杨俊, 周小魏, 王振华, 李刚, 马成俊 2026 (5):  623-634.  doi: 10.3969/j.issn.2097-2806.2026.05.009 摘要 ( 116 )   HTML ( 6 )   PDF(5661KB) ( 102 )   通过超临界CO2萃取技术从侧柏枝中提取挥发油，采用Box-Behnken响应面法优化萃取工艺，以萃取率为评价指标，利用气相色谱质谱联用技术对侧柏枝挥发油化学成分进行了表征。通过去除C57BL/6小鼠背部毛发及腹腔注射睾酮建立雄激素性脱发模型，评价侧柏枝挥发油促进毛发生长的效果。通过网络药理学研究侧柏枝挥发油促进毛发生长的机制，使用ELISA试剂盒检测小鼠皮肤中IL-1β，IL-6，TNF-α，SOD，CAT，MDA水平。结果表明，侧柏枝挥发油最优萃取工艺为压力10.62 MPa，温度43.69 ℃，萃取时间83.86 min，最大萃取率为20.107%，该挥发油的主要成分包括罗汉柏烯（含量32.27%）和雪松醇（含量20.88%）。在实验第21天，10%侧柏枝挥发油处理组小鼠实现了完全毛发生长，效果与米诺地尔对照组相当，组织学分析显示，侧柏枝挥发油能提高毛囊密度，并加速毛囊向生长期转化。网络药理学分析显示侧柏枝挥发油参与炎症和氧化应激，侧柏枝挥发油能有效抑制炎症因子IL-1β，IL-6，TNF-α，同时恢复抗氧化酶（SOD和CAT）活性并减轻脂质过氧化反应（MDA水平）。该研究为侧柏挥发油在护发产品中的应用提供参考，为雄激素性脱发患者提供更多选择。 Supercritical CO2 extraction was employed to isolate the essential oil from Platycladus orientalis L. branches （EOPB）. The extraction process was optimized using a Box-Behnken design （BBD） with the extraction yield as the response variable. The chemical constituents of the extracted essential oil were characterized via gas chromatography-mass spectrometry. An androgenetic alopecia （AGA） model was established in C57BL/6 mice by depilating the dorsal hair and administering daily intraperitoneal injections of testosterone to evaluate the hair growth-promoting effects of EOPB. The underlying mechanisms were investigated using network pharmacology. Furthermore, the cutaneous levels of IL-1β, IL-6, TNF-α, SOD, CAT, and MDA were measured using ELISA kits. The results indicate that the optimal extraction parameters for EOPB are a pressure of 10.62 MPa, a temperature of 43.69 ℃, and an extraction time of 83.86 minutes, achieving a maximum extraction yield of 20.107%. The primary constituents of EOPB are identified as thujopsene （32.27%） and cedrol （20.88%）. The 10% （φ/%） EOPB group achieves complete hair regrowth by day 21, exhibiting an efficacy comparable to that of the minoxidil positive control. Histological analysis confirms that EOPB enhances hair follicle density and accelerates the anagen phase transition. Network pharmacology analysis reveals the involvement of EOPB in inflammatory and oxidative stress pathways. EOPB effectively suppresses inflammatory factors （IL-1β, IL-6, TNF-α）, restores antioxidant enzyme （SOD and CAT） activities, and reduces lipid peroxidation （MDA levels）. This study provides a reference for the application of EOPB in hair care products, offering alternative therapeutic choices for patients with androgenetic alopecia. 数据和表 | 参考文献 | 相关文章 | 计量指标

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光甘草定的美白作用及其机制 Whitening effect of glabridin and its mechanism 梁君, 石晓明 2026 (5):  635-643.  doi: 10.3969/j.issn.2097-2806.2026.05.010 摘要 ( 139 )   HTML ( 6 )   PDF(3727KB) ( 114 )   本研究旨在揭示光甘草定（glabridin，GLA）的美白作用及其机制。以紫外线B（UVB）诱导的皮肤光老化（SP）小鼠为动物模型。将小鼠随机分配至6个处理组（n=12），分别为对照组（C组）、UVB组、低、中、高剂量光甘草定组（L、M、H-GLA）、高剂量光甘草定+核因子E2相关因子2（Nrf2）抑制剂ML385组（H-GLA+ML385）。C组常规饲养且不接受UVB辐照，其余各组均接受UVB辐照处理。各组于紫外线暴露前1 h进行经皮给药。每天给药1次，共给药8周。采用苏木素伊红（HE）染色与Masson三色染色进行皮肤组织形态学分析。采用NaOH溶解法测定黑色素含量。ELISA法检测酪氨酸酶活性。使用商业试剂盒分别测定超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）和丙二醛（MDA）含量。通过二氢乙锭（DHE）染色进行活性氧（ROS）检测。使用ELISA法检测皮肤组织白介素（IL） -6、肿瘤坏死因子-α （TNF-α）和IL-1β水平。