柞蚕丝素肽脂质体的制备及其抗光损伤作用研究

doi:10.3969/j.issn.2097-2806.2026.01.009

Abstract

Abstract:

A preparation process of tussah silk fibroin peptide liposomes with high encapsulation efficiency and suitable particle size was established by the reverse rotary evaporation method, and their anti-photodamage effect was evaluated in this study. The results show that the optimal encapsulation effect of tussah silk fibroin peptide liposomes is achieved by optimizing the single factor test to ascertain the preparation parameters of the mass ratio of cholesterol to lecithin of 1∶11, the aqueous phase volume of 3 mL, the pH of PBS solution of 8.0, the first ultrasound of 3 min, the second ultrasound of 5 min, and the emulsification temperature of 40 ℃. The tussah silk fibroin peptide liposomes are capable of ameliorating the photodamage to mouse embryonic fibroblasts by repairing collagen, enhancing anti-oxidation and exerting anti-inflammatory effects. Ultraviolet damage of zebrafish is alleviated by reducing reactive oxygen species （ROS） levels and repairing the tail fin. This study confirms the potential of tussah silk fibroin peptide liposomes in resisting skin photodamage, thereby rendering them suitable for application in skin care products.

Key words: tussah silk fibroin peptide, liposome, antioxidant, anti-inflammatory, photodamage repair

CLC Number:

TQ658

Rui Mi, Xuejun Li, Linmei Wang, Xinghe Chen, Xinyue Jia, Xingfan Du. Study on the preparation of tussah silk fibroin peptide liposomes and their anti-photodamage effects[J].China Surfactant Detergent & Cosmetics, 2026, 56(1): 73-81.

