EGCG阳离子醇质体的制备及性能评价

doi:10.3969/j.issn.1001-1803.2021.02.003

Abstract

Abstract:

The cationic ethosomes were prepared by ethanol injection method which were added with the cationic surfactant dioctadecyl dimethyl ammonium chloride （TA-100）. The preparation conditions were optimized. The morphology, size distribution and Zeta potential of cationic ethosomes were determined by transmission electron microscopy and dynamic light scattering. Using epigallocatechin gallate （EGCG） as the hydrophilic model active substance, the encapsulation efficiency and in vitro release of EGCG loaded cationic ethosomes prepared with TA-100 were investigated, and their storage stability was also characterized. The results showed that the introduction of the cationic surfactant in ethosomes could not only effectively improve the stability of the ethosomes, but also significantly enhance the ability to encapsulate EGCG. The cationic ethosomes had good stability, high encapsulation capacity for active substances, and excellent slow release property, which showed good prospect for application in medicine, cosmetics and other fields.

Key words: dioctadecyl dimethyl ammonium chloride, cationic ethosome, epigallocatechin gallate

CLC Number:

TQ423

XU Pu,ZHANG Lei,SHANG Ya-zhuo,LIU Hong-lai. Preparation and performance evaluation of EGCG loaded cationic ethosomes[J].China Surfactant Detergent & Cosmetics, 2021, 51(2): 98-103.