RT-qPCT检测I型胶原α1链（COL1A1）、基质金属蛋白酶-1 （MMP-1）、酪氨酸酶相关蛋白 （TRP） -1、TRP-2、血红素加氧酶-1 （HO-1） 和NAD （P） H：醌氧化还原酶-1 （NQO-1） mRNA水平。Western blot检测皮肤α-MSH、Nrf2 （细胞核）和Keap1蛋白表达。结果显示，与UVB组比较，3个剂量的光甘草定（L、M和H-GLA组）减轻了UVB诱导的皮肤光老化小鼠皮肤损伤和胶原变性，降低了MMP-1 mRNA水平，升高了COL1A1 mRNA水平，降低了皮肤黑色素含量、酪氨酸酶活性、TRP-1和TRP-2 mRNA相对水平、α-MSH蛋白相对表达量，升高了皮肤SOD、CAT和GSH-Px活性，降低了MDA含量和ROS相对荧光强度，降低了皮肤IL-6、TNF-α和IL-1β含量，升高了Nrf2（细胞核）蛋白表达量、HO-1和NQO-1 mRNA相对表达量，降低了Keap1蛋白相对水平（P<0.05）。然而，Nrf2抑制剂ML385减弱了光甘草定的上述影响（P<0.05）。结果表明，光甘草定通过激活Nrf2减轻UVB诱导的皮肤光老化小鼠皮肤损伤和胶原变性，抑制黑色素生成，减轻氧化应激和炎症。 This study aimed to investigate the whitening effect of glabridin （GLA） and its mechanism. A mouse model of ultraviolet B （UVB） -induced skin photoaging （SP） mice was used. The mice were randomly assigned to 6 treatment groups （n=12）, which were control group （C group）, UVB group, low, medium and high dose glabridin group （L, M, H-GLA）, and high dose glabridin + nuclear factor E2-related factor 2 （Nrf2） inhibitor ML385 group （H-GLA+ML385）. Group C was conventionally fed without UVB irradiation, while the other groups were subjected to UVB irradiation. Each group received percutaneous administration 1 h before UV exposure once daily for 8 weeks. The skin was analyzed by hematoxylin and eosin （HE） staining and Masson’s trichrome staining. Melanin content was determined by the NaOH dissolution method. Tyrosinase activity was detected by ELISA. The activities of superoxide dismutase （SOD）, catalase （CAT）, and glutathione peroxidase （GSH-Px） and the content of malondialdehyde （MDA） were determined using commercial kits. Reactive oxygen species （ROS） were detected by dihydroethidium （DHE） staining. The levels of interleukin-6, tumor necrosis factor-α （TNF-α） and IL-1β in skin tissue were detected by ELISA. mRNA levels of type I collagen α1 chain （COL1A1）, matrix metalloproteinase-1 （MMP-1）, tyrosinase-associated protein （TRP） -1, TRP-2, heme oxygenase-1 （HO-1） and NAD （P） H: quinone oxidoreductase-1 （NQO-1） were detected by RT-qPCT. Western blot was used to analyze protein expression of α-MSH, Nrf2 （nuclear） and Keap1 in the skin. The results show that compared with the UVB group, the three doses of glabridin （L, M and H-GLA groups） alleviate UVB-induced skin damage and collagen degeneration in the skin photoaging mice, decrease the level of MMP-1 mRNA and increase the level of COL1A1 mRNA. decrease skin melanin content, tyrosinase activity, the levels of TRP-1 and TRP-2 mRNA, the relative expression level of α-MSH protein, increase the skin SOD, CAT and GSH-Px activities, decrease MDA content and ROS relative fluorescence intensity, and decrease skin IL-6, TNF-α and IL-1β contents, increase the expression level of Nrf2 （nuclear） protein, and HO-1 and NQO-1 mRNA, and decrease the relative expression level of Keap1 protein （P<0.05）. However, the Nrf2 inhibitor ML385 attenuates the above effects of glabridin （P<0.05）. These results indicate that glabridin alleviates UVB-induced skin damage and collagen degeneration, inhibits melanin production, and reduces oxidative stress and inflammation in mice with skin photoaging by activating Nrf2. 数据和表 | 参考文献 | 相关文章 | 计量指标

