灯盏花化妆品原料的抗氧化活性及质量控制研究

doi:10.3969/j.issn.1001-1803.2021.12.006

Abstract

Abstract:

Erigeron breviscapus （vant.） Hand. Mazz. is one of the most important traditional Chinese medicinal plants for cardiopathy. Erigeron breviscapus mainly contains flavonoids and caffeic acids, which have obvious antioxidant activities. To study the possibility of developing Erigeron breviscapus as a cosmetics material, the antioxidant activity of Erigeron breviscapus cosmetic ingredients （EBCI） was studied and a method for simultaneous determination of two index compounds therein was established. The scavenging activities of EBCI against DPPH, ABTS and ·OH radicals were evaluated by in vitro method. The effects of EBCI on cell viability and glutathione （GSH） content were investigated by using H₂O₂-induced damage in HHF-1 cells. HPLC method for simultaneous determination of scutellarin and 4,5-dicaffeoylquinic acid in EBCI was established. The results show that EBCI have scavenging activities against DPPH, ABTS and ·OH radicals, with IC₅₀ values being 46.93, 91.36 and 89.37 μg/mL, respectively. The cell viability of H₂O₂-induced injury in HFF-1 cells and the GSH content in the cells could be significantly increased at the concentrations of 4 and 20 μg/mL. 4,5-Dicaffeoylquinic acid and scutellarin showed good linearity in the linear ranges of 0.008 5-0.340 9 mg/mL and 0.009 3-0.372 2 mg/mL, respectively. Average recoveries of scutellarin and 4,5-dicaffeoylquinic acid were 103.47% and 103.38% （RSD<2%）, respectively. This method is accurate, simple and well reproducible, and can be used for the quality control of EBCI. It shows the antioxidant activity of EBCI in this study.

Key words: Erigeron breviscapus, cosmetic ingredient, antioxidant, scutellarin, 4,5-dicaffeoylquinic acid, content determination

CLC Number:

TQ658

Li Qian,Xu Ming,Xiao Ruyue,Luo Chun,Li Zhanguo,Mei Shuangxi. Antioxidant activity analysis and quality control of cosmetic ingredients from Erigeron breviscapus[J].China Surfactant Detergent & Cosmetics, 2021, 51(12): 1202-1207.

Figures/Tables 6

Tab. 1

Tab. 2

Tab. 3

Fig. 1

Tab. 4

Tab. 5

References 10

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时间/min	φ（流动相A）/%	φ（流动相B）/%
0~10	95→88	5→12
10~15	95→85	12→15
15~30	85→84	15→16
30~40	84	16
40~55	84→83	16→17
55~60	83→10	17→90

样品	DPPH		ABTS		·OH
样品	ρ/（μg·mL^-1）	清除率/%	ρ/（μg·mL^-1）	清除率/%	ρ/（μg·mL^-1）	清除率/%
EBCI	125	87.91±0.28	250	91.90±1.22	250	85.23±2.69
	62.5	62.82±7.10	125	65.36±0.94	125	60.93±5.59
	31.25	31.79±0.16	62.5	31.41±0.28	62.5	38.36±4.91
	15.625	17.10±1.19	31.25	18.41±0.19	31.25	24.35±1.66
Vc	20.0	93.43±1.46	20.0	83.81±1.98	125	81.81±3.78
	10.0	66.96±12.83	10.0	35.12±2.85	62.5	53.95±3.21
	5.0	35.57±4.08	5.0	20.54±2.09	31.25	37.41±0.57
	2.5	19.92±3.78	2.5	14.17±3.58	15.625	28.86±5.97

组别	ρ/（μg·mL^-1）	细胞存活率/%	GSH含量/（μmol·gprot^-1）
空白对照组	—	100.00±1.08	20.73±4.21
模型组	—	35.79±2.41^###	13.53±1.60^#
EBCI	100	41.24±2.85	19.54±1.51^*
	20	42.43±3.13^*	20.47±3.15^*
	4	41.51±1.21^*	23.63±3.42^*
V_E	100	92.63±11.56^***	20.52±3.61^*
	20	50.67±7.07^*	19.85±2.24^*
	4	34.88±4.11	—

对照品	称样量/mg	样品中含量/mg	加入量/mg	测得量/mg	回收率/%	平均值/%	RSD值/%
野黄芩苷	10.4	0.359 5	0.348 9	0.719 2	103.08	103.47	0.58
	10.2	0.352 6	0.348 9	0.713 8	103.52
	10.7	0.369 9	0.348 9	0.733 3	104.15
	10.6	0.366 5	0.348 9	0.724 4	102.58
	10.3	0.356 1	0.348 9	0.719 2	104.08
	10.5	0.363 0	0.348 9	0.723 9	103.43
4,5-二-O-咖啡酰奎宁酸	10.0	0.145 4	0.127 8	0.280 0	105.30	103.38	1.58
	10.3	0.136 9	0.127 8	0.267 3	102.04
	10.7	0.141 7	0.127 8	0.271 8	101.77
	10.4	0.126 0	0.127 8	0.256 4	102.00
	10.1	0.122 3	0.127 8	0.255 5	104.16
	10.7	0.129 6	0.127 8	0.263 9	105.04

样品批号	w（野黄芩苷）/ %	w（4,5-二-O-咖啡酰奎宁酸）/ %
EBCI-1	3.52	1.26
EBCI-2	3.65	1.40
EBCI-3	3.36	1.40
EBCI-4	3.23	1.00
EBCI-5	3.72	1.11

Antioxidant activity analysis and quality control of cosmetic ingredients from Erigeron breviscapus

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