Figures/Tables 8

Fig. 1

Tab. 1

Fig. 2

Tab. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

References 35

[1]	Pattni B S, Chupin V V, Torchilin V P. New developments in liposomal drug delivery[J]. Chemical Reviews, 2015,115(19) : 10938-10966. pmid: 26010257
[2]	Touitou E, Dayan N, Bergelson L, et al. Ethosomes-novel vesicular carriers for enhanced delivery: characterization and skin penetration properties[J]. Journal of Controlled Release, 2000,65(3) : 403-418.
[3]	Godin B, Touitou E. Ethosomes: new prospects in transdermal delivery[J]. Critical Reviews in Therapeutic Drug Carrier Systems, 2003,20(1) : 63. doi: 10.1615/critrevtherdrugcarriersyst.v20.i1.20 pmid: 12911264
[4]	Cosco D, Celia C, Cilurzo F, et al. Colloidal carriers for the enhanced delivery through the skin[J]. Expert Opinion on Drug Delivery, 2008,5(7) : 737. doi: 10.1517/17425247.5.7.737 pmid: 18590459
[5]	Zhang Y, Ng W, Feng X, et al. Lipid vesicular nanocarrier: quick encapsulation efficiency determination and transcutaneous application[J]. International Journal of Pharmaceutics, 2016,516(1/2) : 225-230.
[6]	Fang Y P, Tsai Y H, Wu P C, et al. Comparison of 5-aminolevulinic acid-encapsulated liposome versus ethosome for skin delivery for photodynamic therapy[J]. International Journal of Pharmaceutics, 2008,356(1) : 144-152.
[7]	Dayan N, Touitou E. Carriers for skin delivery of trihexyphenidyl HCl: ethosomes vs. liposomes[J]. Biomaterials, 2000,21(18) : 1879-1885.
[8]	Chen J, Yan G, Hu R R, et al. Improved pharmacokinetics and reduced toxicity of brucine after encapsulation into stealth liposomes: role of phosphatidylcholine[J]. International Journal of Nanomedicine, 2012: 3567-3577. pmid: 22904620
[9]	Wu L, Zhang J, Watanabe W. Physical and chemical stability of drug nanoparticles[J]. Advanced Drug Delivery Reviews, 2011,63(6) : 456-469. pmid: 21315781
[10]	Peng H, Li W, Ning F, et al. Amphiphilic chitosan derivatives-based liposomes: synjournal, development, and properties as a carrier for sustained release of salidroside[J]. Journal of Agricultural & Food Chemistry, 2013,62(3) : 626-633. doi: 10.1021/jf4039925 pmid: 24372377
[11]	Liang X F, Wang H J, Hao L, et al. Characterization of novel multifunctional cationic polymeric liposomes formed from octadecyl quaternized carboxymethyl chitosan/cholesterol and drug encapsulation[J]. Langmuir, 24(14) : 7147-7153. pmid: 18564860
[12]	Jain S, Kumar D, Swarnakar N K, et al. Polyelectrolyte stabilized multilayered liposomes for oral delivery of paclitaxel[J]. Biomaterials, 2012,33(28) : 6758-6768.
[13]	Chen L, Liang R, Wang Y, et al. Characterizations on the stability and release properties of β-ionone loaded thermosensitive liposomes (TSLs)[J]. Journal of Agricutural ＆ Food Chemistry, 2018,66(31) : 8336-8345.
[14]	Wu L, Zhang J, Watanabe W. Physical and chemical stability of drug nanoparticles[J]. Advanced Drug Delivery Reviews, 2011,63(6) : 456-469. doi: 10.1016/j.addr.2011.02.001 pmid: 21315781
[15]	Zhang Z, Wo Y, Zhang Y, et al. In vitro study of ethosome penetration in human skin and hypertrophic scar tissue[J]. Nanomedicine Nanotechnology Biology & Medicine, 2012,8(6) : 1026-1033.
[16]	Malheiros P D S, Micheletto Y M S, Silveira N P D, et al. Development and characterization of phosphatidylcholine nanovesicles containing the antimicrobial peptide nisin[J]. Food Research International, 2010,43(4) : 1198-1203.
[17]	Sułkowski W W, Pentak D, Nowak K, et al. The influence of temperature, cholesterol content and pH on liposome stability[J]. Journal of Molecular Structure, 2005,744-747:737-747.
[18]	Lu Q, Li D C, Jiang G J. Preparation of a tea polyphenol nanoliposome system and its physicochemical properties[J]. Journal of Agricultural & Food Chemistry, 2011,59(24) : 13004. pmid: 22087534
[19]	Zhuang Y H, Zhang Z W, Han W, et al. Determination of entrapment efficiency of forsythosideliposome by ultrafiltration[J]. Chinese Journal of New Drugs, 2012,21(18) : 2209-2211, 2216.
[20]	Xing C, Li Q Z, Jing N, et al. The stability, sustained release and cellular antioxidant activity of curcumin nanoliposomes[J]. Molecules, 2015,20(8) : 14293-14311. doi: 10.3390/molecules200814293 pmid: 26251892
[21]	Zou L Q, Liu W, Liu W L, et al. Characterization and bioavailability of tea polyphenol nanoliposome prepared by combining an ethanol injection method with dynamic high-pressure microfluidization[J]. Journal of Agricultural & Food Chemistry, 2014,62(4) : 934-941. pmid: 24428744
[22]	Sun Hongsheng. Preparation and characterization of berberine hydrochloride and trimethoprim[D]. Chongqing: Chongqing University, 2017.
[23]	Zhang Chunyan, Xu Xiaoming, Wu Zhangfeng, et al. Investigation on the interaction between quaternary ammonium surfactants and lecithin vesicles[J]. Acta Chimica Sinica, 2005,63(6) : 450-454.
[24]	Elsayed M M, Abdallah O Y, Naggar V F, et al. Deformable liposomes and ethosomes: mechanism of enhanced skin delivery[J]. International Journal of Pharmaceutics, 2006,322(1) : 60-66.
[25]	Yin J, Becker E M, Andersen M L, et al. Green tea extract as food antioxidant. Synergism and antagonism with α-tocopherol in vegetable oils and their colloidal systems[J]. Food Chemistry, 2012,135(4) : 2195-2202. doi: 10.1016/j.foodchem.2012.07.025 pmid: 22980790
[26]	Holmstrm S C, Patil A J, Butler M, et al. Influence of polymer co-intercalation on guest release from aminopropyl-functionalized magnesium phyllosilicate mesolamellar nanocomposites[J]. Journal of Materials Chemistry, 2007,17(37) : 3894-3900.
[27]	Huang J, Liu Y, Wang X. Selective Adsorption of tannin from flavonoids by organically modified attapulgite clay[J]. Journal of Hazardous Materials, 2008,160(2/3):382-387.
[28]	Li Y M, Jiang T, Lv Yin, et al. Amphiphilic copolymers with pendent carboxyl groups for high-efficiency loading and controlled release of doxorubicin[J]. Colloids & Surfaces B: Biointerfaces, 2015,132:54-61. pmid: 26005931
[29]	Lee D H, Jin B H, Hwang Y, et al. Encapsulation of bromelain in liposome[J]. Preventive Nutrition & Food Science, 2000,5(2) : 81-85.
[30]	Ramadon D, Pramesti S S, Anwar E. Formulation, stability test, and in vitro penetration study of transethosomal gel containing green tea (Camellia sinensis L. Kuntze) leaves extract[J]. International Journal of Applied Pharmaceutics, 2017,9(5) : 91-96.
[31]	Zuo Xiaobo, Kong Junhao, Sun Xiaoqin, et al. Preparation of EGCG multilayer emulsion based on the self-assembly technology and its microencapsulation[J]. Science and Technology of Food Industry , 2019,40(9) : 38-43.
[32]	Huang Weichao, Liu Chunhua, Luo Xuguang, et al. Fabrication and formation mechanism of EGCG–loaded nanoparticles based on the self: assembly of sodium caseinate, EGCG and gum arabic[J]. Science and Technology of Food Industry, 2019,40(10) : 93-101, 108.
[33]	Zhang W H, Yang Y Y, Lv T, et al. Sucrose esters improve the colloidal stability of nanoethosomal suspensions of (-)-epigallocatechin gallate for enhancing the effectiveness against UVB-induced skin damage[J]. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2017,105(8) : 2416-2425. doi: 10.1002/jbm.b.33785 pmid: 27618624
[34]	Liang J, Cao L, Zhang L, Yu C H, et al. Preparation, characterization, and in vitro antitumor activity of folate conjugated chitosan coated EGCG nanoparticles[J]. Food Science and Biotechnology, 2014,23(2) : 569-575.
[35]	Hsieh D S, Lu H C, Chen C C, et al. The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system[J]. International Journal of Nanomedicine, 2012,7:1623-1633. doi: 10.2147/IJN.S30060 pmid: 22615529

