欢迎访问《日用化学工业（中英文）》，今天是
日用化学工业（中英文）

日用化学工业 ›› 2021, Vol. 51 ›› Issue (11): 1045-1051.doi: 10.3969/j.issn.1001-1803.2021.11.002

• 基础研究 • 上一篇    下一篇

固载过氧化氢酶微球清除家庭漂白后残留过氧化氢的研究

陈佳琳,刘刚,孙梦梦,郭婷,孟涛()   

  1. 西南交通大学 生命科学与工程学院,四川 成都 610031
  • 收稿日期:2021-02-13 修回日期:2021-09-22 出版日期:2021-11-22 发布日期:2021-11-19
  • 通讯作者: 孟涛
  • 基金资助:
    国家自然科学基金资助项目(21776230);四川省科技厅重点研发项目(2020YFG0092)

Removal of residual hydrogen peroxide after bleaching by microspheres containing catalase

Chen Jialin,Liu Gang,Sun Mengmeng,Guo Ting,Meng Tao()   

  1. School of Life Sciences and Engineering, South West Jiaotong University, Chengdu, Sichuan 610031, China
  • Received:2021-02-13 Revised:2021-09-22 Online:2021-11-22 Published:2021-11-19
  • Contact: Tao Meng

RichHTML

10

PDF (PC)

252

赞

摘要：

利用仿生矿化法制备了固载过氧化氢酶的碳酸钙微球,研究了该载酶微球的包封情况、在苛刻环境中的稳定性和清除漂白纱布后残留过氧化氢的效果。结果表明,该载酶微球粒径约11 μm,孔径为5.53 nm,能有效封装住过氧化氢酶;在过氧化氢酶质量浓度为0.15 mg/mL的条件下,该微球对过氧化氢酶具有最高负载量为24.26 mg/g,此时酶包封率为98%,酶活力保留率为72%。该载酶微球与游离酶相比提升了热稳定性（80 ℃条件下仍能保留38.99%的相对酶活力）、改变了过氧化氢酶对不同pH环境耐受能力的趋势（最适酶活力pH值由pH 8偏移至pH 11）,提升了耐表面活性剂性质（在十二烷基硫酸钠环境中仍保留了85.82%的相对酶活力）。且该载酶微球能在25 min完全清除漂白纱布后残留的过氧化氢。

关键词: 过氧化氢, 过氧化氢酶, 碳酸钙, 漂白

Abstract:

Calcium carbonate microspheres containing catalase were prepared by the method of bionic mineralization. The size and pore size of the enzyme-carrying microspheres were measured; the encapsulation efficiency, loading capacity and stability of the enzyme-carrying microspheres in harsh environments were studied; the removal of residual hydrogen peroxide from the gauze after bleaching was studied. The results show that the enzyme-carrying microspheres have a particle size of approximately 11 μm. From the nitrogen adsorption-desorption isotherm and pore size distribution diagram, it can be seen that CAT@CaCO3 microspheres have uniformly distributed pores （approximately 5.53 nm）, which can well encapsulate catalase （approximately diameter 6 nm×10 nm）, allowing the substrate H2O2 and the product H2O to diffuse freely. Under the condition that the mass concentration of catalase is 0.15 mg/mL, the microsphere has a maximum load of 24.26 mg/g for catalase. Meanwhile, the enzyme encapsulation rate is 98%, and the enzyme activity retention rate is 72%, indicative of the mildness of the method of enzyme immobilization. Compared with free enzymes, the enzyme-carrying microspheres have improved thermal stability （38.99% of relative enzyme activity can still be retained at 80 ℃） and have changed the trend of catalase tolerance to different pH environments （the optimal pH for the enzyme activity shifts from pH 8 to pH 11）. This phenomenon may be ascribedto the mineralization and fixation of calcium carbonate changing the conformation of the enzyme. To a certain extent, the conformation of CAT is changed, resulting in a different tendency of pH tolerance from free enzymes. In addition, the anti-surfactant performance of the enzyme-carrying microspheres is improved （85.82% of relative enzyme activity remains in the presence of sodium lauryl sulfate）. The reasons might be that the calcium carbonate mineral shell can stabilize the three-dimensional structure of the enzyme during the process of immobilizing the enzyme, resulting in the enhanced stability of the immobilized enzyme. Finally, the enzyme-carrying microspheres can completely remove the residual hydrogen peroxide from the gauze after bleaching within 25 min, which has a faster removal rate than the free enzyme （35 min）.

Key words: hydrogen peroxide, catalase, calcium carbonate, bleach

中图分类号: 

  • TQ423