3种化合物在UV诱导下对CHL细胞染色体畸变作用

doi:10.3969/j.issn.1001-1803.2019.06.005

Abstract

Abstract:

The effects of CPZ, acid red 87 and alizarin red on chromosome aberration in CHL cells induced by UV were studied. The cytotoxicity test and chromosome aberration test of CHL cells exposed to the above three compounds were carried out at a UV dose of 2.0 J/cm ². Chromosome aberration rate of CHL cells caused by these compounds were observed and analyzed. The results show that in vitro chromosome aberration test system of CHL cells is set up, and the three compounds had no chromosome aberration effect in the absence of UV. Under UV stimulation, CPZ causes chromosome aberration in CHL cells at a mass concentration range of 0.25-0.50 mg/L（without S₉ mix）. Acidic red 87 induces chromosome aberration in CHL cells in the range of 15.62-31.25 mg/L（without S₉ mix）and 31.25-62.50 mg/L（with S₉ mix）. Alizarin red can not induce chromosome aberration in CHL cells.

Key words: cosmetic material, UV dose, chromosome aberration, chlorpromazine hydrochloride, acid red 87, alizarin red

CLC Number:

TQ658

LI Pei-ning,CHEN Xiu-juan,JIANG Yi,LIU Dong-hong. Effects of three compounds on chromosome aberration in CHL cells induced by UV[J].China Surfactant Detergent & Cosmetics, 2019, 49(6): 377-380.

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References 8

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Effects of three compounds on chromosome aberration in CHL cells induced by UV

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受试物	ρ/（mg·L^-1）	S₉mix	中期分裂相/个	min	t	b	裂隙	畸变率/%
DPBS	0	+	100	0	0	2	1	2
DPBS	0	-	100	1	0	1	0	2
CP	7.5	+	100	1	23	10	3	32^**
MMC	0.4	-	100	11	2	5	1	18^**
CPZ	1.56	-	100	2	1	0	1	3
	3.12	+	100	1	0	0	0	1
	3.12	-	100	2	0	1	1	2
	6.25	+	100	2	0	0	1	2
	6.25	-	100	0	1	2	1	2
	12.50	+	100	0	0	2	0	2
酸性红87	7.81	-	200	4	0	3	0	3
	15.62	+	200	0	2	0	0	1
	15.62	-	200	2	0	2	0	2
	31.25	+	200	3	0	5	0	4
	31.25	-	200	0	0	4	2	2
	62.50	+	200	4	2	11	2	7
茜素红	250	+	200	0	0	4	0	2
	250	-	200	2	0	5	0	3
	500	+	200	0	0	8	0	4
	500	-	200	1	0	7	0	4
	1 000	+	200	3	0	2	0	2
	1 000	-	200	2	0	0	2	1

受试物	ρ/（mg·L^-1）	S₉mix	中期分裂相/个	min	t	b	r	粉碎	裂隙	畸变率/%
DPBS	0	+	100	0	0	2	0	0	1	2
DPBS	0	-	100	1	0	1	0	0	0	2
CPZ	0.12	+	100	3	0	1	0	0	2	4
	0.12	-	100	6	0	2	0	0	1	7
	0.25	+	100	0	0	7	0	0	1	7
	0.25	-	100	6	3	3	0	1	1	12^**
	0.50	+	100	2	1	1	0	0	0	4
	0.50	-	100	5	4	7	1	1	1	17^**
酸性红87	7.81	-	200	4	0	11	0	0	2	7
	15.62	+	200	8	2	8	0	0	2	8
	15.62	-	200	4	0	14	0	0	0	9^*
	31.25	+	200	6	0	18	0	0	3	11^*
	31.25	-	200	7	2	10	1	0	2	11^*
	62.50	+	200	7	0	17	0	0	0	12^**
茜素红	250	+	200	0	0	4	0	0	0	2
	250	-	200	4	0	2	0	0	0	3
	500	+	200	1	0	5	0	0	0	3
	500	-	200	2	0	2	0	0	0	2
	1 000	+	200	4	0	13	0	0	0	8
	1 000	-	200	4	0	2	0	0	0	3

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