虎乳芝提取物的护肤功效及安全性评价研究

doi:10.3969/j.issn.2097-2806.2026.06.008

摘要/Abstract

摘要：

以水提取的虎乳芝提取物为研究对象，通过抗氧化、抗糖化（损伤）、细胞增殖、相关基因表达测试，对其抗衰、修护功效进行了研究；通过一氧化氮抑制试验检测其抗炎效果；通过检测皮脂分泌基因表达、胞内脂质和脂滴，对其控油潜力进行评估，并通过皮肤刺激性试验对其安全性进行评价。结果发现，虎乳芝提取物能有效清除DPPH自由基，显著减少糖化产物的产生和损伤，并促进抗衰相关基因的表达（P<0.05），具有抗氧化、抗糖、紧致抗皱功效；能促进角质细胞的增殖，提升透明质酸合酶基因表达，具有修护功效；能显著抑制脂多糖诱导的一氧化氮的产生（P<0.05），具有舒缓功效；能显著抑制皮脂分泌基因表达，限制亚油酸诱导的皮脂合成和脂滴形成（P<0.05），具有潜在的控油能力。人皮肤模型刺激试验结果进一步证实了虎乳芝提取物具有较高的皮肤安全性。

关键词: 虎乳芝提取物, 抗衰, 抗糖化, 修护, 舒缓, 控油, 安全性评估

Abstract:

This study focused on the aqueous extract of Lignosus rhinocerus, investigating its anti-aging, repair, anti-inflammatory, and oil-control effects through antioxidant, anti-glycation, cell proliferation, and related gene expression tests. The anti-inflammatory effect was evaluated by the nitric oxide （NO） inhibition assay, and the oil-control potential was assessed by quantifying the expression of genes related to sebum secretion, as well as intracellular lipids, and lipid droplets. Skin safety was evaluated through skin irritation tests. The results show that the Lignosus rhinocerus extract effectively scavenges DPPH free radicals, significantly reduces the production of glycation products, and promotes the expression of anti-aging-related genes （P<0.05）, demonstrating antioxidant, anti-glycation, and anti-wrinkle effects. Furthermore, the extract also promotes keratinocyte proliferation and increases hyaluronic acid synthase gene expression, indicating repair effects. Moreover, it significantly inhibits the production of nitric oxide induced by lipopolysaccharide （P<0.05）, showing anti-inflammatory effects. It significantly suppresses the expression of genes related to sebum secretion, and limits linoleic acid-induced sebum synthesis and lipid droplet formation （P<0.05）, indicating potential oil-control ability. The results of the human skin model irritation test further confirm the high skin safety of the extract. This study demonstrates that the Lignosus rhinocerus extract has anti-aging, repair, anti-inflammatory, and oil-control activities, combining efficacy with safety, which makes it a highly valuable candidate for cosmetic ingredient development.

Key words: Lignosus rhinocerus extract, anti-aging, anti-glycation, repair, soothing, oil-control, safety assessment

中图分类号:

TQ658

陈漪汶, 郑雅莉, 李鑫, 黄健聪, 邝荣康, 谢小保. 虎乳芝提取物的护肤功效及安全性评价研究[J]. 日用化学工业（中英文）, 2026, 56(6): 764-775.

Yiwen Chen, Yali Zheng, Xin Li, Jiancong Huang, Rongkang Kuang, Xiaobao Xie. Study on the skincare effects and safety evaluation of the Lignosus rhinocerus extract[J]. China Surfactant Detergent & Cosmetics, 2026, 56(6): 764-775.

