复合多肽延缓小鼠皮肤衰老的功效研究

doi:10.3969/j.issn.2097-2806.2023.12.009

Abstract

Abstract:

Delaying skin aging has always been a hot topic in cosmetic research. In recent years, it has been reported that active peptides have the effect of delaying skin aging, thus research on substances that delaying skin aging is of great significance to the development of cosmetics and the application in the field of skin treatment. In the present study, we evaluated the anti-aging abilities of five bioactive peptides and the enhancing effect of the poly-peptide composition containing five bioactive peptides by four cell experiments and Elisa assay of cell proliferation, anti-oxidation, anti-inflammation, and promotion of collagen. Then, UVB radiation combined with D-galactose subcutaneous injection was adopted to establish animal skin aging model to investigate the anti-aging effects of the poly-peptide composition. Our data demonstrates that the five bioactive peptides have certain ability to promote cell proliferation, anti-oxidation, anti-inflammatory and promote collagen synthesis, and the poly-peptide composition have a significant enhancing effect in vitro. Importantly, after the administration of poly-peptide composition in vivo, the epidermal thickness decreases and tends to restore to normal （P<0.05）, and the contents of collagen and elastin increase, respectively （P<0.05）. Enzyme-linked immunosorbent assay results show that after the administration of poly-peptide composition, the expression of IL-1, IL-6, and TNF-α are down-regulated （P<0.05）, the expression of MMP3 and MMP9 is inhibited （P<0.05）, and the increased expression of β-galactosidase is significantly reduced （P<0.05）. The above results suggest that the composition of five bioactive peptide can play an anti-aging effect on the skin caused by UVB radiation combined with D-galactose subcutaneous injection.

Key words: bioactive peptide, anti-aging, D-galactose, anti-inflammatory, antioxidant, extracellular matrix, cell proliferation

CLC Number:

TQ658

Yani Xu, Kaiye Yang, Rongtao Zhu, Guangrong Liu, Ping Han, Zhiyun Du. Effects of compositions of bioactive peptides on skin aging in mice[J].China Surfactant Detergent & Cosmetics, 2023, 53(12): 1421-1428.