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啤特果发酵滤液的多通路抗衰功效研究 A study on the anti-aging mechanism of Piteguo fermentation filtrate 聂梦琳, 王冠凤, 阚洪玲, 席清源, 张羽辰, 陈嘉慧 2026 (5):  644-653.  doi: 10.3969/j.issn.2097-2806.2026.05.011 摘要 ( 81 )   HTML ( 5 )   PDF(2298KB) ( 63 )   采用葡萄汁有孢汉逊酵母（Hanseniaspora uvarum）标准化发酵工艺制备啤特果发酵滤液，并系统评价其多通路抗衰功效。发酵后啤特果滤液中的有机酸、苯丙素类及黄酮类化合物含量均显著提升，表明其具有通过抗氧化与抗炎通路协同发挥抗衰老作用的潜力。体外实验表明，该发酵滤液可通过以下机制协同发挥抗衰功效：清除自由基，降低细胞内氧化应激引起的活性氧生成；在炎症衰老模型中，抑制衰老相关-β-半乳糖苷酶活性及促炎因子分泌，改善衰老标志蛋白表达；减少细胞中糖基化终末产物积累，体现抗糖化潜力；显著上调 I 型胶原蛋白表达，干预皮肤衰老的核心环节。鸡胚绒毛尿囊膜试验与人体试验证实其外用安全无刺激性且无不良反应。随机对照人体试验表明长期使用可提升紧致度并减少皱纹。综上，啤特果发酵滤液通过抗氧化、抗炎症衰老及抗糖化，实现了胶原合成发挥抗皮肤衰老的作用，为啤特果高价值化开发及抗衰功效化妆品创新提供了理论与应用基础。 Using a standardized fermentation process with the yeast Hanseniaspora uvarum, Piteguo fermentation filtrate was produced, and its multi-pathway anti-aging efficacy was comprehensively assessed. After fermentation, the contents of organic acids, phenylpropyl compounds and flavonoids in the Piteguo filtrate were significantly increased, indicating its potential to exert anti-aging effects through synergistic antioxidant and anti-inflammatory pathways. In vitro experimental results reveal that Piteguo fermentation filtrate exerts synergistic anti-aging effects through several key mechanisms: it scavenges free radicals, reduces intracellular oxidative stress levels, and attenuates reactive oxygen species （ROS） generation. In an inflammageing model, it inhibits senescence-associated β-galactosidase （SA-β-gal） activity and the secretion of pro-inflammatory cytokines, while also improving the expression profile of key senescence marker proteins. It reduces the cellular accumulation of advanced glycation end products （AGEs）, indicating notable anti-glycation potential. It significantly upregulates the expression of type I collagen （COL I）, thereby directly intervening in a fundamental structural component of skin aging. Safety evaluations, including the Hen's Egg Test-Chorioallantoic Membrane （HET-CAM） assay and controlled human trials, show no skin irritation or adverse symptoms. A randomized controlled human efficacy study demonstrates that sustained use of Piteguo fermentation filtrate leads to measurable improvements in skin firmness and a reduction in wrinkle severity. In summary, Piteguo fermentation filtrate combats skin aging through a combined strategy involving antioxidant defense, suppression of inflammaging, inhibition of glycation, and stimulation of collagen synthesis. These findings provide a robust scientific foundation for the high-value utilization of this regional fruit and support its innovative application in anti-aging cosmetic formulations. 数据和表 | 参考文献 | 相关文章 | 计量指标

专论与综述

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渤海高含水油田化学法提高采收率技术研究进展及发展方向 Research progress and development directions of enhanced oil recovery technologies for Bohai high water-cut oilfields 郑玉飞, 亓翔, 李翔, 刘金龙, 覃庆波, 贾永康, 冯轩 2026 (5):  654-665.  doi: 10.3969/j.issn.2097-2806.2026.05.012 摘要 ( 67 )   HTML ( 2 )   PDF(4857KB) ( 82 )   随着部分海上老油田进入中高含水阶段，稳油控水已成为提高采收率技术的重要手段之一。本文概述了渤海高含水油田提高采收率技术发展历程、研究进展及现场应用情况，针对渤海油田地质油藏特征和水驱开发过程中的主要矛盾，归纳总结了适用的提高采收率技术种类，主要包括调剖体系、调驱体系和驱油体系三大类，系统介绍了各类技术的作用机理、研究现状和实践成果，分析了现有技术不足之处，并对渤海油田提高采收率技术面临主要挑战和未来发展方向进行了展望，即高温高压及低渗等非常规油田的开发是技术面对的主要难题，未来在化学药剂研发方面需更注重绿色环保、智能化、低成本及多功能新材料的研发，在施工工艺方面则应向规模化、在线化、自动化趋势发展。 