Abstract:

High performance gel filtration chromatography was used to determine the polysaccharide components in sapiens. The hydrogel column Ultra-hydrogel^™ Linear 300 mm×7.8 mmid×2 was used as the separation column with the mobile phase of 0.1mol/L sodium nitrate, the flow rate of 0.8 mL/min, and the column temperature at 40 ℃. Test results show that the polysaccharide components 1# （M_w=2 115 637）, 2#（M_w=12 516）, 3#（M_w=418） are in the range of 0.10-5.00 g/L, the peak areas and mass concentrations appear good linear correlation with the correlation coefficient γ of 0.999 5, 0.999 2, and 0.999 0（n=5）, respectively. The detection limits are 0.056, 0.053, and 0.050 g/L, respectively, the recoveries of this method are 96.7%-102.0%, 96.0%-101.6%, and 94.0%-103.6%, respectively, and the relative standard deviations are 0.63%-1.81%, 0.81%-1.94%, and 1.01%-2.03%（n=6）, respectively. The method is simple, has good repeatability and recovery, and can be used for the determination of polysaccharide components in sapiens.

Key words: Gleditsia sinensis seed, high performance gel filtration chromatography, polysaccharide