神经酰胺纳米乳液的制备及透皮性能研究

doi:10.3969/j.issn.1001-1803.2021.10.004

摘要/Abstract

摘要：

为了提高神经酰胺的应用稳定性和生物利用率,采用高压均质法制备了神经酰胺（Ceramide III,Cer3）纳米乳液,研究了卵磷脂用量、甘油与水质量比、均质压力、循环次数及植物鞘氨醇添加量对乳液粒径及稳定性的影响,发现当卵磷脂质量分数为4%,甘油与水质量比为7:1,均质压力为90 MPa,循环9次,植物鞘氨醇质量分数为1.5%时,载体在各温度下具有良好的储藏稳定性;此时载体呈凝胶状并携带正电,Zeta电位为36.67 mV,粒径为200 nm左右,添加植物鞘氨醇显著提高了载体的高温稳定性,奥氏熟化速率由6.06降至1.53,并且由于植物鞘氨醇的添加,Cer3透皮含量由3.02 μg/cm ²增至7.12 μg/cm ²,透皮性能得到显著提高。

关键词: 神经酰胺, 储藏稳定性, 植物鞘氨醇, 奥氏熟化, 透皮性能

Abstract:

Ceramide is an important lipid in the stratum corneum of skin and plays an indispensable role in maintaining the normal physiological functions of skin. The skin contains the most abundant types of ceramides and there are currently 15 types of ceramides that have been detected. And among them, ceramide3 （Cer3） has the highest content in the skin and is an essential ingredient for regulating skin barrier function. However, Cer3 is very easy to crystallize when directly applied and thus it needs a carrier to improve its stability during application. Therefore, to improve the stability and bioavailability of Cer3, Cer3 nanoemulsions were prepared by high-pressure homogenization method. The effects of the amount of lecithin, the mass ratio of glycerol to water, the homogenization pressure, the number of cycles and the amount of phytosphingosine on the particle size and stability of the emulsions were studied. It is found that when the mass fraction of lecithin is 4%, the mass ratio of glycerol to water is 7:1, the average pressure is 90 MPa and the number of cycles is 9, the emulsion has good stability. Furthermore, phytosphingosine was added to the system to improve the storage stability of Cer3 nanoemulsion at high temperature. The results show that when the mass fraction of phytosphingosine is 1.5%, the Ostwald ripening rate of Cer3 nanoemulsion is decreased from 6.06 to 1.53 and the Cer3 nanoemulsion is gel-like at this time. It is found that the nanoemulsion particles are spherical and have a particle size of approximately 200 nm as observed by TEM. The results of Zeta potential show that the Cer3 nanoemulsion carries a positive charge and the potential is 36.67 mV. The transdermal performance of the Cer3 nanoemulsions with different mass fractions of phytosphingosine was also studied. It is found that the transdermal content of Cer3 is closely related to the potential value of the Cer3 nanoemulsion. When the mass fraction of phytosphingosine is 1.5%, the transdermal content of Cer3 is increased from 3.02 μg/cm ² to 7.12 μg/cm ², indicating that the addition of phytosphingosine can effectively improve the transdermal performance of Cer3.

Key words: ceramide, storage stability, phytosphingosine, Ostwald ripening, transdermal performance

中图分类号:

TQ658

王玙璇,梁蓉,骆晔媛. 神经酰胺纳米乳液的制备及透皮性能研究[J]. 日用化学工业, 2021, 51(10): 949-955.

Wang Yuxuan,Liang Rong,Luo Yeyuan. Study on the preparation and transdermal performance of the ceramide nanoemulsion[J]. China Surfactant Detergent & Cosmetics, 2021, 51(10): 949-955.

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