TA-100 质量分数/%	d/nm	PDI	Zeta电位/mV
0	350.8 ±3.0	0.617±0.028	1.70±0.10
0.01	97.12±0.32	0.184±0.012	36.9 ±3.9
0.02	93.90±0.84	0.222±0.048	42.6 ±1.2
0.03	90.71±1.13	0.252±0.001	45.9 ±1.2
0.04	108.7 ±2.6	0.348±0.015	43.3 ±0.6
0.05	111.7 ±0.4	0.384±0.019	30.8 ±0.3

样品	d/nm	PDI	Zeta电位/mV	包封率/%
EGCG阳离子醇质体	129.3±1.9	0.224±0.004	39.9 ±0.9	68.09±2.92
EGCG醇质体	434.5±4.2	0.644±0.030	2.10±0.10	35.20±1.30

Preparation and performance evaluation of EGCG loaded cationic ethosomes

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 35

Related Articles 2

Metrics

Comments

Recommended 10

[1]	CAO Zhen-da,LIANG Rong. Study on the preparation and transdermal performance of epigallocatechin gallate liposome [J]. China Surfactant Detergent & Cosmetics, 2020, 50(9): 609-614.
[2]	DENG Xiao-feng, WANG Hao, ZHOU Zi-ruo, MENG Hong, LI Li. Mechanisms of efficacies of epigallocatechin gallate in cosmetics [J]. China Surfactant Detergent & Cosmetics, 2015, 45(10): 589-592.