用于组合活性物透皮共递送的环糊精复合物传质体的制备与表征

doi:10.3969/j.issn.1001-1803.2020.08.004

Abstract

Abstract:

Active-in-cyclodextrin-in-transfersomes （DCTs）, which encapsulated phenylethyl resorcinol （PR） and resveratrol/hydroxypropyl-β-cyclodextrin （RSV/HP-β-CD） inclusion complexes in the phospholipid bilayer and aqueous compartments of the transfersomes, were prepared by thin film hydration using lecithin, cholesterol, and surfactant as encapsulation materials. The effects of edge activator, their concentrations and addition methods on the particle size, PDI, Zeta potential and encapsulation efficiency of DCTs were studied. Results show that the particle size of DCTs prepared by 0.012 mol/L Tween 20 is 112.43 nm, PDI is 0.16, Zeta potential is -34.90 mV, and the high entrapment efficiency of PR and RSV are achieved as 77.07% and 60.67%, respectively. Through in vitro release, transdermal, cytotoxicity and melanin content in B16-F10 cells of DCTs, it was found that DCTs achieved drug sustained release and prevented drug degradation, reduced the permeability difference of transdermal delivery of combination drugs, decreased the cytotoxicity of free drugs, enhanced the synergistic effect of the combination drug, and reduced the melanin content of B16-F10 cells.

Key words: whitening, cyclodextrin, transfersomes, phenylethyl resorcinol, resveratrol, transdermal, co-delivery

CLC Number:

TQ658

SHE Lan-lan,ZHAO Bing-tian,YANG Cheng. Preparation and characterization of active-in-cyclodextrin-in-transfersomes for combined drug transdermal co-delivery[J].China Surfactant Detergent & Cosmetics, 2020, 50(8): 529-535.

Figures/Tables 8

Tab. 1

Tab. 2

Fig. 1

Fig. 2

Fig.3

Fig.4

Fig.5

Fig.6

References 17

[1]	Wang Y, Hao M M, Sun Y, et al. Synergistic promotion on tyrosinase inhibition by antioxidants[J]. Molecules, 2018,23(1) : 106. doi: 10.3390/molecules23010106
[2]	Fan H, Li Y, Huang Y, et al. Preparation and evaluation of phenylethyl resorcinol liposome[J]. Integrated Ferroelectrics, 2014,151(1) : 89-98. doi: 10.1080/10584587.2014.899873
[3]	Rodríguez-Cabo T, Rodríguez I, Ramil M, et al. Comprehensive evaluation of the photo-transformation routes of trans-resveratrol[J]. Journal of Chromatography A, 2015,1410:129-139. doi: 10.1016/j.chroma.2015.07.088 pmid: 26253832
[4]	Al-Lazikani B, Banerji U, Workman P. Combinatorial drug therapy for cancer in the post-genomic era[J]. Nature Biotechnology, 2012,30(7) : 679. doi: 10.1038/nbt.2284
[5]	Wang A Z, Langer R, Farokhzad O C. Nanoparticle delivery of cancer drugs[J]. Annual Review of Medicine, 2012,63:185-198. doi: 10.1146/annurev-med-040210-162544
[6]	Gadde S. Multi-drug delivery nanocarriers for combination therapy[J]. MedChemComm, 2015,6(11) : 1916-1929. doi: 10.1039/C5MD00365B
[7]	You J O, Guo P, Auguste D T. A drug‐delivery vehicle combining the targeting and thermal ablation of HER2+ breast‐cancer cells with triggered drug release[J]. Angewandte Chemie International Edition, 2013,52(15) : 4141-4146. doi: 10.1002/anie.201209804 pmid: 23494862
[8]	Gidwani B, Vyas A. A comprehensive review on cyclodextrin-based carriers for delivery of chemotherapeutic cytotoxic anticancer drugs[J]. BioMed Research International, 2015,2015(10) : 1-15.
[9]	Anwekar H, Patel S, Singhai A K. Liposome-as drug carriers[J]. International Journal of Pharmacy & Life Sciences, 2011,2(7) : 945-951.
[10]	Amnuaikit T, Limsuwan T, Khongkow P, et al. Vesicular carriers containing phenylethyl resorcinol for topical delivery system; liposomes, transfersomes and invasomes[J]. Asian Journal of Pharmaceutical Sciences, 2018,13(5) : 472-484. doi: 10.1016/j.ajps.2018.02.004 pmid: 32104421
[11]	McCormack B, Gregoriadis G. Drugs-in-cyclodextrins-in liposomes: a novel concept in drug delivery[J]. International Journal of Pharmaceutics, 1994,112(3) : 249-258. doi: 10.1016/0378-5173(94)90361-1
[12]	Cadena P G, Pereira M A, Cordeiro R B S, et al. Nanoencapsulation of quercetin and resveratrol into elastic liposomes[J]. Biochimica et Biophysica Acta (BBA) -Biomembranes, 2013,1828(2) : 309-316. doi: 10.1016/j.bbamem.2012.10.022
[13]	El Zaafarany G M, Awad G A S, Holayel S M, et al. Role of edge activators and surface charge in developing ultradeformable vesicles with enhanced skin delivery[J]. International Journal of Pharmaceutics, 2010,397(1-2) : 164-172. doi: 10.1016/j.ijpharm.2010.06.034 pmid: 20599487
[14]	Wang Jianguo, Jiang Lili, Hui Fei, et al. Study on the interaction of sodium deoxycholate with supported bilayer lipid membrane by electrochemistry method[J]. Acta Chimica Sinica, 2007,65(3) : 239-245.
[15]	Liang Wei. Study on the bufalin liposome[D]. Shanghai: Shanghai Institution of Pharmaceutical Industry, 2000.
[16]	Peralta M F, Guzmán M L, Pérez A P, et al. Liposomes can both enhance or reduce drugs penetration through the skin[J]. Scientific Reports, 2018,8(1) : 13253. doi: 10.1038/s41598-018-31693-y pmid: 30185887
[17]	How C W, Rasedee A, Manickam S, et al. Tamoxifen-loaded nanostructured lipid carrier as a drug delivery system: characterization, stability assessment and cytotoxicity[J]. Colloids and Surfaces B: Biointerfaces, 2013,112:393-399. doi: 10.1016/j.colsurfb.2013.08.009 pmid: 24036474

