山茶油对D-半乳糖致衰老小鼠皮肤抗衰老功效的代谢组学研究

doi:10.3969/j.issn.1001-1803.2021.07.008

Abstract

Abstract:

The aim of the study was to explore the anti-aging effect and physiological mechanism of Camellia seed oil and Camellia emollient oil on the skin of D-galactose-induced aging mice. The skin tissues of mice in normal group, model group, Camellia seed oil group, Camellia emollient oil group and vitamin E group （positive control） were analyzed by gas chromatography time-of-flight mass spectrometry （GC-TOF/MS）. After obtaining the data, the multidimensional statistical method was used to analyze the data and to mine the biological information. The data were analyzed by the mathematical model of principal component analysis （PCA） and orthogonal projections to latent structures discriminant analysis （OPLS-DA）, and the differential metabolites were determined by the indices of variable importance in the projection （VIP） andp value. The differential metabolites which were filtered out were substituted into the MetaboAnalyst platform, and the important metabolic pathways involved were screened by indices of pathway impact and p value. The analysis of differential metabolites shows that the differential metabolites between normal group and model group are propionic acid, linoleic acid, aminomalonic acid, aspartic acid, tyrosine, succinic acid, stearic acid, alanine and proline; the differential metabolite between model group andCamellia seed oil group is palmitic acid; the differential metabolites between model group and Camelliaemollient oil group are propionic acid, succinic acid, aminomalonic acid, stearic acid, fumaric acid, proline, linoleic acid and alanine; the differential metabolites between model group and vitamin E group are propionic acid, succinic acid, aminomalonic acid, stearic acid, linoleic acid, aspartic acid, proline and phosphoric acid. Camellia seed oil and Camellia emollient oil can regulate the metabolism disorder of the skin of D-galactose-induced aging mice. From the perspective of the number of differential metabolites, Camellia emollient oil is better than Camelliaseed oil, close to vitamin E in the anti-aging effect of skin. Metabolic pathway analysis indicates three major metabolic pathways: alanine, aspartic acid, and glutamic acid metabolism, phenylalanine, tyrosine, and tryptophan metabolism, and linoleic acid metabolism. Through further analysis, it is speculated that the effect of Camellia emollient oil andCamellia seed oil may be related to the regulation of oxidative stress. This research result may provide a reference for further study revealing the anti-aging mechanism of Camellia oil.

Key words: Camellia seed oil, Camelliaemollient oil, skin aging, metabolomics, differential metabolites, metabolic pathway

CLC Number:

TQ658

Wu Yani,Tang Yin,Wang Shuchang,Wang Ying,Lv Xiaofan. Metabolomics study of anti-aging effect of Camelliaoil on the skin of D-galactose-induced aging mice[J].China Surfactant Detergent & Cosmetics, 2021, 51(7): 632-638.

