酶水解法制备黄芪素及应用研究

doi:10.3969/j.issn.2097-2806.2023.05.008

Abstract

Abstract:

This research investigated the preparation of astragalin by enzymatic hydrolysis method and its application. Using the extraction rate of Astragalus as the evaluation index, astragalin was extracted by enzymatic hydrolysis method and determined by using high performance liquid chromatography （HPLC）. By constructing aging model mice, the astragalin prepared by enzymatic hydrolysis method was applied to the aging model mice, and the learning and memory ability and the oxidation level of brain tissue of the aging model mice were observed. The results show that the orthogonal test of enzymatic hydrolysis method is superior when the material-liquid ratio is 1∶10, the number of extractions is 3 times, the extraction temperature is 80 ℃ and the extraction time is 1 min, with the highest extraction rate of Astragalus. Compared with the control group, astragalin significantly improves the learning and memory abilities of the aging model mice （P<0.05）. The contents of superoxide dismutase （SOD） and catalase （CAT） in the brain tissues of the aging model mice increase significantly after treatment with astragalin （P<0.05）, while the contents of malondialdehyde （MDA） decrease significantly （P<0.05）. In this study, by optimising the preparation method of Astragalus and testing the anti-ageing ability of Astragalus, the study lays the theoretical and practical foundation for the further development and clinical application of Astragalus in the future.

Key words: enzymatic hydrolysis, Astragalus, aging model mice, anti-oxidation, application research

CLC Number:

TQ658

Qi Guofeng, Li Ganggang, An Yan. Study on the preparation of Astragalus by enzymatic hydrolysis and its application[J].China Surfactant Detergent & Cosmetics, 2023, 53(5): 544-550.

Figures/Tables 10

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References 19

[1]	Wang Xiaoju. Extraction, separation and activity study of the active components of Astragalus[D]. Xi’an: Shanxi University, 2005.
[2]	Wang Guangzhong, Hu Di, Chen Jingbing. Orthogonal design to optimize the extraction process of total flavonoids and total saponins of Astragalus[J]. Chinese Patent Medicine, 2004, 26(10): 3-4.
[3]	Liu Jinhua, Chen Jingran, You Guangfu. Advances in pharmacological research on myocardial protection of Astragalus[J]. Chinese Patent Medicine, 2002, 24(8): 623-626.
[4]	Guo Wei. Pharmacology and clinical research overview of traditional Chinese medicine Astragalus[J]. Shanxi Traditional Chinese Medicine, 2011, 27(11): 52-54.
[5]	Lu Yanqi, He Xueli. A review of the chemical constituents and pharmacological effects of Astragalus[J]. Journal of Baoding Teachers College, 2004, 17(4): 40-42.
[6]	Li Qin, Liu Hong. Pharmacological effects and clinical application of Astragalus[J]. Chinese Medicine of Traditional Chinese Medicine, 2004, 10(10): 633-635.
[7]	Jin Jizi. Research progress on the pharmacological effects and clinical application of Astragalus[J]. Chinese Medicine of Traditional Chinese Medicine, 2008, 17(20): 134-135.
[8]	Chen Jianzhen, Lv Guiyuan, Ye Lei. Research progress on chemical constituents and pharmacological effects of Astragalus flavonoids[J]. Medical Herald, 2009, 28(10): 1314-1316.
[9]	Liang Liansheng, Yu Jing. Pharmacological effects of flavonoids in Astragalus[J]. Journal of Integrative Chinese and Western Medicine Cardiovascular and Cerebrovascular Diseases, 2005, 3(12): 1085-1087.
[10]	Zhang Dongqing, Wang Deqing. Research progress on biological activity of total flavonoids of Astragalus[J]. China Important Journal, 2010, 35(2): 253-256.
[11]	Li Y, Guo S, Zhu Y, Yan H, et al. Flowers of Astragalus membranaceus var. mongholicus as a novel high potential by product: phytochemical characterization and antioxidant activity[J]. Molecules, 2019, 24(3): 434. doi: 10.3390/molecules24030434
[12]	Butkutė B, Dagilytė A, Benetis R, et al. Mineral and phytochemical profiles and antioxidant activity of herbal material from two temperate Astragalus species[J]. Biomed. Res. Int., 2018.
[13]	Sharifi-Rad M, Pohl P, Epifano F, et al. Green synthesis of silver nanoparticles using Astragalus tribuloides delile. Root extract: characterization, antioxidant, antibacterial, and anti-Inflammatory activities[J]. Nanomaterials (Basel), 2020, 10(12): 2383. doi: 10.3390/nano10122383
[14]	Wang D, Zhuang Y, Tian Y, et al. Study of the effects of total flavonoids of Astragalus on atherosclerosis formation and potential mechanisms[J]. Oxid. Med. Cell. Longev., 2012.
[15]	Jiao J, Gai QY, Wang W, et al. Ultraviolet radiation-elicited enhancement of isoflavonoid accumulation, biosynthetic gene expression, and antioxidant activity in Astragalus membranaceus hairy root cultures[J]. J. Agric. Food. Chem., 2015, 63(37): 8216-8224. doi: 10.1021/acs.jafc.5b03138
[16]	Li Z P, Liu H B, Zhang Q W, et al. Interference of quercetin on Astragalus polysaccharide-induced macrophage activation[J]. Molecules, 2018, 23(7): 1563. doi: 10.3390/molecules23071563
[17]	Alblihed M A. Astragalin attenuates oxidative stress and acute inflammatory responses in carrageenan-induced paw edema in mice[J]. Mol. Biol. Rep., 2020, 47(9): 6611-6620. doi: 10.1007/s11033-020-05712-z
[18]	Petreanu M, Maia P, da Rocha P J L, et al. Antidepressant-like effect and toxicological parameters of extract and withanolides isolated from aerial parts of Solanum capsicoides All. (Solanaceae)[J]. Naunyn Schmiedebergs Arch. Pharmacol., 2019, 392(8): 979-990. doi: 10.1007/s00210-019-01648-9
[19]	Ceramella J, Loizzo M R, Iacopetta D, et al. Anchusa azurea Mill. (Boraginaceae) aerial parts methanol extract interfering with cytoskeleton organization induces programmed cancer cells death[J]. Food Funct., 2019, 10(7): 4280-4290. doi: 10.1039/c9fo00582j pmid: 31264668

水平	A 提取温度/℃	B 提取时间/min	C 料液比/（g∶mL）	D 提取次数/次
1	60	1	1∶12	1
2	70	3	1∶10	2
3	80	5	1∶ 8	3

组号	D	C	B	A	黄芪素含量/（mg/g）
1	1	1	1	1	1.590 7
2	1	2	2	2	1.629 3
3	1	3	3	3	1.436 0
4	2	1	2	3	2.117 5
5	2	2	3	1	2.279 7
6	2	3	1	2	2.302 4
7	3	1	3	2	2.890 6
8	3	2	1	3	2.940 1
9	3	3	2	1	2.912 4
均值1	1.498	2.103	2.146	2.153
均值2	2.187	2.170	2.118	2.137
均值3	2.806	2.102	2.193	2.161
极差R	1.243	0.076	0.035	0.063

Study on the preparation of Astragalus by enzymatic hydrolysis and its application

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