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日用化学工业（中英文）

日用化学工业 ›› 2017, Vol. 47 ›› Issue (4): 232-240.doi: 10.13218/j.cnki.csdc.2017.04.011

• 分析与检测 • 上一篇    

高效液相色谱法测定糖苷类表面活性剂产品中糖类物质的含量

张军, 杨秀全, 周媛, 孙永强, 李运玲   

  1. 中国日用化学工业研究院,山西 太原 030001
  • 收稿日期:2016-11-17 发布日期:2019-03-20
  • 通讯作者: 杨秀全,教授级高工,硕士,电话:(0351)4079081,E-mail:apggroup@sina.com。
  • 作者简介:张 军(1983-),男,山西人,工程师,硕士,电话:(0351)4063540,E-mail:zfj0915@163.com。
  • 基金资助:
    “十二五”国家科技支撑计划资助项目(2014BAE03B03);山西省青年科技研究基金资助项目(2014021015-6)

Determination of monose and polysaccharides in glucoside-based surfactant products by HPLC

ZHANG Jun, YANG Xiu-quan, ZHOU Yuan, SUN Yong-qiang, LI Yun-ling   

  1. China Research Institute of Daily Chemical Industry,Taiyuan,Shanxi 030001,China
  • Received:2016-11-17 Published:2019-03-20

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摘要： 建立了同时测定糖苷类表面活性剂产品中单糖和多糖含量的高效液相色谱法。采用Zorbax Eclipse Plus C18色谱柱(4.6 mm×100 mm,3.5 μm)分离,甲醇-水梯度洗脱,流速1.0 mL/min,蒸发光散射检测器检测。用葡萄糖和麦芽三糖为对照品,外标两点法同时测定糖苷产品中单糖和多糖的含量。结果表明,单糖和多糖的检出限分别约为1.5和2.0 μg/L,回收率分别为100.4%和99.1%。与既有方法相比,此方法可以同时分析产品中残留原料糖和副产物多糖的含量,且在分析非还原性单糖方面具有独特优势。

关键词: 糖基表面活性剂, 单糖, 多糖, 高效液相色谱法

Abstract: A HPLC method for simultaneous determination of contents of monose and polysaccharides in glucoside-based surfactant products was established.The method adopts evaporative light-scattering detector and Zorbax Eclipse Plus C18 column (4.6 mm×100 mm,3.5 μm) with methanol and water as the mobile phase at a rate of 1.0 mL/min.The contents of saccharides were calculated by external standard two-point method using glucose and maltotriose as control samples.Results show that the detection limit of monose and polysaccharides is 1.5 and 2.0 μg/L respectively while the recovery is 100.4% and 99.1% respectively.As comparing with the conventional methods,this method displays unique superiority in determination of non-reducing monose and the residual glucose from raw material for preparation of glucoside-based surfactants as well as the by-products polysaccharides can be determined simultaneously.

Key words: glucoside-based surfactant, monose, polysaccharides, HPLC

中图分类号: 

  • TQ423.2