Abstract:

Network pharmacology was used to explore the anti-inflammatory and antibacterial mechanism of basil, which was preliminarily verified by in vitro test. Through the relevant database, 18 compounds and 6 volatile oil components of basil are screened, and 521 action targets, 975 anti-inflammatory and antibacterial targets are obtained. The Venny 2.1 is used to make the Wayne diagram of the intersection of drugs and disease targets, and 88 basil anti-inflammatory and antibacterial targets are obtained. The protein-protein interaction PPI network is constructed and enriched by go and KEGG. According to the protein correlation degree, the core target involving TNF-α, IL-1 β and IL-6 plays an anti-inflammatory and antibacterial role mainly through the signal pathways such as response to bacteria and response to lipopolysaccharide. In vitro, two inflammatory cell models of THP-1 induced by LPS and heat inactivated Cutibacterium acnes （C.a） were used to detect TNF in cells supplemented with different concentrations of Ocimum basilicum L. essential oil （OBEO） by TNF-α, IL-1 β, IL-6 secretion. The results show that OBEO at a volume fraction of 0.025% is able to inhibit the expression of TNF-α, IL-1β, and IL-6 in both inflammatory injury models. And the inhibitory effect of OBEO on inactivated C.a caused inflammatory injury in THP-1 cells is significant, reducing the expression of TNF-α, IL-1β, and IL-6 by about 24%, 45%, and 13%, respectively.

Key words: Ocimum basilicum L., anti-inflammatory and antibacterial, network pharmacology, THP-1, pathway