As some offshore oil fields enter the medium to high water-cut stage, oil stabilization and water control have become one of the important means to enhance oil recovery. In this review, the development history, research progress, and field applications of chemical EOR technologies in Bohai oilfield were outlined. Addressing the reservoir geological characteristics of Bohai oilfield and the key challenges during waterflooding, those applicable chemical EOR technologies were summarized and primarily categorized into three systems: Profile control systems, profile control and flooding systems, and oil displacement systems. The operational mechanisms, research status, and practical achievements of each technology category were systematically introduced, and the existing technical limitations were also analyzed. Furthermore, the main challenges and future development directions for chemical EOR technologies in Bohai oilfield were prospected. Notably, the development of unconventional reservoirs featured by high temperature, high pressure, and low permeability was the major technical obstacle. Future research should prioritize the development of green and environmentally friendly, intelligent, low-cost, and multifunctional chemical materials. In terms of operational processes, the industry should advance toward large-scale implementation, online operations, and automated technologies. 数据和表 | 参考文献 | 相关文章 | 计量指标

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表面活性剂在农药多菌灵剂型研发中的应用进展 Advances of surfactants in the development of formulations of pesticide carbendazim 薛黎明, 徐佳乐, 周婧娴, 金玉娥 2026 (5):  666-673.  doi: 10.3969/j.issn.2097-2806.2026.05.013 摘要 ( 65 )   HTML ( 1 )   PDF(1665KB) ( 66 )   多菌灵是一种广谱高效杀菌剂，其传统剂型可湿性粉剂（WP）利用率低、污染高、易诱发抗药性。表面活性剂可提升药效、减少环境残留与施药者暴露风险。本文综述了表面活性剂在多菌灵悬浮剂（SC）、水分散粒剂（WG）及纳米制剂中的研究进展。阐述了多菌灵制剂中表面活性剂通过空间位阻与静电稳定机制提升制剂物理稳定性，通过增加溶解度、叶面附着与吸收作用来提升利用率，并分析了其潜在负面效应和绿色化设计策略以降低环境行为与职业暴露风险。指出未来开发高效、绿色可降解的表面活性剂，并融合纳米技术，是实现多菌灵农药绿色、可持续发展的重要方向。 Carbendazim is a broad-spectrum and highly effective benzimidazole fungicide. However, its conventional formulation, wettable powder （WP）, suffers from issues such as low utilization ratio, high environmental pollution, and a tendency to induce drug resistance. Surfactants are one of the key components in pesticide formulations, which have also been used in carbendazim formulations to address these traditional challenges. Surfactants can enhance efficacy and utilization, reduce environmental residues, and lower exposure risks for applicators. In this review, the research progress of surfactants in carbendazim formulations was summarized, including suspension concentrates （SC）, water-dispersible granules （WG）, and nano-formulations. It was elaborated that the surfactants in carbendazim formulations had improved physical stability through steric hindrance and electrostatic stabilization, and they could also enhance utilization by increasing solubility, foliar adhesion, and absorption. Then, potential negative effects and green design strategies to reduce environmental fate and operator exposure risks were discussed. It was pointed out that the future development of efficient, green and bio-degradable surfactants combined with nanotechnology would be a crucial direction for achieving green and sustainable development of carbendazim pesticides. 数据和表 | 参考文献 | 相关文章 | 计量指标