编码	条件
	ρ（油相）/（g·L^-1）		c（活性物）/（mol·L^-1）		c（膜软化剂）/（mol·L^-1）
	SPC	CHOL	PR	RSV	SDC	T80	T20
S-1	3	0.6	0.007	0.007	0.006 （O）	-	-
S-2	3	0.6	0.007	0.007	0.012 （O）	-	-
T8-1	3	0.6	0.007	0.007	-	0.006 （O）	-
T8-2	3	0.6	0.007	0.007	-	0.012 （O）	-
T2-1	3	0.6	0.007	0.007	-	-	0.006 （O）
T2-2	3	0.6	0.007	0.007	-	-	0.012 （O）
T2-3	3	0.6	0.007	0.007	-	-	0.006 （A）

编码	表征
	粒径/nm	PDI	包封率/%		Zeta电位/mV
	粒径/nm	PDI	PR	RSV	Zeta电位/mV
S-1	145.43±3.35	0.10±0.01	66.88±0.41	44.25±0.90	-56.13±1.20
S-2	110.00±2.70	0.24±0.03	54.17±2.20	9.90±1.94	-25.78±4.52
T8-1	113.93±3.74	0.18±0.01	69.04±0.23	48.79±0.83	-39.32±4.12
T8-2	97.90±2.43	0.18±0.01	72.11±1.06	53.37±2.20	-30.55±3.55
T2-1	112.43±4.73	0.16±0.01	72.17±1.35	53.16±1.95	-34.90±5.94
T2-2	104.17±2.37	0.19±0.01	77.07±1.05	60.67±2.11	-32.74±5.12
T2-3	113.73±2.93	0.14±0.01	70.59±0.81	51.83±1.85	-31.85±3.70

Preparation and characterization of active-in-cyclodextrin-in-transfersomes for combined drug transdermal co-delivery