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参考文献 31

[1]	Fung S Y, Tan C S. Tiger milk mushroom (The Lignosus trinity) in Malaysia: a medicinal treasure trove[M]. Singapore: Springer Singapore, 2019: 349-369.
[2]	Kittimongkolsuk P, Roxo M, Li H, et al. Extracts of the tiger milk mushroom (Lignosus rhinocerus) enhance stress resistance and extend lifespan in Caenorhabditis elegans via the DAF-16/FoxO signaling pathway[J]. Pharmaceuticals, 2021, 14 (2) : 93.
[3]	Gu Y, Han J, Jiang C, et al. Biomarkers, oxidative stress and autophagy in skin aging[J]. Ageing Research Reviews, 2020, 59: 101036. doi: 10.1016/j.arr.2020.101036
[4]	Yamagishi S I. Advanced glycation end-products[J]. Dermato Endocrinology, 2013: 36-38.
[5]	符炽冰, 曹舒清, 张钰靖, 等. 皮肤光老化与自噬相关性的研究进展[J]. 黑龙江科学, 2022, 13 (16) : 71-73.
[6]	Zhou Y Y, Li Y, Jiang W Q, et al. MAPK/JNK signaling: A potential autophagy regulation pathway[J]. Bioscience Reports, 2015, 35 (3) : e00199.
[7]	胡露, 魏瑞敬, 聂艳峰, 等. 毛豆提取物的体外抗衰老活性研究[J]. 广东化工, 2021, 48 (7) : 28-30.
[8]	戴丽云, 魏玉洁, 陆震, 等. 糙米发酵滤液—— 一种具有抗衰老潜力的物质[J]. 日用化学工业, 2023, 53 (3) : 292-299.
[9]	Hyeon M J, In H C. Anti-inflammatory activity of Akebia quinata D. extracts by inhibiting MAPK and NF-κB signaling pathways in LPS-induced RAW 264.7 cells according to extraction solvents[J]. Basic research, 2025, 21: 315-323.
[10]	胡婷婷, 余茜, 莫小辉, 等. 尼古丁对SZ95人皮脂腺细胞增殖、凋亡及脂质合成的影响[J]. 中国皮肤性病学杂志, 2014, 28 (2) : 111-118.
[11]	Organisation for Economic Co-operation and Development (OECD). Test No. 439: in vitro skin irritation: reconstructed human epidermis test method[R]. Paris: OECD Publishing, 2010.
[12]	Fung S Y, Yap H Y Y, Ng S T, et al. In vitro inhibition of melanin formation and enhancement of collagen production by a mushroom sclerotial water extract from the tiger milk mushroom, Lignosus rhinocerus (Agaricomycetes), with no skin and eye irritation[J]. International Journal of Medicinal Mushrooms, 2022, 24 (5) : 19-32.
[13]	李铮, 孟庆宝. 抗氧剂264、2246及SD在天然胶乳中抗氧化基础因素的比较研究[C] // 中国化工学会橡胶专业委员会, 中国橡胶工业协会, 中国合成橡胶工业协会. 2004年国际橡胶会议论文集. 2004: 85-88.
[14]	郑子锋. 龙眼核多酚抗皮肤糖基化衰老作用的研究[D]. 无锡: 江南大学, 2021.
[15]	Yap H Y Y, Tan N H, Ng S T, et al. Inhibition of protein glycation by tiger milk mushroom [Lignosus rhinocerus (Cooke) Ryvarden] and search for potential anti-diabetic activity-related metabolic pathways by genomic and transcriptomic data mining[J]. Front Pharmacol, 2018, 9: 103. doi: 10.3389/fphar.2018.00103
[16]	肖益东, 蔡文斐, 郑赵敏, 等. 超声作用下虎乳灵芝多糖-硒纳米粒子的非酶糖基化抑制作用[J]. 食品科学, 2020 (15) : 41.