Figures/Tables 17

Tab. 1

Tab. 2

Tab. 3

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Fig. 8

Fig. 9

Fig. 10

Fig. 11

Fig. 12

Fig. 13

Fig. 14

References 16

[1]	Chatterjee C, Gleddie S, Xiao C W. Soybean bioactive peptides and their functional properties[J]. Nutrients, 2018, 10 (9) : 1211. doi: 10.3390/nu10091211
[2]	Jeong S, Yoon S, Kim S, et al. Anti-wrinkle benefits of peptides complex stimulating skin basement membrane proteins expression[J]. International Journal of Molecular Sciences, 2019, 21 (1) : 73. doi: 10.3390/ijms21010073
[3]	Choi Y L, Park E J, Kim E, et al. Dermal stability and in vitro skin permeation of collagen pentapeptides (KTTKS and palmitoyl-KTTKS)[J]. Biomolecules & Therapeutics, 2014, 22 (4) : 321-327.
[4]	Park H, An E, Cho Lee A R. Effect of palmitoyl-pentapeptide (pal-KTTKS) on wound contractile process in relation with connective tissue growth factor and α-smooth muscle actin expression[J]. Tissue Engineering and Regenerative Medicine, 2017, 14 (1) : 73-80. doi: 10.1007/s13770-016-0017-y pmid: 30603464
[5]	Choi S I, Lee J H, Kim J M, et al. Ulmus macrocarpa hance extracts attenuated H₂O₂ and UVB-induced skin photo-aging by activating antioxidant enzymes and inhibiting MAPK pathways[J]. International Journal of Molecular Sciences, 2017, 18 (6) : 1200. doi: 10.3390/ijms18061200
[6]	Choi H S, Park E D, Park Y, et al. Spent coffee ground extract suppresses ultraviolet B-induced photoaging in hairless mice[J]. Journal of Photochemistry and Photobiology B-Biology, 2015, 153: 164-172. doi: 10.1016/j.jphotobiol.2015.09.017
[7]	Pai V V, Bhandari P, Shukla P. Topical peptides as cosmeceuticals[J]. Indian Journal of Dermatology, 2017, 83 (1) : 9.
[8]	Schagen S. Topical peptide treatments with effective anti-aging results[J]. Cosmetics, 2017, 4 (2) : 16. doi: 10.3390/cosmetics4020016
[9]	Cabanos C, Matsuoka Y, Maruyama N. Soybean proteins/peptides: A review on their importance, biosynthesis, vacuolar sorting, and accumulation in seeds[J]. Peptides, 2021, 143: 170598. doi: 10.1016/j.peptides.2021.170598
[10]	Shin J, Kim J E, Pak K J, et al. A combination of soybean and haematococcus extract alleviates ultraviolet B-induced photoaging[J]. International Journal of Molecular Sciences, 2017, 18 (3) : 682. doi: 10.3390/ijms18030682
[11]	Pennington M W, Czerwinski A, Norton R S. Peptide therapeutics from venom: Current status and potential[J]. Bioorganic & Medicinal Chemistry, 2018, 26 (10) : 2738-2758. doi: 10.1016/j.bmc.2017.09.029
[12]	Balaev A N, Okhmanovich K A, Osipov V N. A shortened, protecting group free, synthesis of the anti-wrinkle venom analogue Syn-Ake® exploiting an optimized Hofmann-type rearrangement[J]. Tetrahedron Letters, 2014, 55 (42) : 5745-5747. doi: 10.1016/j.tetlet.2014.08.117
[13]	Fields K, Falla T J, Rodan K, et al. Bioactive peptides: signaling the future[J]. Journal of Cosmetic Dermatology, 2009, 8 (1) : 8-13. doi: 10.1111/j.1473-2165.2009.00416.x pmid: 19250159
[14]	Gorouhi F, Maibach H I. Role of topical peptides in preventing or treating aged skin[J]. International Journal of Cosmetic Science, 2009, 31 (5) : 327-345. doi: 10.1111/j.1468-2494.2009.00490.x pmid: 19570099
[15]	Duan X, Wu T, Liu T, et al. Vicenin-2 ameliorates oxidative damage and photoaging via modulation of MAPKs and MMPs signaling in UVB radiation exposed human skin cells[J]. Journal of Photochemistry and Photobiology B-Biology, 2019, 190: 76-85. doi: S1011-1344(18)31220-X pmid: 30502588
[16]	Tang S C, Liao P Y, Hung S J, et al. Topical application of glycolic acid suppresses the UVB induced IL-6, IL-8, MCP-1 and COX-2 inflammation by modulating NF-κB signaling pathway in keratinocytes and mice skin[J]. Journal of Dermatological Science, 2017, 86 (3) : 238-248. doi: 10.1016/j.jdermsci.2017.03.004

组别	处理方法
空白组	不照射UVB+100 μL生理盐水皮下注射，涂抹基质
模型组	照射UVB+100 μL 10% D-GAL皮下注射，涂抹基质
阳性组	照射UVB+100 μL 10% D-GAL皮下注射，涂抹基质+VE（1 mg/g）混合物
多肽复合组	照射UVB+100 μL 10% D-GAL皮下注射，涂抹基质+多肽复合混合物

多肽	动物实验质量浓度/（μg/mL）
五肽-3	61.875
棕榈酰三肽-5	27.5
类蛇毒肽	62.5
六肽-11	80
大豆多肽	35

试剂	w/%	功能定位
提取物	5	主药
角鲨烷	13	油相
Montanov S	7	表面活性剂
甘油	8	水相、保湿剂
尼泊金乙酯	0.1	防腐剂
蒸馏水	66.9	—

