茶麸黄酮促进毛乳头细胞增殖和VEGF分泌的研究

doi:10.3969/j.issn.1001-1803.2022.01.007

Abstract

Abstract:

Defatted camellia seed cake was used as raw material, and the flavonoids therein were extracted with aqueous ethanol solution with the aid of ultrasonic wave to explore their biological activity. Different mass concentrations of camellia seed cake flavonoids were applied to human dermal papilla cells （HDPCs） cultured in vitro, and the cell viability was determined by MTT method. The effects on apoptosis and cell cycle were detected by flow cytometry. ELISA method was used to detect the change of vascular endothelial growth factor （VEGF） in cell culture supernatant. The results showed that flavonoid-enriched product of camellia seed cake had a proliferation effect on HDPCs and could promote the secretion of VEGF in a concentration-dependent manner. The proliferation effect was the best when the concentration of flavonoid-enriched product was 20 μg/mL, which was more effective than the positive control of 10 μg/mL minoxidil （^**P<0.01）. The flavonoids in camellia seed cake might reduce the premature apoptosis of HDPCs and promote the mitosis of HDPCs and thus lead to an effect on their proliferation.

Key words: camellia seed cake, flavonoid, human dermal papilla cell, cell cycle, vascular endothelial growth factor

CLC Number:

TQ658

Shen Huchi,Chen Diansong,Yang Jingguo,Wang Jing. Study on the promotion of proliferation of dermal papilla cells and VEGF secretion by flavonoids from camellia seed cake[J].China Surfactant Detergent & Cosmetics, 2022, 52(1): 44-49.

Figures/Tables 5

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References 12

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样品	G0/G1/%	G2/M/%	S/%	PI/%
空白对照	58.3	3.34	20.7	29.2
10 μg/mL米诺地尔	51.8	7.75	22.5	36.9
20 μg/mL FE	49.2	7.61	26.0	40.6

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Study on the promotion of proliferation of dermal papilla cells and VEGF secretion by flavonoids from camellia seed cake

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