Figures/Tables 7

Fig.1

Fig.2

Fig.3

Fig.4

Fig.5

Fig.6

Fig.7

References 23

[1]	Xing H, Pan X, Hu Y, et al. High molecular weight hyaluronic acid-liposome delivery system for efficient transdermal treatment of acute and chronic skin photodamage[J]. Acta Biomaterialia, 2024, 182: 171-187. doi: 10.1016/j.actbio.2024.05.026
[2]	何琪钰, 李雯琪, 李雨橦, 等. 紫外线对人体皮肤的光毒性效应及机制[J]. 生态毒理学报, 2024, 19(2): 66-74.
[3]	Parrado C, Mercado-Saenz S, Perez-Davo A, et al. Environmental stressors on skin aging mechanistic insights[J]. Frontiers in Pharmacology, 2019, 10: 759. doi: 10.3389/fphar.2019.00759 pmid: 31354480
[4]	Passeron T, Zouboulis C, Tan J, et al. Adult skin acute stress responses to shortterm environmental and internal aggression from exposome factors[J]. Journal of the European Academy of Dermatology and Venerology, 2021, 35(10): 1963-1975.
[5]	Rivas M, Calaf G M, Laroze D, et al. Solar ultraviolet A radiation and nonmelanoma skin cancer in Arica, Chile[J]. Journal of Photochemistry and Photobiology B: Biology, 2020, 212: 112047. doi: 10.1016/j.jphotobiol.2020.112047
[6]	米锐, 李学军, 马淑慧, 等. 响应面法优化水解制备柞蚕丝素肽及其性能[J]. 日用化学工业, 2021, 51(7): 612-619.
[7]	李学军, 米锐, 王林美, 等. 柞蚕丝素肽的抗氧化及美白功效评价[J]. 日用化学工业(中英文), 2023, 53(8): 891-619.
[8]	Zhang C, Lu Y, Ai Y, et al. Glabridin liposome ameliorating UVB-induced erythema and lethery skin by suppressing inflammatory cytokine production[J]. Journal of Microbiology and Biotechnology, 2021, 31(4): 630-636. doi: 10.4014/jmb.2011.11006 pmid: 33526759
[9]	Wang Y, Ouyang Q, Chang X, et al. Anti-photoaging effects of flexible nanoliposomes encapsulated Moringa oleifera Lam. isothiocyanate in UVB-induced cell damage in HaCaT cells[J]. Drug Delivery, 2022, 29(1): 871-881. doi: 10.1080/10717544.2022.2039802
[10]	吴琦. 抗氧化蛋清肽脂质体的制备及其产品的研发[D]. 长春: 吉林大学, 2018.
[11]	Xiang N, Huang Z, Zhang C, et al. Antiaging synergistic effect in noninvasive transdermal delivery of peptide loaded liposomes by low energy/frequency radiofrequency[J]. International Journal of Pharmaceutics: X, 2024, 8: 100289. doi: 10.1016/j.ijpx.2024.100289
[12]	Gromkowska-Kepka K J, Puscion-Jakubik A, Markiewicz-Zukowska R, et al. The impact of ultraviolet radiation on skin photoaging-review of in vitro studies[J]. Journal of Cosmetic Dermatology, 2021, 20: 3427-3431. doi: 10.1111/jocd.14033 pmid: 33655657
[13]	Liu C, Guo X, Chen Y, et al. Anti-photoaging effect and mechanism of flexible liposomes co-loaded with apigenin and doxycycline[J]. Biomedicine & Pharmacotherapy, 2023, 164: 114998. doi: 10.1016/j.biopha.2023.114998
[14]	Zhang R, Wei Y, Zhang J, et al. Protection effects of rice protein hydrolysate on UVB-irradiated photodamage in hartley guinea pigs skin and human skin fibroblasts[J]. Journal of Functional Foods, 2021, 82: 104504. doi: 10.1016/j.jff.2021.104504
[15]	Morresi C, Luccarini A, Marcheggiani F, et al. Modulation of paraoxonase-2 in human dermal fibroblasts by UVA-induced oxidative stress: a new potential marker of skin photodamage[J]. Chemico-Biological Interactions, 2023, 384: 10702.
[16]	Mostafa D K, Omar S I, Abdellatif A A, et al. Differential modulation of autophagy contributes to the protective effects of resveratrol and Co-Enzyme Q10 in photoaged mice[J]. Current Molecular Pharmacology, 2021, 14(3): 458-468. doi: 10.2174/1874467213666200730114547
[17]	Wang S, Yang M, Yin S, et al. A new peptide originated from amphibian skin alleviates the ultraviolet B-induced skin photodamage[J]. Biomedicine & Pharmacotherapy, 2022, 150: 112987. doi: 10.1016/j.biopha.2022.112987
[18]	Wang L, Oh J, Lee W, et al. Fucoidan isolated from Hizikia fusiforme suppresses ultraviolet B-induced photodamage by down-regulating the expressions of matrix metalloproteinases and pro-inflammatory cytokines via inhibiting NF-κB, AP-1, and MAPK signaling pathways[J]. International Journal of Biological Macromolecules, 2021, 166: 751-759. doi: 10.1016/j.ijbiomac.2020.10.232 pmid: 33144254
[19]	Sung M, Lee E, Jeon H, et al. Quercetin inhibits IL-1β-induced proliferation and production of MMPs, COX-2, and PGE2 by rheumatoid synovial fibroblast[J]. Inflammation, 2012, 35(4): 1585-1594. doi: 10.1007/s10753-012-9473-2
[20]	赖梓漩, 宋雨轩, 段雪伟, 等. 构树花粗多糖对紫外线诱导小鼠皮肤光损伤的保护作用及机制研究[J]. 日用化学工业(中英文), 2024, 54(9): 1069-1077.
[21]	Zang L, Shimada Y, Miyake H, et al. Transcriptome analysis of molecular response to UVC irradiation in zebrafish embryos[J]. Ecotoxicology and Environmental Safety, 2022, 231: 113211. doi: 10.1016/j.ecoenv.2022.113211
[22]	Chen B, Chen H, Qu H, et al. Photoprotective effects of Sargassum thunbergii on ultraviolet B-induced mouse L929 fibroblasts and zabrofish[J]. BMC Complementary Medicine and Therapies, 2022, 22: 144. doi: 10.1186/s12906-022-03609-x pmid: 35597942
[23]	Lee J H, Ko J Y, Kim E A, et al. Identification and large isolation of an anti-inflammatory compound from an edible brown seaweed, Undariopsis peterseniana, and evaluation on its anti-inflammatory effect in in vitro and in vivo zebrafish[J]. Journal of Applied Phycology, 2017, 29: 1587-1596. doi: 10.1007/s10811-016-1012-3

Study on the preparation of tussah silk fibroin peptide liposomes and their anti-photodamage effects

