基于酶催化合成单甘油酯工艺优化及抑菌活性研究

doi:10.3969/j.issn.1001-1803.2023.02.008

Abstract

Abstract:

Lauric acid monoglyceride （GML） was synthesized, in which lauric acid and glycerol were used as raw materials, tert-butanol was used as the medium, and lipase was used to catalyze the reaction. Single-factor experiment was used to optimize the synthesis process of GML. The optimized conditions were as follows: The mass ratio of tert-butanol to glycerol was 1.5∶1, the amount of lipase was 12%, the reaction temperature was 70 ℃, the molar ratio of glycerol to lauric acid was 3∶1, and the reaction time was 1.5 h. The antibacterial test showed that GML had significant inhibitory effect against Escherichia coli, and the antibacterial effect was increased with the increase of mass concentration. The diameter of inhibition zone would not be further increased when the mass concentration of GML reached 16 mg/mL. The antibacterial effect and the half-life of bacteriostatic rate in the faintly acidic environment of pH 5.7 were both stronger than that in the neutral condition of pH 7.2, and the bacteriostatic rate of GML against Escherichia coli was still maintained at 3.9% in the environment of pH 5.7 at 72 h.

Key words: enzyme catalysis, lipase, lauric acid monoglyceride （GML）, synthesis process, bacteriostatic effect

CLC Number:

TQ658

Li Xiaomin. Process optimization of enzyme-catalyzed synthesis of monoglyceride and its antibacterial activity[J].China Surfactant Detergent & Cosmetics, 2023, 53(2): 180-186.

Figures/Tables 9

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References 19

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试验	脂肪酶用量/%	反应温度/℃	甘油与月桂酸摩尔比	反应时间/h	GML 含量/%
1	8	50	1∶1	0.5	50.3
2	8	60	2∶1	1	55.7
3	8	70	3∶1	1.5	65.8
4	8	80	4∶1	2	63.5
5	10	50	2∶1	1	60.9
6	10	60	1∶1	0.5	64.9
7	10	70	3∶1	2	75.8
8	10	80	4∶1	1.5	67.2
9	12	50	1∶1	0.5	70.2
10	12	60	4∶1	1	78.8
11	12	70	3∶1	1.5	83.1
12	12	80	2∶1	2	75.2
13	14	50	4∶1	2	67.1
14	14	60	3∶1	1.5	70.5
15	14	70	2∶1	1	66.2
16	14	80	1∶1	0.5	63.7
均值1	58.825	62.125	62.275	62.275
均值2	67.2	67.475	64.5	65.65
均值3	76.825	72.725	73.8	71.65
均值4	66.875	67.4	69.15	70.4
极差	18.0	10.6	11.525	9.375

Process optimization of enzyme-catalyzed synthesis of monoglyceride and its antibacterial activity

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