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日用化学工业（中英文）

日用化学工业 ›› 2015, Vol. 45 ›› Issue (12): 715-718.doi: 10.13218/j.cnki.csdc.2015.12.012

• 分析与检测 • 上一篇    

高效液相色谱法同时测定洗发水中5种去屑剂

郭洁, 张华珺, 刘齐, 张蓉   

  1. 北京市药品检验所,北京 100035
  • 收稿日期:2015-10-13 修回日期:2015-11-24 发布日期:2019-04-10
  • 作者简介:郭 洁(1988-),女,北京人,硕士研究生,E-mail:cherylguo126@126.com。

Simultaneous determination of content of five kinds of anti-dandruff agent in shampoo by high performance liquid chromatography

GUO Jie, ZHANG Hua-jun, LIU Qi, ZHANG Rong   

  1. Beijing Institute For Drug Control,Beijing 100035,China
  • Received:2015-10-13 Revised:2015-11-24 Published:2019-04-10

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摘要： 建立了一种同时测定洗发水中水杨酸、吡硫翁锌、酮康唑、氯咪巴唑和吡罗克酮乙醇胺盐5种去屑剂的高效液相色谱法。采用C18柱,以乙腈-甲醇-0.01 mol/L的磷酸二氢钾溶液(含乙二胺四乙酸二钠0.5 mmol/L,磷酸调节pH=4)梯度洗脱分离各个组分,进样体积5 μL,柱温35 ℃,检测波长230 nm。结果表明:水杨酸、吡硫翁锌、酮康唑和氯咪巴唑的线性范围为10~200 mg/L,吡罗克酮乙醇胺盐的线性范围为20~400 mg/L,方法的线性关系良好。水杨酸、吡硫翁锌、酮康唑、氯咪巴唑和吡罗克酮乙醇胺盐的检出限分别为0.26,0.53,0.26,0.26和2.0 mg/L,平均回收率为96.04%~98.42%。采用该方法对83件市售去屑产品进行检测:有57件检出吡硫翁锌,18件检出吡罗克酮乙醇胺盐,7件检出水杨酸,3件检出氯咪巴唑,未检出禁用物质酮康唑。所有产品去屑剂含量均符合标准要求。

关键词: 洗发水, 去屑剂, 高效液相色谱法

Abstract: A high performance liquid chromatography method with gradient elution was established for simultaneous determination of anti-dandruff agent salicylic acid,pyrithione zinc,ketoconazole,climbazole and octopirox in shampoo formulations.A C18 column was used as stationary phase,and mixture containing acetonitrile-methanol aqueous solution (0.01 mol/L KH2PO4 and 0.5 mmol/L EDTA-2Na,pH adjusted to 4 with H3PO4) eluted by gradient was used as mobile phase in the HPLC at the wavelength of 230 nm.Operating temperature was 35 ℃.Injection volume was 5 μL.The linearity range of salicylic acid,pyrithione zinc,ketoconazole and climbazole is 10~200 mg/L.The linearity range of octopirox is 20~400 mg/L.The detection limits for salicylic acid,pyrithione zinc,ketoconazole,climbazole and octopirox are 0.26,0.53,0.26,0.26 and 2.0 mg/L respectively.The spiked recovery of matrix for salicylic acid,pyrithione zinc,ketoconazole,climbazole and octopirox varies from 96.04% to 98.42%.Among test results of 83 shampoo samples from market,pyrithione zinc appears in 57 samples;octopirox appears in 18 samples;salicylic acid appears in 7 samples;climbazole appears in 3 samples;banned substance ketoconazole is not detected.The contents of anti-dandruff agents in all samples meet the relevant standard requirements.

Key words: shampoo, anti-dandruff agent, high performance liquid chromatography

中图分类号: 

  • TQ658.3+3