复方精油对小鼠B16细胞内黑色素生成影响

doi:10.3969/j.issn.1001-1803.2021.03.008

摘要/Abstract

摘要：

为探讨一款复方精油抑制酪氨酸酶活性和黑色素含量的效果,采用气相色谱与质谱联用技术（GC-MS）对一款复方精油进行成分分析;以促黑素细胞激素（α-MSH）诱导黑色素高表达的小鼠黑色素瘤细胞（B16细胞）为模型,利用台盼蓝染色记数细胞、CKK8法测定复方精油对黑色素细胞活力的影响;分别采用酶联免疫吸附法（ELISA）和多巴氧化法测定细胞内的黑色素含量以及酪氨酸酶活性。结果显示,该复方植物精油中含有蒎烯（26.19%）、芳樟醇（12.98%）、檀香醇（9.39%）、乙酸芳樟酯（9.16%）等化合物;当复方精油质量浓度小于0.1 g/L时对B16细胞没有毒性,细胞增殖率高于90%;不同质量浓度的复方精油对α-MSH诱导的B16细胞内的黑色素含量和酪氨酸酶活性均有显著的抑制作用,当质量浓度为0.1 g/L时,对B16细胞内黑色素合成的抑制率为36.03%,对细胞内酪氨酸酶活性的抑制率为51.27%,其抑制黑色素生成效果最佳。综上所述,该复方植物精油能够显著抑制黑色素合成,抑制B16细胞内酪氨酸酶活性。

关键词: 复方精油, 黑色素含量, 酪氨酸酶抑制, B16黑色素瘤细胞

Abstract:

In order to investigate the inhibitory effect of a blend essential oil on tyrosinase activity and melanin content, the composition of this blend essential oil was analyzed by GC-MS. Taking mouse melanoma cells （B16） with high melanin expression induced by melanocyte-stimulating hormone （α-MSH） as the model, the effects of the blend essential oil on melanocyte vitality were determined by trypan blue staining and CKK8 method. Enzyme-linked immunosorbent assay （ELISA） and dopa oxidation method were used to measure melanin content and tyrosinase activity in cells. The results showed that the blend essential oil contained a large number of compounds such as pinene （26.19%） , linalool （12.98%） , santalol （9.39%） , linalyl acetate （9.16%） , etc.; the blend essential oil had no toxicity to B16 cells when the mass concentration was less than 0.1 g/L, and the cell proliferation rate was higher than 90%; the blend essential oil at different mass concentrations could significantly inhibit the tyrosinase activity and melanin content in B16 cells induced by α-MSH. When the mass concentration of blend essential oil was 0.1 g/L,it had the best inhibitory effect on melanin production, and the inhibitory rates on tyrosinase activity and melanin synthesis were 51.27% and 36.03%, respectively. In conclusion, the blend plant essential oil could significantly inhibit melanin synthesis and inhibit the tyrosinase activity in B16 cells.

Key words: compound essential oil, melanin content, tyrosinase inhibition, B16 melanoma cell

中图分类号:

TQ658

王莹,唐寅,吴亚妮. 复方精油对小鼠B16细胞内黑色素生成影响[J]. 日用化学工业, 2021, 51(3): 214-219.

WANG Ying,TANG Yin,WU Ya-ni. Effects of blend essential oil on melanogenesis in B16 cells of mice[J]. China Surfactant Detergent & Cosmetics, 2021, 51(3): 214-219.

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序号	化学成分	保留时间/min	相对含量/%
1	松油烯（Terpinene）	13.22	0.01
2	α-蒎烯（α-Pinene）	14.41	26.19
3	莰烯（Camphene）	14.81	1.31
4	月桂烯（Myrcene）	15.97	6.9
5	邻伞花烃（o-Cymene）	17.04	0.11
6	柠檬烯（Limonene）	17.53	8.81
7	桉叶醇（Eucalyptol）	17.64	1.63
8	马鞭草烯醇（Verbenol）	19.43	0.29
9	芳樟醇（Linalool）	19. 85	12.98
10	顺式马鞭草烯醇（cis-Verbenol）	21.32	0.98
11	松油烯-4-醇（Terpinen-4-ol）	22.69	4.36
12	萜品醇（Terpineol）	23.07	1.07
13	香茅醇（Citronellol）	23.86	1.01
14	乙酸芳樟酯（Linalyl acetate）	24.71	9.16
15	乙酸冰片酯（Bornyl acetate）	25.90	0.66
16	乙酸桃金娘烯酯（Myrtenyl acetate）	26.20	0.07
17	乙酸松油酯（Terpinyl acetate）	27.72	0.06
18	胡椒稀（Copaene）	28.63	0.14
19	β-倍半水芹烯（β-sesquiphellandrene）	29.12	3.19
20	榄香烯（Elemene）	29.47	0.02
21	石竹烯（Caryophyllene）	30.24	4.97
22	蛇麻烯（Humulene）	31.21	1.58
23	红没药醇（Bisabolol）	31.45	0.01
24	大根香叶烯（Germacrene D）	31.92	1.15
25	依兰油烯（Muurolene）	32.32	2.02
26	甜没药烯（Bisabolene）	32.45	0.15
27	橙花椒醇（Nerolidol）	33.79	0.15
28	石竹烯氧化物（Caryophyllene oxide）	34.73	0.72
29	桃金娘烯醇（Myrtenol）	36.69	0.84
30	檀香醇（Santalol）	38.34	9.39
31	水芹烯（Phellandrene）	42.36	0.04
32	金合欢烯（Farnesene）	43.61	0.02