高效液相色谱法测定防晒类化妆品中邻甲氨基苯甲酸甲酯

doi:10.3969/j.issn.1001-1803.2022.12.013

摘要/Abstract

摘要：

建立了一种高效液相色谱法测定防晒类化妆品中邻甲氨基苯甲酸甲酯含量的方法。样品经提取、超声、过滤后，采用Welch Xtimate^®C₁₈色谱柱分离，高效液相色谱-二极管阵列检测器进行定性、定量分析。在本实验条件下，邻甲氨基苯甲酸甲酯能与防晒类化妆品中常见的22种防晒剂进行分离，其质量浓度与峰面积之间呈良好的线性关系（r=0.999 6），检出限为0.009%，定量限为0.029%，在高、中、低三种质量浓度的添加回收率实验中，回收率在99.33%~111.5%之间，RSD在0.3%~3.0%之间。该方法专属性强、准确度高，适用于防晒类化妆中邻甲氨基苯甲酸甲酯含量的测定。

关键词: 邻甲氨基苯甲酸甲酯, 防晒类化妆品, 高效液相色谱法

Abstract:

A high performance liquid chromatography（HPLC） method was established for the determination of methyl methylanthranilate in sunscreen cosmetics. After extraction, sonication and filtration, the samples were separated by Welch Xtimate^® C₁₈ chromatographic column, and qualitative and quantitative analysis were carried out by HPLC with PDA detector. Under the experimental conditions, there is a good linear relationship between peak areas and methyl methylanthranilate concentrations with the r value is 0.999 6. The detection limit is 0.009%, and the limit of quantification is 0.029%. The average recoveries are in the range of 99.33%-111.5% and the relative standard deviations are in the range of 0.3%-3.0%. This accurate and exclusive method is suitable for the qualitative and quantitative analysis of methyl methylanthranilate in sunscreen cosmetics.

Key words: methyl methylanthranilate, sunscreen cosmetics, high performance liquid chromatography

中图分类号:

TQ658

吴志珊,姜成君,殷帅,黄海萍,章为. 高效液相色谱法测定防晒类化妆品中邻甲氨基苯甲酸甲酯[J]. 日用化学工业（中英文）, 2022, 52(12): 1348-1352.

Wu Zhishan,Jiang Chengjun,Yin Shuai,Huang Haiping,Zhang Wei. Determination of methyl methylanthranilate by high performance liquid chromatography in sunscreen cosmetics[J]. China Surfactant Detergent & Cosmetics, 2022, 52(12): 1348-1352.

图/表 9

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参考文献 9

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时间/min	φ（A）/%	φ（B）/%	φ（C）/%
0	18	2	80
20	25	45	30
30	25	45	30
35	20	40	40
38	50	45	5
39.5	50	45	5
40	30	20	50
41	65	30	5
49	30	65	5
50	18	2	80
55	18	2	80

质量浓度/（μg/mL）	回收率-1/%	回收率-2/%	回收率-3/%	回收率-4/%	回收率-5/%	回收率-6/%	平均值/%	RSD/%
1.198 4	103.3	105.8	101.3	106.0	102.9	105.2	104.1	1.9
119.84	105.7	106.3	106.0	106.3	106.5	106.4	106.2	0.3

质量浓度/ （μg/mL）	Day-1/%	Day-2/%	Day-3/%	平均值/%	RSD/%
1.198 4	103.2	104.9	102.9	103.7	1.1
119.84	107.4	104.2	106.8	106.1	1.7

基质	回收率/%			RSD/%
基质	1.198 4 μg/mL	11.984 μg/mL	119.84 μg/mL	1.198 4 μg/mL	11.984 μg/mL	119.84 μg/mL
水剂类基质	100.6	103.4	105.7	1.6	0.3	0.8
霜剂类基质	103.6	106.1	105.2	2.5	0.8	1.9
乳液类基质	111.5	107.1	99.33	3.0	1.5	2.9

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