表皮葡萄球菌发酵提取物对人体皮肤屏障的影响

doi:10.3969/j.issn.1001-1803.2022.04.006

Abstract

Abstract:

A large number of studies have shown that Staphylococcus epidermidis （SE）, one of the normal microflora colonized in human skin and mucous membrane, plays a positive role in human skin barrier health. Here we reveal a new mechanism which a product of Staphylococcus epidermidis can enhance the skin barrier function. This enhancement is mediated by a Staphylococcus epidermidis fermentation extract isolated from healthy human skin in our laboratory, and acts on keratinocytes （KC） as well as 3D epidermal model triggered through Toll-like receptor （TLR） 3, filaggrin （FLG） and loricin （LOR）, respectively. The effect of SE fermentation extract on human keratinocytes was detected by Real Time Cellular Analysis （RTCA）. Polyinosinic-polycytidylic acid （poly （I:C）） and lipopolysaccharide （LPS） were used to stimulate KC cells respectively, causing TLR3 and TLR4-mediated inflammatory response models, and then added SE fermentation extract to study the inhibitory effects of SE fermentation extract on the inflammatory KC cells. Finally, sodium lauryl sulfate （SLS） was used to stimulate the 3D epidermal model to construct a damaged skin model, and to study the effect of SE fermentation extract on the expression of filaggrin （FLG） and loricin （LOR） in the damaged skin model. The results show that 1% （φ） SE fermentation extract acts on KC cells for 100 hours, and no obvious cytotoxicity is seen, which has a certain protective effect on cell activity. From this we can predict that SE may have some effect on the function of human epidermis, for example, enhancing the skin barrier function. SE fermentation extract can inhibit the inflammatory response mediated by TLR3, but has no obvious inhibitory effect on the inflammatory response mediated by TLR4. We speculate that Staphylococcus epidermidis fermentation extract may affect the inflammatory response of keratinocytes through the negative regulation of TLR signal. The results of the 3D skin model show that the SE fermented extract can effectively increase the FLG and LOR content in the damaged barrier. These findings show that Staphylococcus epidermidis, as a resident bacteria of human skin, can modulate specific cutaneous inflammatory responses and enhance the skin barrier function through research on keratinocytes and 3D epidermal model after wounding.

Key words: Staphylococcus epidermidis, inflammatory cytokines, skin barrier, 3D skin model

CLC Number:

TQ658

Tie Hang,Lv Yufeng,Zhang Yang,Xu Liang,Yan Yan. Effect of Staphylococcus epidermidis fermented extract on human skin barrier[J].China Surfactant Detergent & Cosmetics, 2022, 52(4): 383-389.

Figures/Tables 7

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References 26

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相关蛋白	实验分组	相对IOD平均值	SD值	P值
FLG	空白对照	100.00%	10.50%	—
	阴性对照	17.57%	1.83%	<0
	阳性对照	45.68%	7.28%	0.003
	1%（φ） SE发酵粗提物	65.30%	4.96%	<0
LOR	空白对照	100.00%	10.59%	—
	阴性对照	13.01%	2.50%	<0
	阳性对照	64.40%	9.15%	0.001
	1%（φ） SE发酵粗提物	22.44%	2.01%	0.007

Effect of Staphylococcus epidermidis fermented extract on human skin barrier

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