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国内外化妆品不良反应监管动态与企业实践 Cosmetovigilance: regulatory trends and corporate practices across countries 宁曼如, 梁艺怀, 王飞飞 2026 (5):  674-680.  doi: 10.3969/j.issn.2097-2806.2026.05.014 摘要 ( 77 )   HTML ( 4 )   PDF(2939KB) ( 77 )   化妆品上市后监管是产品全生命周期风险控制的重要环节，其中“化妆品警戒”作为核心手段，逐渐成为国内外监管体系中的关键组成。文章梳理了欧盟、美国、日本以及我国的化妆品不良反应监管法规与运行机制，并分析了国际知名企业在化妆品警戒领域的体系建设与实践经验，以期为我国化妆品积极响应监管要求、履行法规义务、完善内部化妆品警戒体系提供借鉴。 Post-marketing surveillance （PMS） is a critical component of risk management throughout the entire product lifecycle of cosmetics. As a core element of PMS, cosmetovigilance has emerged as a central approach and a key pillar of regulatory systems both domestically and internationally. This review summarizes the regulatory frameworks and operational mechanisms for cosmetovigilance in the European Union, the United States, Japan, and China. Furthermore, two international renowned cosmetic companies were selected as case studies to demonstrate how they developed their internal PMS systems and benefits derived from them. The aim is to provide insights and references for domestic cosmetic companies to actively respond to regulatory requirements, fulfill their legal obligations, and enhance their internal cosmetovigilance systems. 数据和表 | 参考文献 | 相关文章 | 计量指标

分析与检测

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高温气相色谱法测定LAB中PPO含量 Determination of PPO content in LAB by high-temperature gas chromatography 黄爱忠, 李映雪, 刘冬, 陈丽君, 董小宇, 曹红 2026 (5):  681-686.  doi: 10.3969/j.issn.2097-2806.2026.05.015 摘要 ( 65 )   HTML ( 2 )   PDF(1360KB) ( 65 )   采用高温气相色谱仪冷柱头进样结合外标定量法建立了以烷基苯（LAB）为主体介质的液体闪烁体中第一闪烁体2, 5-二苯基恶唑（PPO）含量的分析方法。试验结果表明，PPO在0~0.856 g/L区间内，相关系数R2高于0.99，线性关系良好。本方法的检出限为2.33 μg/L，定量限为7.00 μg/L，回收率为97.0%~100.1%。本方法操作简单，具备较高的灵敏度与准确性，结果稳定性好，检测数据可靠，适用于以LAB为主体介质的液体闪烁体中PPO含量的测定，为以LAB为主体介质的液体闪烁体质量控制提供了有力分析手段。 An analytical method for determining the content of primary scintillator 2, 5-diphenyloxazole （PPO） in liquid scintillators composed primarily of linear alkylbenzene （LAB） was established using a high-temperature gas chromatograph with a cold column inlet and an external standard quantification method. The results demonstrate excellent linearity for PPO in the concentration range of 0-0.856 g/L, with correlation coefficients R2 > 0.99. The limit of detection of the method is 2.33 μg/L, the limit of quantification is 7.00 μg/L, and the recovery rate ranges from 97.0% to 100.1%. This method is straightforward, high sensitivity and accurate, provides stable results, and yields reliable data. It is suitable for the quantitative analysis of PPO in LAB-based liquid scintillators, offering a robust analytical method for quality control of LAB-formulated liquid scintillation systems. 数据和表 | 参考文献 | 相关文章 | 计量指标