[17]	Adeosun C B, Olaseinde S, Opeifa A O, et al. Essential oil from the stem bark of Cordia sebestena scavenges free radicals[J]. Journal of Acute Medicine, 2013, 3 (4) : 138-141. doi: 10.1016/j.jacme.2013.07.002
[18]	Rodríguez-Cotto, Rosa I, Ortiz-Martínez, et al. Particle pollution in Rio de Janeiro, Brazil: Increase and decrease of pro-inflammatory cytokines IL-6 and IL-8 in human lung cells[J]. Environmental Pollution, 2014, 194 (9) : 112-120. doi: 10.1016/j.envpol.2014.07.010
[19]	方忆凡, 方嘉璇, 宋子欣, 等. 灰树花发酵液对UVB诱导人永生化角质形成细胞的修复作用[J]. 食品与发酵工业, 2015, 51 (18) : 295-303.
[20]	徐一凤, 王淳, 张梓菁, 等. 成纤维细胞老化分子机制与皮肤衰老的关系及研究进展[J] .中国美容医学, 2023, 32 (7) : 199-202.
[21]	Rajawat Y S, Bossis I. Autophagy in aging and in neurodegenerative disorders[J]. Hormones, 2008, 7 (1) : 46-61. doi: 10.14310/horm.2002.1111037
[22]	李坤杰, 黄豪, 郭燕妮. 透明质酸对敏感性皮肤屏障功能修复的研究进展[J]. 皮肤科学通报, 2017, 34 (4) : 403-407.
[23]	曲文杰. 透明质酸在皮肤护理上的应用[J]. 中国化妆品, 2020(8) : 94-97.
[24]	Voigt J, Driver V R. Hyaluronic acid derivatives and their healing effect on burns, epithelial surgical wounds, and chronic wounds: a systematic review and meta-analysis of randomized controlled trials[J]. Wound Repair and Regeneration, 2012, 20 (3) : 317-331. doi: 10.1111/j.1524-475X.2012.00777.x pmid: 22564227
[25]	Woodley D T, 魏骏节. 表皮真皮连接的重要性及进展[J]. 国外医学·皮肤病学分册, 1988 (4) : 224-227.
[26]	郑梅梅, 郑俭彬, 江自鲜, 等. 两种斯赤列提取物促进成骨细胞的增殖与分化[J]. 中国组织工程研究, 2023, 27 (11) : 1677-1682.
[27]	Lau B F, Abdullah N, Aminudin N, et al. Chemical composition and cellular toxicity of ethnobotanical-based hot and cold aqueous preparations of the tiger′s milk mushroom (Lignosus rhinocerotis)[J]. Journal of Ethnopharmacology, 2013, 150 (1) : 252-262. doi: 10.1016/j.jep.2013.08.034
[28]	Ellefsen C F, Lindstad L, Klau L J, et al. Investigation of the structural and immunomodulatory properties of alkali-soluble β-glucans from Pleurotus eryngii fruiting bodies[J]. Carbohydrate Polymers, 2023, 322: 121367. doi: 10.1016/j.carbpol.2023.121367
[29]	丁薇, 邹大进. 5α-还原酶对雄激素诱发痤疮的研究进展[J]. 中国皮肤性病学杂志, 2009, 23 (8) : 523-524.
[30]	Hyejin Kim, Jonghye, et al. Skin corrosion and irritation test of nanoparticles using reconstructed three-dimensional human skin model, EpiDerm^TM[J]. Toxicological Research, 2016, 32 (4) : 311-316. pmid: 27818733
[31]	Lee S S, Enchang F K, Tan N H, et al. Preclinical toxicological evaluations of the sclerotium of Lignosus rhinocerus (Cooke), the tiger milk mushroom[J]. Journal of Ethnopharmacology, 2013, 147(1) : 157-163. doi: 10.1016/j.jep.2013.02.027

