利用3D皮肤模型研究不同分子量透明质酸的抗刺激作用

doi:10.13218/j.cnki.csdc.2016.06.008

Abstract

Abstract: Sodium dodecyl sulfate (SDS) mixed with hyaluronic acid (HA) of separate molecular weight were applied on HaCaT cells and 3D skin model separately.After action of 24 h,the cell viability was detected by MTT assay and the level of IL-1α by ELISA method.The difference of exogenous effect of HA to reduce irritation induced by SDS on cell viability and IL-1α releasing between the two in vitro test models was studied.In conclusion,3D skin model can be used to test the anti-irritation effect of HA in vitro.Meanwhile,the exposure and testing results of 3D skin model are more close to human situation and can provide further mechanism information as comparing with the monolayer cell.

Key words: sodium dodecyl sulfate, 3D skin model, keratinocyte, hyaluronic acid, irritation, cytokines

CLC Number:

TQ423.11

YU Huan,CHEN Yu,CHENG Shu-jun,QIN Yao. Study of anti-irritation effect of hyaluronic acid using 3D skin model[J].China Surfactant Detergent & Cosmetics, 2016, 46(6): 339-343.

References

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Study of anti-irritation effect of hyaluronic acid using 3D skin model

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