超声辅助酶法降解枳实果胶及其衍生物抗氧化性能

doi:10.3969/j.issn.2097-2806.2024.08.009

Abstract

Abstract:

In this paper, pectin derivatives obtained from the degradation of Citrus aurantium L. pectin was prepared by ultrasound-assisted enzymatic method. The effects of ultrasound pretreatment time, enzyme type and amount of enzyme addition on the antioxidant activity of degradation derivatives of Citrus aurantium L. pectin were investigated. Then the content, molecular weight distribution and structural properties of galacturonic acid from the degradation derivatives of Citrus aurantium L. pectin were analyzed by titration gel chromatography and infrared spectroscopy to verify the plausible explanation for the enhanced antioxidant activity of pectin degradation derivatives. The results confirm that the best scavenging rate of DPPH radicals and ABTS radicals as well as the reducing ability of potassium ferricyanide of the pectin degradation derivatives are obtained, when pectinase is selected as the degrading enzyme with the dosage of 6 000 U/g and the ultrasound-pretreatment time is 20 min. Under these conditions, the degradation derivatives of Citrus aurantium L. pectin prepared at a mass concentration of 3 mg/mL, shows a more significant increasing in antioxidant activity with a 62.94% scavenging of DPPH radicals, a 52.67% scavenging of ABTS radicals and a reducing capacity of 0.962 7, compared to the undegraded pectin. The content of galacturonic acid in the degradation derivatives of Citrus aurantium L. pectin varies significantly with the degradation process. Also it is verified that it is a positive correlation between galacturonic acid content and antioxidant activity, and that a lower molecular weight and lower methoxy content of the degradation derivatives are obtained compared to Citrus aurantium L. pectin, but the pyranose structure of the pectin is not changed in the degradation process. The results of this study provide theoretical basis and reference for the high-value utilization of Citrus aurantium L. pectin.

Key words: Citrus aurantium L. pectin, ultrasound assisted enzyme method, degradation derivatives, antioxidant activity, constitutive analysis

CLC Number:

TQ658

Wei Qian,Bin Yang,Bo Shao,Li Wan,Jun Zhou. Ultrasound-assisted enzymatic degradation to obtain pectin derivatives from Citrus aurantium L. and its antioxidant properties[J].China Surfactant Detergent & Cosmetics, 2024, 54(8): 947-955.

Figures/Tables 6

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Ultrasound-assisted enzymatic degradation to obtain pectin derivatives from Citrus aurantium L. and its antioxidant properties

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