Figures/Tables 11

Fig. 1

Fig. 2

Fig. 3

Tab. 1

Fig. 4

Fig. 5

Tab. 2

Fig. 6

Tab. 3

Fig. 7

Tab. 4

References 27

[1]	Trivedi D K, Hollywood K A, Goodacre R. Metabolomics for the masses: the future of metabolomics in a personalized world[J]. New Horizons in Translational Medicine, 2017,3(6) : 294-305. doi: 10.1016/j.nhtm.2017.06.001 pmid: 29094062
[2]	Yang Qiang, Zhang Aihua, Miao Jianhua, et al. Metabolomics biotechnology, applications, and future trends: a systematic review[J]. RSC Advances, 2019,9(64) : 37245-37257. doi: 10.1039/c9ra06697g
[3]	Liu Yumin, Chen Tianlu, Qiu Yunpin, et al. An ultrasonication-assisted extraction and derivatization protocol for GC/TOFMS-based metabolite profiling[J]. Analytical and Bioanalytical Chemistry, 2011,40(5) : 1405-1417.
[4]	Rainville P D, Stumpf C L, Shockcor J P, et al. Novel application of reversed-phase UPLC-oaTOF-MS for lipid analysis in complex biological mixtures: a new tool for lipidomics[J]. Journal of Proteome Research, 2007,6(2) : 552-558. pmid: 17269712
[5]	Worley B, Powers R. PCA as a practical indicator of OPLS-DA model reliability[J]. Current Metabolomics, 2016,4(2) : 97-103. doi: 10.2174/2213235X04666160613122429
[6]	Chong J, Soufan O, Li C, et al. MetaboAnalyst 4.0: towards more transparent and integrative metabolomics analysis[J]. Nucleic Acids Research, 2018,46(1) : 486-494.
[7]	Zeng W, Endo Y. Lipid characteristics of Camellia seed oil[J]. Journal of Oleo Science, 2019,68(7) : 649-658. doi: 10.5650/jos.ess18234 pmid: 31178460
[8]	Lee W T, Tung Y T, Wu C C, et al. Camellia oil (Camellia oleifera abel.) modifies the composition of gut microbiota and alleviates acetic acid-induced colitis in rats[J]. Journal of Agricultural and Food Chemistry, 2018,66(28) : 7384-7392. doi: 10.1021/acs.jafc.8b02166
[9]	Lee C P, Yen G C. Antioxidant activity and bioactive compounds of tea seed (Camellia oleifera Abel.) oil[J]. Journal of Agricultural and Food Chemistry, 2006,54(3) : 779-784. doi: 10.1021/jf052325a
[10]	Feas X, Estevinho L M, Salinero C, et al. Triacylglyceride, antioxidant and antimicrobial features of virgin Camellia oleifera, C. reticulata and C. sasanqua oils[J]. Molecules, 2013,18(4) : 4573-4587. doi: 10.3390/molecules18044573
[11]	Wang Shuchang, Wu Yani. Effect of Camellia oil on the skin of D-galactose induced aging mice model[J]. China Surfactant Detergent & Cosmetics, 2019,49(5) : 320-327.
[12]	Liu Yumin, Wei Zhenyu, Chen Xin, et al. Metabonomic study of ischemic stroke based on GC-TOFMS approach[J]. Journal of Shanghai Jiaotong University (Medical Science), 2017,37(2) : 207-211.
[13]	Shui Sufang, Cai Xiaorong, Huang Rongqing, et al. The investigation of anti-inflammatory activity of Yi Guanjian decoction by serum metabonomics approach[J]. Journal of Pharmaceutical and Biomedical Analysis, 2017,133:41-48. doi: S0731-7085(16)31098-6 pmid: 27856104
[14]	Rahman S, Bhatia K, Khan A Q, et al. Topically applied vitamin E prevents massive cutaneous inflammatory and oxidative stress responses induced by double application of 12-O-tetradecanoylphorbol-13-acetate (TPA) in mice[J]. Chemico-Biological Interactions, 2008,172(3) : 195-205. doi: 10.1016/j.cbi.2007.11.017
[15]	Huang Zirou, Lin Yinku, Fang Jiayou. Biological and pharmacological activities of squalene and related compounds: potential uses in cosmetic dermatology[J]. Molecules, 2009,14(1) : 540-554. doi: 10.3390/molecules14010540
[16]	Barbosa E, Faintuch J, Machado-Moreira E A, et al. Supplementation of vitamin E, vitamin C, and zinc attenuates oxidative stress in burned children: a randomized, double-blind, placebo-controlled pilot study[J]. Journal of Burn Care & Research, 2009,30(5) : 859-866.
[17]	Poljšak B, Dahmane R G, Godić A. Intrinsic skin aging: the role of oxidative stress[J]. Acta dermatovenerologica Alpina, Panonica, Et Adriatica, 2012,21(2) : 33-36.
[18]	Qian Wen, Luo Dan, Zhou Bingrong. Research progress of the anti-ageing mechanism of carnosine[J]. Journal of Practical Dermatology, 2018,11(6) : 360-363.
[19]	Wu H C, Shiau C Y, Chen H M, et al. Antioxidant activities of carnosine, anserine, some free amino acids and their combination[J]. Journal of Food and Drug Analysis, 2003,11(2) : 148-153.
[20]	Smith A E, Stout J R, Kendall K L, et al. Exercise-induced oxidative stress: the effects of β-alanine supplementation in women[J]. Amino Acids, 2012,43(1) : 77-90. doi: 10.1007/s00726-011-1158-x pmid: 22102056
[21]	Liu Jiji. Research on aspartic acid inhibiting the browning of fresh-cut potatoes[D]. Taian: Shandong Agriculture University, 2019.
[22]	Leng Weibo, Liu Yulan, Li Shuang, et al. Effects of aspartic acid on intestinal morphology and mucosal antioxidant capacity of weaned piglets stimulated by lipopolysaccharide[J]. Chinese Journal of Animal Science, 2014,50(11) : 32-36.
[23]	Yen G C, Hsieh C L. Antioxidant effects of dopamine and related compounds[J]. Bioscience, Biotechnology, and Biochemistry, 1997,61(10) : 1646-1649. pmid: 10336274
[24]	Riley P A. Melanin[J]. International Journal of Biochemistry & Cell Biology, 1997,29(11) : 1235-1239. doi: 10.1016/S1357-2725(97)00013-7
[25]	Wang Huanjun, Liu Ana, Zhao Wenxiao, et al. Metabolomics research reveals the mechanism of action of astragalus polysaccharide in rats with digestive system disorders[J]. Molecules, 2018,23(12) : 1-14. doi: 10.3390/molecules23010001
[26]	Philipsen M H, Samfors S, Malmberg P, et al. Relative quantification of deuterated omega-3 and-6 fatty acids and their lipid turnover in PC12 cell membranes using TOF-SIMS[J]. Journal of Lipid Research, 2018,59(11) : 2098-2107. doi: 10.1194/jlr.M087734 pmid: 30206182
[27]	Patwardhan A M, Akopian A N, Ruparel N B, et al. Heat generates oxidized linoleic acid metabolites that activate TRPV1 and produce pain in rodents[J]. Journal of Clinical Investigation, 2010,120(5) : 1617-1626. doi: 10.1172/JCI41678

