广西珊瑚姜精油成分GC-MS分析及抑菌活性研究

doi:10.3969/j.issn.1001-1803.2021.06.011

摘要/Abstract

摘要：

以广西珊瑚姜精油为实验材料,测定该产区珊瑚姜精油的成分以及对巨大芽孢杆菌、球毛壳霉菌等8种菌的抑菌性能。采用气相色谱-质谱联用仪（GC-MS）对精油的成分进行测定并分析,通过纸片扩散法测定抑菌能力,利用试管稀释法测定最小杀菌浓度和最小抑菌浓度（MIC）。结果显示,该产区珊瑚姜精油中共检测出36种化学成分,其主要成分包括桧烯（27.87%）、松油烯-4-醇（22.57%）、1,4-双（甲氧基）-三戊并烯（10.67%）等,其它微量成分有萜品油烯、α-松油醇等;珊瑚姜精油对本实验中的8种菌的抑菌能力有差别,珊瑚姜精油对于细菌的抑制效果不如阳性药物,但对真菌的抑制效果优于阳性药物,其中,对大肠埃希氏菌、球毛壳霉菌、红色毛癣菌的抑制能力较强,MIC为1 µL/mL,对黄曲霉菌的抑制能力相对较弱,MIC为15 µL/mL,对巨大芽孢杆菌、金黄色葡萄球菌、普通变形杆菌、白色念珠菌的MIC为3 µL/mL。广西珊瑚姜精油对于实验用菌具有一定的抑菌性能且具备广谱性,有较好的开发前景。

关键词: 珊瑚姜, 精油, GC-MS分析, 成分, 抑菌活性

Abstract:

The essential oil of Guangxi Zingiber corallinum Hance was used as the experimental material to measure the components thereof. Its antibacterial/antifungal abilities against 8 kinds of bacteria and fungi were also studied, such as Bacillus megaterium and Chaetomium globosum. The components of Zingiber corallinum Hance essential oil was determined by gas chromatography-mass spectrometry （GC-MS）. Its inhibition zone was determined by disk diffusion method, and its minimum inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） or minimum fungicidal concentration （MFC） were determined by tube dilution method. The results show that there are 36 kinds of components detected in the Zingiber corallinum Hance essential oil from this region, and its main components include sabinene （27.87%）, terpinen-4-ol （22.57%） , triquinacene 1,4-bis（methoxy）-（10.67%） and so on. Other trace components include terpinolene, α-terpineol, etc. Zingiber corallinum Hance essential oil has different antibacterial/antifungal abilities against the eight kinds of bacteria and fungi in the experiment. Compared with the positive drug, Zingiber corallinum Hance essential oil has worse inhibitory effects against bacteria but has better inhibitory effects against fungi. The inhibitory effects against Escherichia coli, Chaetomium globosum and Trichophyton rubrum are stronger, with MIC of 1 µL/mL; the inhibitory effect against Aspergillus flavus is relatively weak, with MIC of 15 µL/mL. The MIC against Bacillus megaterium, Staphylococcus aureus, Proteus vulgaris and Candida albicans is 3 µL/mL. Guangxi Zingiber corallinum Hance essential oil has certain antibacterial performance and broad spectrum to experimental bacteria, and has a good development prospect.

Key words: Zingiber corallinum Hance, essential oil, GC-MS analysis, component, antimicrobial activity

中图分类号:

TQ658

程红柳,王文翠,姚雷,李玉红. 广西珊瑚姜精油成分GC-MS分析及抑菌活性研究[J]. 日用化学工业, 2021, 51(6): 539-545.

CHENG Hong-liu,WANG Wen-cui,YAO Lei,LI Yu-hong. GC-MS analysis and antibacterial/antifungal abilities of the essential oil of Guangxi Zingiber corallinum Hance[J]. China Surfactant Detergent & Cosmetics, 2021, 51(6): 539-545.

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参考文献 21

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精油含量/（μL·mL^-1）	V/μL
精油含量/（μL·mL^-1）	精油	丙酮	培养基	菌悬液
0.1	0.5	2.5	4 497	500
0.2	1	5	4 494	500
0.5	2.5	12.5	4 485	500
1	5	25	4 470	500
3	15	75	4 410	500
5	25	125	4 350	500
7	35	175	4 290	500
10	50	250	4 200	500
15	75	375	4 050	500
20	100	500	3 900	500
25	125	625	3 750	500
30	150	750	3 600	500
丙酮	0	750	3 600	500
培养基	0	0	3 600	500

分类	成分	保留时间/min	相对含量/%
烯类	桧烯（Sabinene）	11.881 8	27.871 0
	1,4-双（甲氧基）-三戊并烯（Triquinacene, 1,4-bis（methoxy）-）	50.202 7	10.673 5
	γ-松油烯（γ-Terpinene）	19.462 6	6.356 3
	2-蒈烯（2-Carene）	17.070 4	3.414 4
	β-倍半水芹烯（β-Sesquiphellandrene）	42.215 1	3.408 2
	β-蒎烯（β-Pinene）	10.517 4	2.908 6
	α-蒎烯（α-Pinene）	4.562 5	1.753 5
	β-月桂烯（β-Myrcene）	15.720 2	1.727 4
	β-水芹烯（β-Phellandrene）	18.014 6	1.538 4
	反式-桧烯氢氧化物（trans-Sabinene hydroxide）	31.259 1	1.507 8
	邻聚伞花素（o-Cymene）	20.212 7	1.344 3
	萜品油烯（Terpinolene）	20.774 2	1.283 8
	α-侧柏烯（α-Thujene）	4.950 2	1.216 6
	D-柠檬烯（D-Limonene）	17.665 8	0.657 8
	姜烯（Zingiberene）	39.104 7	0.493 1
	β-甜没药烯（β-Bisabolene）	39.477 1	0.196 9
	γ-榄香烯（γ-Elemene）	34.852 2	0.159 6
醇类	松油烯-4-醇（Terpinen-4-ol）	33.322 6	22.570 2
	顺式-芳樟醇氧化物（cis-Linalyl oxide）	28.105 7	1.216 8
	顺式-2-薄荷醇（cis-2-Menthenol）	31.786 2	1.194 0
	α-松油醇（α-Terpineol）	37.401 6	1.063 1
	反式-薄荷醇（trans-Piperitol）	40.209 4	0.450 9
	桉叶油醇（Eucalyptol）	18.110 7	0.211 5