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高效液相色谱法检测人参化妆品制剂中6种人参皂苷含量 Determination of the contents of six ginsenosides in ginseng cosmetic preparations by high performance liquid chromatography 秦松, 李松宾 2026 (5):  687-694.  doi: 10.3969/j.issn.2097-2806.2026.05.016 摘要 ( 93 )   HTML ( 3 )   PDF(1269KB) ( 93 )   构建了一种基于高效液相色谱法（HPLC）的人参化妆品制剂中6种人参皂苷（Rb1、Rb2、Rc、Rd、Re、Rg1）的同步定量分析方法。选用80%乙醇溶液作为提取溶剂，通过石油醚脱脂协同水饱和正丁醇萃取技术消除基质干扰，结合控温浓缩处理保护热敏性组分。样品在40 ℃下超声萃取40 min（功率300 W，频率40 kHz），期间每10 min涡旋10 s。采用核壳结构Kinetex C18色谱柱（100 mm×2.1 mm, 2.6 μm），以乙腈-0.1%磷酸水为流动相实施梯度洗脱，在203 nm波长下实现35 min内6种皂苷的基线分离。方法学验证表明，日内精密度相对标准偏差（RSD）为1.72%~4.65%，日间精密度RSD为2.24%~4.59%，RSD均小于5%。线性回归分析显示，6种人参皂苷的相关系数（r）均大于0.999，检测限（LOD）范围为0.11~0.23 μg/mL，定量限（LOQ）范围为0.33~0.70 μg/mL。6种人参皂苷在不同加标质量浓度下的平均回收率范围为95.73%~104.65%，RSD均优于5%。本研究依据优化后的HPLC检测方法对市售10种人参类化妆品（涵盖精华液、面霜、乳液三类）中6种人参皂苷进行定量分析，结果表明Rb1为优势皂苷，膏霜总皂苷（均值471.0~552.7 μg/g）高于精华液（均值178.7~418.4 μg/g）和乳液（均值212.1~347.6 μg/g）。该方法首次构建三重净化-快速分离联用体系，显著提升检测效率并克服化妆品乳化体系干扰，为人参功效成分质量控制提供技术支撑。 In this study, a synchronous quantitative analytical method for six ginsenosides （Rb1, Rb2, Rc, Rd, Re, Rg1） in ginseng cosmetics using high performance liquid chromatography （HPLC） was established. The method uses 80% ethanol solution as the extraction solvent. Matrix interference is eliminated by degreasing with petroleum ether combined with extraction with water-saturated n-butyl alcohol, and the thermosensitive components are protected by temperature-controlled concentration. Samples were ultrasonically extracted at 40 ℃ for 40 min （power 300 W, frequency 40 kHz）, with vortexing for 10 s every 10 min. A Kinetex C18 chromatographic column with a core-shell structure （100 mm×2.1 mm, 2.6 μm） was used, and gradient elution was performed with acetonitrile-0.1% phosphoric acid aqueous solution as the mobile phase （the proportion of acetonitrile increases from 18% to 55% within 0-35 min）, which achieved baseline separation of the six ginsenosides within 35 min at a wavelength of 203 nm. Method validation shows that the relative standard deviation （RSD） of intra-day precision is 1.72%-4.65%, and the RSD of inter-day precision is 2.24%-4.59%, both less than 5%. Linear regression analysis shows that the correlation coefficients （r） of the six ginsenosides are all greater than 0.999, the limit of detection （LOD） ranges from 0.11 to 0.23 μg/mL, and the limit of quantification （LOQ） ranges from 0.33 to 0.70 μg/mL. The average recoveries of the six ginsenosides at different spiked mass concentrations range from 95.73% to 104.65%, with RSDs all less than 5%. Using this optimized HPLC method, this study quantitatively analyzes the six ginsenosides in 10 commercially available ginseng cosmetics （including essences, creams, and lotions）. The results show that Rb1 is the dominant ginsenoside, and the total ginsenoside content in creams （average 471.0-552.7 μg/g） is higher than that in essences （average 178.7-418.4 μg/g） and lotions （average 212.1-347.6 μg/g）. This method, which combines triple purification with rapid separation, significantly improves detection efficiency and overcomes interference from the cosmetic emulsion system, providing technical support for quality control of the functional components of ginseng. 数据和表 | 参考文献 | 相关文章 | 计量指标