Abstract:

Using oats as a culture medium, the β-glucan in oats was extracted by a bidirectional fermentation method, and its efficacy of cosmetic skin care was studied. Three fungi, i.e., Pholiota adiposa, Clitocybe maxima and Grifola frondose, were used for bidirectional fermentation with oats. The experimental results showed that the cytotoxicity of oats is significantly reduced after fermentation. By measuring the content of reactive oxygen species （ROS）, type I collagen （Col I） and matrix metalloproteinase （MMP-1） in fibroblasts, it was found that the fermentation broth had certain anti-aging effect, and the effect was better than that of unfermented oats. Compared with those before fermentation, the fermentation broth and purified fermented oat β-glucan showed relatively reduced content of active oxygen, relatively high content of Col I, and relatively low content of MMP-1in fibroblasts. By measuring the melanin content and tyrosinase activation rate in mouse melanoma cells（B16 cells）, it was found that the melanin content in the cells after being treated with the fermentation broth was lower, and the Pholiota adiposa/oat fermentation broth had the best effect. Franz diffusion cell transdermal absorption experiments had showed that the cumulative permeation amount of the fermentation broth is higher.

Key words: bidirectional fermentation, oat β-glucan, medicinal fungi, anti-aging, whitening, transdermal absorption