Abstract:

The aim of this study was to find the best synergistic anti-inflammatory ratio of Modified Jiedu Xiaozheng Yin （MJXY）, and to explore its anti-inflammatory effect and related mechanisms through lipopolysaccharide （LPS）-induced RAW264.7 macrophages in vitro. The anti-inflammatory and antioxidant activities of different extracted components in MJXY were evaluated by the scavenging rates of nitric oxide （NO） and 2, 2’-azinobis-（3-ethylbenzthiazoline-6-sulphonate） （ABTS）. According to the synergistic effect scores, the best ABTS （m（Scleromitrion diffusum）:m（Pyrola calliantha）:m（Prunella vulgaris）:m（Sophora flavescens）=20:5:6:6） and NO scavenging formulation （m（Scleromitrion diffusum）:m（Pyrola calliantha）:m（Prunella vulgaris）:m（Sophora flavescens）=12:12:4:3） were obtained. It is found that MJXY can significantly reduce the level of reactive oxygen species in cells, and inhibit the secretion of inflammatory cytokines. Furthermore, western blot experiments show that MJXY can exert anti-inflammatory effects by down-regulating the phosphorylation levels of JAK2 and STAT3 in the inflammatory signaling pathway. In summary, MJXY has a good anti-inflammatory effect and can improve skin inflammation by inhibiting the JAK2/STAT3 signaling pathway.

Key words: Jiedu Xiaozheng Yin, RAW264.7 cell, skin inflammation, JAK2/STAT3 signaling pathway