Primer	Forward	Reverse
COL1	TGGTGGTTATGACTTTGGTTACGAT	TGTGCGAGCTGGGTTCTTTCTA
COL3A1	GGAGCTGGCTACTTCTCGC	GGGAACATCCTCCTTCAACAG
LAMA3	AACCAAAAAGTGCTCGGAAGAC	TCAGTAGGTGGTAAGCCCAAATC
LAMC2	TACAGAGCTGGAAGGCAGGATG	GTTCTCTTGGCTCCTCACCTTG
COL4A2	GTACTGCAACCCTGGTGATGTCTGC	GAATATCCGATCCACAAACTCCGCC
LC3A	CGGACATCTACGAGCAGGA	AGAGGCAGCTCAGTTCAGGA
ATG5	AACTGAAAGGGAAGCAGAACCA	CCATTTCAGTGGTGTGCCTTC
BECLIN1	AGCTGCCGTTATACTGTTCTG	ACTGCCTCCTGTGTCTTCAATCTT
HAS2	TTCTTTATGTGACTCATCTGTCTCACCGG	ATTGTTGGCTACCAGTTTATCCAAACG

成分	含量/ （mg/g）
碳水化合物	817.6±1.5
蛋白质	115.0±0.1
总酚	2.9±0.1

No.	保留时间/min	分子式	分子量	面积/%	名称
1	5.628	C₂H₆O	46.04	2.76	Dimethyl ether
2	7.304	C₅H₁₂O₂	104.08	0.09	（2R, 4R） - （-） -Pentanediol
3	21.455	C₁₉H₃₀O₃	306.22	4.21	Pentanoic acid, 5-hydroxy-, 2, 4-di-t-butylphenyl esters
4	24.155	C₁₂H₈O₅	232.04	2.35	1, 4-Naphthoquinone, 6-acetyl-2, 5-dihydroxy-
5	30.231	C₁₃H₂₈	184.22	1.07	Decane, 3, 3, 8-trimethyl-
6	30.304	C₁₇H₂₄O₃	276.17	10.15	7, 9-Di-tert-butyl-1-oxaspiro （4, 5） deca-6, 9-diene-2, 8-dione
7	31.176	C₁₇H₂₄O₄	292.17	1.66	Phthalic acid, 2-ethylbutyl propyl ester
8	31.642	C₁₈H₃₆O₂	284.27	18.39	Hexadecanoic acid, ethyl ester
9	34.023	C₄H₇NO	85.05	0.12	Propane, 1-isocyanato-
10	34.802	C₂₀H₃₈O₂	310.29	10.01	（E） -9-Octadecenoic acid ethyl ester
11	35.255	C₂₀H₄₀O₂	312.3	6.80	Octadecanoic acid, ethyl ester
12	39.141	C₂₃H₃₂O₂	340.24	10.60	Phenol, 2, 2'-methylenebis[6- （1, 1-dimethylethyl） -4-methyl-
13	41.085	C₇H₁₂O₂	128.08	2.43	2, 4-Heptanedione
14	44.289	C₇H₇Cl₂NO	190.99	4.75	Clopidol
15	48.392	C₁₁H₇N₅	209.07	0.34	1, 3, 6, 9b-Tetraazaphenalene-4-carbonitrile, 2-methyl-
16	51.535	C₁₀H₁₁NO₃	193.07	2.07	Terephthalaldehydic acid, methyl ester, p- （O-methyloxime）
17	52.248	C₂₃H₂₃NO₂	345.17	8.35	Benzamide, 2-methoxy-N-benzyl-N-phenethyl-
18	52.867	C₁₈H₂₆O₄	306.18	0.83	Phthalic acid, ethyl 4-octyl ester
19	53.189	C₂₄H₂₂	310.17	2.28	1, 2, 3-Triphenyl-3-isopropyl-cyclopropene
20	53.35	C₁₀H₁₁NO₃	193.07	4.17	2, 4, 6-Trimethoxybenzonitrile
21	56.463	C₁₇H₁₄O₄	282.09	2.49	4H-1-Benzopyran-4-one, 6, 7-dimethoxy-3-phenyl-
22	56.794	C₂₀H₂₂O₅	342.15	1.35	Terephthalic acid, isobutyl 2-phenoxyethyl ester
23	57.778	C₁₄H₂₇N	209.21	0.86	（5R, 8R, 8aS） -5, 8-Dipropyloctahydroindolizine
24	57.867	C₂₀H₂₃NO₄	341.16	1.86	2- （3, 5-Dimethoxyphenyl） -5, 6-dimethoxy-1, 3-dimethyl-1H-indole-