代谢物名称	VIP	p	变化趋势
丙酸	1.58	1.64×10^-4	↑
亚油酸	1.44	4.50×10^-3	↑
氨基丙二酸	1.40	1.11×10^-2	↑
天冬氨酸	1.37	4.09×10^-3	↑
酪氨酸	1.32	2.74×10^-3	↑
琥珀酸	1.12	4.00×10^-2	↑
硬脂酸	1.10	1.63×10^-2	↑
丙氨酸	1.06	2.05×10^-2	↑
脯氨酸	1.05	9.35×10^-3	↑

代谢物名称	VIP	p	变化趋势
丙酸	1.47	3.40×10^-5	↓
琥珀酸	1.45	3.50×10^-5	↓
氨基丙二酸	1.36	7.44×10^-4	↓
硬脂酸	1.33	7.69×10^-4	↓
延胡索酸	1.13	4.36×10^-3	↓
脯氨酸	1.10	1.46×10^-2	↓
亚油酸	1.09	3.65×10^-2	↓
丙氨酸	1.03	4.28×10^-2	↓

代谢物名称	VIP	p	变化趋势
丙酸	1.33	3.00×10^-6	↓
琥珀酸	1.28	3.20×10^-5	↓
氨基丙二酸	1.25	1.73×10^-4	↓
硬脂酸	1.24	9.30×10^-5	↓
亚油酸	1.15	1.02×10^-3	↓
天冬氨酸	1.12	2.09×10^-2	↓
脯氨酸	1.09	2.85×10^-3	↓
磷酸	1.07	9.21×10^-3	↑

代谢通路	p	-log（p）	pathway impact
丙氨酸、天冬氨酸和谷氨酸代谢	1.38×10^-2	4.28	0.19
苯丙氨酸、酪氨酸和色氨酸代谢	3.07×10^-2	3.48	0.50
亚油酸代谢	4.58×10^-2	3.08	1.00

Metabolomics study of anti-aging effect of Camelliaoil on the skin of D-galactose-induced aging mice

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 11

References 27

Related Articles 5

Metrics

Comments

Recommended 10

[1]	Du Kesi,Li Zeqiao,Zhang Baojiang,Zheng Yumei,Ren Hankun,Cheng Jianhua. Appearance changes and formation factors of facial skin aging [J]. China Surfactant Detergent & Cosmetics, 2022, 52(2): 199-206.
[2]	Jiang Dandan,Wang Fenglou,Li Hui,Zhang Zhang. Promotion effects of Dragon’s Blood Complex on mitochondrial network and bioenergetics in normal human skin cells [J]. China Surfactant Detergent & Cosmetics, 2021, 51(7): 626-631.
[3]	Wu Zichao,Ye Chao,Piao Mingguan. Preparation of diol-type ginsenoside W/O nanoemulsion and research on anti-aging mechanism of skin [J]. China Surfactant Detergent & Cosmetics, 2021, 51(10): 963-968.
[4]	ZHOU Chun-xia,ZHOU Li-dan,LU Yi-na. Study on the anti-aging efficacy of the palmitoyl oligopeptide composition [J]. China Surfactant Detergent & Cosmetics, 2020, 50(4): 263-268.
[5]	LI Cheng-tong, ZHAO Hua. Efficacy evaluation of cosmetics (Ⅳ) Scientific support for delaying skin aging cosmetic efficacy claims [J]. China Surfactant Detergent & Cosmetics, 2018, 48(4): 188-195.