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 17

Related Articles 15

Metrics

Comments

Recommended 10

[1]	Yaoyao Li. Study on the anti-aging and antioxidant effects of isosinensetin [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 313-319.
[2]	Ning Li, Ennian Li, Hongbo Chen, Fang Cheng, Hengfang Zou, Hongpeng Chen. Advances of functional components in whitening cosmetic [J]. China Surfactant Detergent & Cosmetics, 2024, 54(1): 80-89.
[3]	Zhang Wanping, Gai Houchen, Zhang Dongmei, Jiang Wen, Zhu Guangyong. Research progress of cyclodextrin encapsulation technology and its application on cosmetic ingredients [J]. China Surfactant Detergent & Cosmetics, 2023, 53(7): 808-815.
[4]	Han Yudi, Jin Liying, Sun Xihan, Lin Changqing, Cui Chengbi. Preparation of a lotion containing Rhodiola rosea and Panax ginseng and its antioxidant and whitening effects [J]. China Surfactant Detergent & Cosmetics, 2023, 53(6): 665-672.
[5]	Yang Xiaoyu, Liu Jinjun, Liu Lei, He Congfen, Bi Yongxian, Li Hao. Progress in metabolic mechanism and research methods of melanin production [J]. China Surfactant Detergent & Cosmetics, 2023, 53(10): 1194-1203.
[6]	Yan Jun,Wang Rong,Li Zehua,Zhang Liyuan,Cheng Qiaoyuan,Yan Linqi. Simultaneous determination and usage analysis of six functional ingredients in whitening cosmetics [J]. China Surfactant Detergent & Cosmetics, 2022, 52(7): 791-796.
[7]	Wang Dongdong,Sun Qianru,Wang Ziwen,Fang Jiaxuan,Li Meng,Wang Changtao. Study on the anti-aging and whitening effects of grape seed fermentation broth [J]. China Surfactant Detergent & Cosmetics, 2022, 52(5): 545-552.
[8]	Li Ziyi,Liu Yan,Zhang Zhao,Wu Huiqin. Preparation of a papain-embedding biological membrane material and its application in the skin-whitening products [J]. China Surfactant Detergent & Cosmetics, 2022, 52(3): 294-301.
[9]	Zhang Hongyan,Shi Xiaofeng,Wang Xiaoqiao,Liu Xingguo,Liu Yapeng,Li Yun. Study on transdermal absorption and skin irritation of fluorescent whitening agents in facial mask [J]. China Surfactant Detergent & Cosmetics, 2022, 52(3): 322-327.
[10]	Jin Jiaying,Chen Lu,Wang Xinzhi,Liu Rui,Wu Hao. Whitening efficacy and mechanism study of compound of Ganoderma atrum （mushroom） extract and pearl extract [J]. China Surfactant Detergent & Cosmetics, 2022, 52(2): 166-171.
[11]	He Ruiyuan,Wang Shuo,Wei Guili,Gong Xiaomei,Wu Peiying,Miao Jianhua. Study of whitening effect and the mechanism of Zhuang medicine Poecilobdella manillensis extract [J]. China Surfactant Detergent & Cosmetics, 2022, 52(1): 35-43.
[12]	Xue Wanting,Li Li,Dong Yinmao,Guo Miaomiao. Status and new development tendency of cosmetics whiteningefficacy evaluation [J]. China Surfactant Detergent & Cosmetics, 2021, 51(9): 890-896.
[13]	Ren Qianqian,Wu Hua,Jin Jianming. Botanical cosmetic ingredient (IV) Research and development of skin whitening ingredients from plant extracts with melanogenesis signaling pathway inhibiting effect [J]. China Surfactant Detergent & Cosmetics, 2021, 51(7): 590-597.
[14]	Zhang Xingqi,He Jingyu,Gong Shengzhao,Liu Linfeng. Study on the differences in the composition and the whitening and anti-allergy activities of different kinds of licorice [J]. China Surfactant Detergent & Cosmetics, 2021, 51(7): 648-654.
[15]	FU Hao,WU Di,BING Xue,ZHANG Jia-chan,WANG Chang-tao,LI Meng. Study on the skin care effect of oat β-glucan extracted by bidirectional fermentation [J]. China Surfactant Detergent & Cosmetics, 2021, 51(4): 324-330.