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 23

Related Articles 15

Metrics

Comments

Recommended 0

[1]	Min Liu, Lei Ye, Lingling Jiang, Jiuyan Zheng. Study on the repairing effect of ceramide liposomes on light injury and physical injury of skin [J]. China Surfactant Detergent & Cosmetics, 2025, 55(7): 902-908.
[2]	Hang Tie, Qi Li, Cuicui Zhu, Minjia Yuan, Liang Xu. Phosphatidylcholine-encapsulated resveratrol augments skin’s antioxidant and anti-wrinkle capabilities [J]. China Surfactant Detergent & Cosmetics, 2025, 55(6): 756-766.
[3]	Yuewen Ma, Xiaojing Li, Xuefeng Huang, Xian Chen, Chunli Wang. Protective effect and mechanism of Hedyotis diffusa extract on mice with solar dermatitis [J]. China Surfactant Detergent & Cosmetics, 2025, 55(5): 581-590.
[4]	Keran Feng, Xiaoming Wu, Liangbo Ma, Yu Sun. Determination of 21 nonsteroidal anti-inflammatory drugs in cosmetics by high performance liquid chromatography-tandem mass spectrometry [J]. China Surfactant Detergent & Cosmetics, 2025, 55(3): 399-406.
[5]	Ranran Dong,Yanhua Liu. Preparation of Moringa oleifera leaf ethanolic extract and study on its antibacterial and anti-inflammatory activities [J]. China Surfactant Detergent & Cosmetics, 2025, 55(2): 209-215.
[6]	Jianing Li, Shuyao Feng, Chun Zhang, Zhaohui Ge, Zhenshen Gao, Haijuan Zhang. Research progress of resveratrol in skin repair application [J]. China Surfactant Detergent & Cosmetics, 2025, 55(12): 1582-1588.
[7]	Wenlin Geng,Ming Li,Yuhua Cao. Studies on the antioxidant properties of Staphylococcus epidermidis fermentation broth [J]. China Surfactant Detergent & Cosmetics, 2025, 55(10): 1275-1283.
[8]	Xianxian Cao,Yonglei Yuan,Feifei Wang. Study on the antioxidant and anti-inflammatory activities of extracts from Salvia plebeia R. Br. [J]. China Surfactant Detergent & Cosmetics, 2025, 55(10): 1291-1298.
[9]	Qingyu Guo,Dan Cao,Dong Chen. Evaluation of the whitening effect and antioxidant activity of isorosmanol, an active ingredient of Rosmarinus officinalis L. [J]. China Surfactant Detergent & Cosmetics, 2025, 55(10): 1306-1312.
[10]	Youliang Zhou, Wanshan Lu, Zhiwei Cheng, Kun Dong. LC-MS/MS combined with network pharmacology and molecular docking to explore the targets and mechanisms of antioxidant effects of strawberry leaves [J]. China Surfactant Detergent & Cosmetics, 2025, 55(1): 63-74.
[11]	Chuanmao Li, Zhi Qiu, Fengliang Wang, Shengjie Lin, Hui Deng, Yingwei Li. Extraction and antioxidant activity of total flavonoids from chamomile [J]. China Surfactant Detergent & Cosmetics, 2024, 54(9): 1039-1049.
[12]	Zhiming Wei, Yaling Wang. Study and analysis of antibacterial and anti-inflammatory activity of extracts of Rhizoma imperatae in oral care products [J]. China Surfactant Detergent & Cosmetics, 2024, 54(9): 1086-1091.
[13]	Lin Sun, Man Zhang. Study on the anti-photoaging effect of Paulownia fortunei leaf extract [J]. China Surfactant Detergent & Cosmetics, 2024, 54(9): 1092-1098.
[14]	Wei Qian,Bin Yang,Bo Shao,Li Wan,Jun Zhou. Ultrasound-assisted enzymatic degradation to obtain pectin derivatives from Citrus aurantium L. and its antioxidant properties [J]. China Surfactant Detergent & Cosmetics, 2024, 54(8): 947-955.
[15]	Minjia Yuan,Qi Li,Cuicui Zhu,Hang Tie. Comparation of azelaic acid and chitosan encapsulated-azelaic acid complex in skin absorption efficiency and anti-inflammatory capability [J]. China Surfactant Detergent & Cosmetics, 2024, 54(8): 956-965.