Effects of compositions of bioactive peptides on skin aging in mice

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 17

References 16

Related Articles 15

Metrics

Comments

Recommended 10

[1]	Yaoyao Li. Study on the anti-aging and antioxidant effects of isosinensetin [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 313-319.
[2]	Weilan Liu, Li Li, Chunnian He, Yinmao Dong, Xiao Ling, Miaomiao Guo. Study on the inhibitory effect of total glycosides of Paeonia veitchii and its main component paeoniflorin on acne-associated inflammation [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 188-195.
[3]	Xue Xiao, Huirong Zhang, Shuowen Li, Sunhua Li, Miaomiao Guo, Li Li. Determination of four polyphenols and study on antioxidant and anti-inflammatory activities of extracts from callus of Eryngium maritimum L. [J]. China Surfactant Detergent & Cosmetics, 2024, 54(1): 57-64.
[4]	Anna Wang, Mengjie Fang, Chao Tang, Tianxiang Yue. Protective effect and antioxidant mechanism of Danshensu on UVB-induced skin photoaging in mice [J]. China Surfactant Detergent & Cosmetics, 2024, 54(1): 65-72.
[5]	Chen Liyuan, Gao Heyi, Tang Jinjing, Liu Zuohua, Liu Renlong. Evaluation of antioxidant and protective effect against hair light damage of manganese compound [J]. China Surfactant Detergent & Cosmetics, 2023, 53(9): 1044-1050.
[6]	Fang Tinghuan, Zheng Ting, Jiang Qing, Li Xiaoxia, Tang Lirong. Synergistic effect of silk peptides thermophilus fermentation on the skin anti-inflammatory and anti-aging activities [J]. China Surfactant Detergent & Cosmetics, 2023, 53(9): 1057-1064.
[7]	Hao Qing, Wu Ermin, Bi Yongxian, Liu Jinjun, Kong Decheng, Cheng Zhiwei. Study on screening extraction process of total flavonoids from Nelumbinis Plumula by polyols and its properties [J]. China Surfactant Detergent & Cosmetics, 2023, 53(4): 398-407.
[8]	Ren Jiejie. Change in total flavonoid content of hawthorn leaves after adverse stress treatment and the molecular mechanism on myocardial ischemia in rats [J]. China Surfactant Detergent & Cosmetics, 2023, 53(4): 430-436.
[9]	Zhang Huirong, Guo Miaomiao, Chen Chen, Pan Qianyin, Zhang Ying, Li Li. The preparation process of three leguminous plant peptides and their activities [J]. China Surfactant Detergent & Cosmetics, 2023, 53(4): 423-429.
[10]	Liu Xiaoxing, Chen Chunyu, Li Li, Yi Fan. Research status and prospect of adaptogenic plants in skin health [J]. China Surfactant Detergent & Cosmetics, 2023, 53(3): 325-332.
[11]	Dai Liyun, Wei Yujie, Lu Zhen, Wang Yuling, Yan Huan, Wu Yue. Fermented brown rice filtrate: a substance with anti-aging potential [J]. China Surfactant Detergent & Cosmetics, 2023, 53(3): 292-299.
[12]	Li Huiling, Zhou Chunxia, Zhang Zhang. Study on skin care effects of Rosa graciliflora callus extract in human dermal fibroblast cells and 3D epidermal model [J]. China Surfactant Detergent & Cosmetics, 2023, 53(3): 300-307.
[13]	Deng Mengjie, Yi Guobin, Lv Ran, Liu Yafeng, Ye Dawei, Chen Jiazhi. Ferulic acid nanoethosomes: preparation, characterization, and performance of skin penetration in vitro [J]. China Surfactant Detergent & Cosmetics, 2023, 53(11): 1285-1292.
[14]	Zhou Yan, Han Ruina. Study on the skincare efficacy and safety evaluation of Rhodiola rosea extract [J]. China Surfactant Detergent & Cosmetics, 2023, 53(10): 1180-1185.
[15]	Zhong Meiying, Zhang Hao, Huang Qin, Tang Wendi, Lan Ailing. Research and development of skin anti-aging and related cosmetics based on epigenetics [J]. China Surfactant Detergent & Cosmetics, 2023, 53(10): 1220-1226.