Abstract: A HPLC method for simultaneous determination of contents of monose and polysaccharides in glucoside-based surfactant products was established.The method adopts evaporative light-scattering detector and Zorbax Eclipse Plus C₁₈ column (4.6 mm×100 mm,3.5 μm) with methanol and water as the mobile phase at a rate of 1.0 mL/min.The contents of saccharides were calculated by external standard two-point method using glucose and maltotriose as control samples.Results show that the detection limit of monose and polysaccharides is 1.5 and 2.0 μg/L respectively while the recovery is 100.4% and 99.1% respectively.As comparing with the conventional methods,this method displays unique superiority in determination of non-reducing monose and the residual glucose from raw material for preparation of glucoside-based surfactants as well as the by-products polysaccharides can be determined simultaneously.

Key words: glucoside-based surfactant, monose, polysaccharides, HPLC