Abstract: Human dermal lymphatic capillaries were reported to have impact on skin homeostasis in recent researches.Human dermal lymphatic endothelial cells (HDLEC) were extracted from normal human skin biopsy and enriched with a two-step magnetic microbeads-based separation method.The proportion of Podoplanin-positive cells in HDLEC culture was evaluated by flow cytometry as 95.7%.To evaluate the skin care efficacy of Himalaya glacier water,an in vitro lymphatic capillary permeability test model was reconstructed based on HDLEC culture,and the test result shows the relative permeability of HDLEC monolayer turned to 49.3%±17.0% (P<0.05) after the treatment of glacier water compared with that from the untreated control.In the meantime,immuno-cytochemistry staining targeting endothelial cell-cell junction markers show relative expressions of VE-Cadherin and ZO-1 in glacier water-treated HDLEC are significantly up-regulated than the untreated control(P<0.05).Therefore,Himalaya glacier water is proved to be active in reinforcing the stability of dermal lymphatic capillaries to maintain skin homeostasis.

Key words: skin protection, dermis, lymphatic endothelial cell, immuno-magnetic separation, capillary permeability