侧柏枝挥发油促进雄激素性脱发小鼠毛发生长研究

doi:10.3969/j.issn.2097-2806.2026.05.009

Abstract

Abstract:

Supercritical CO₂ extraction was employed to isolate the essential oil from Platycladus orientalis L. branches （EOPB）. The extraction process was optimized using a Box-Behnken design （BBD） with the extraction yield as the response variable. The chemical constituents of the extracted essential oil were characterized via gas chromatography-mass spectrometry. An androgenetic alopecia （AGA） model was established in C57BL/6 mice by depilating the dorsal hair and administering daily intraperitoneal injections of testosterone to evaluate the hair growth-promoting effects of EOPB. The underlying mechanisms were investigated using network pharmacology. Furthermore, the cutaneous levels of IL-1β, IL-6, TNF-α, SOD, CAT, and MDA were measured using ELISA kits. The results indicate that the optimal extraction parameters for EOPB are a pressure of 10.62 MPa, a temperature of 43.69 ℃, and an extraction time of 83.86 minutes, achieving a maximum extraction yield of 20.107%. The primary constituents of EOPB are identified as thujopsene （32.27%） and cedrol （20.88%）. The 10% （φ/%） EOPB group achieves complete hair regrowth by day 21, exhibiting an efficacy comparable to that of the minoxidil positive control. Histological analysis confirms that EOPB enhances hair follicle density and accelerates the anagen phase transition. Network pharmacology analysis reveals the involvement of EOPB in inflammatory and oxidative stress pathways. EOPB effectively suppresses inflammatory factors （IL-1β, IL-6, TNF-α）, restores antioxidant enzyme （SOD and CAT） activities, and reduces lipid peroxidation （MDA levels）. This study provides a reference for the application of EOPB in hair care products, offering alternative therapeutic choices for patients with androgenetic alopecia.

Key words: Platycladus orientalis L. branch essential oil, androgenetic alopecia, supercritical fluid extraction, GC-MS, network pharmacology

CLC Number:

TQ658

Lei Zhang, Zihan He, Jun Yang, Xiaowei Zhou, Zhenhua Wang, Gang Li, Chengjun Ma. Study on essential oil from Platycladus orientalis L. branches for promoting hair growth in androgenetic alopecia mice[J].China Surfactant Detergent & Cosmetics, 2026, 56(5): 623-634.

Figures/Tables 11

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References 30

[1]	Natarelli N, Gahoonia N, Sivamani R K. Integrative and mechanistic approach to the hair growth cycle and hair loss[J]. Journal of Clinical Medicine, 2023, 12 (3) : 893.
[2]	Wang Wuji, Wang Honglan, Long Yunluan, et al. Controlling hair loss by regulating apoptosis in hair follicles: a comprehensive overview[J]. Biomolecules, 2024, 14 (1) : 20.
[3]	Devjani S, Ezemma O, Kelley K J, et al. Androgenetic alopecia: Therapy update[J]. Drugs, 2023, 83 (8) : 701-715. doi: 10.1007/s40265-023-01880-x pmid: 37166619
[4]	Katzer T, Leite Junior A, Beck R, et al. Physiopathology and current treatments of androgenetic alopecia: Going beyond androgens and anti-androgens[J]. Dermatologic Therapy, 2019, 32 (5) : e13059.
[5]	Williams R, Pawlus A D, Thornton M J. Getting under the skin of hair aging: The impact of the hair follicle environment[J]. Experimental Dermatology, 2020, 29 (7) : 588-597. doi: 10.1111/exd.v29.7
[6]	Cai Yizuo, Jia Zhuoxuan, Zhang Yichen, et al. Cell-free fat extract restores hair loss: A novel therapeutic strategy for androgenetic alopecia[J]. Stem Cell Research & Therapy, 2023, 14 (1) : 219.
[7]	Olsen E A, Whiting D, Bergfeld W, et al. A multicenter, randomized, placebo-controlled, double-blind clinical trial of a novel formulation of 5% minoxidil topical foam versus placebo in the treatment of androgenetic alopecia in men[J]. Journal of the American Academy of Dermatology, 2007, 57 (5) : 767-774. doi: 10.1016/j.jaad.2007.04.012 pmid: 17761356
[8]	Hosking A M, Juhasz M, Mesinkovska N A. Complementary and alternative treatments for alopecia: A comprehensive review[J]. Skin Appendage Disorders, 2018, 5 (2) : 72-89. doi: 10.1159/000492035
[9]	Tosti A, Piraccini B M. Finasteride and the hair cycle[J]. Journal of the American Academy of Dermatology, 2000, 42 (5) : 848-849.
[10]	Li Guoqing, Du Sheng, Wen Zhongming. Mapping the climatic suitable habitat of oriental arborvitae (Platycladus orientalis) for introduction and cultivation at a global scale[J]. Scientific Reports, 2016, 6 (1) : 30009.
[11]	Zhang N N, Park D K, Park H J. Hair growth-promoting activity of hot water extract of Thuja orientalis[J]. BMC Complementary and Alternative Medicine, 2013, 13 (1) : 9.
[12]	Adil A, Godwin M. The effectiveness of treatments for androgenetic alopecia: A systematic review and meta-analysis[J]. Journal of the American Academy of Dermatology, 2017, 77 (1) : 136-141. doi: S0190-9622(17)30306-7 pmid: 28396101
[13]	Nestor M S, Ablon G, Gade A, et al. Treatment options for androgenetic alopecia: Efficacy, side effects, compliance, financial considerations, and ethics[J]. Journal of Cosmetic Dermatology, 2021, 20 (12) : 3759-3781. doi: 10.1111/jocd.14537 pmid: 34741573
[14]	Zhang Yan, Han Ling, Chen Shanshan, et al. Hair growth promoting activity of cedrol isolated from the leaves of Platycladus orientalis[J]. Biomedicine & Pharmacotherapy, 2016, 83: 641-647. doi: 10.1016/j.biopha.2016.07.022
[15]	Bakkali F, Averbeck S, Averbeck D, et al. Biological effects of essential oils: A review[J]. Food and Chemical Toxicology, 2008, 46(2) : 446-475. doi: 10.1016/j.fct.2007.09.106 pmid: 17996351
[16]	Chandharakool S, Koomhin P, Sinlapasorn J, et al. Effects of tangerine essential oil on brain waves, moods, and sleep onset latency[J]. Molecules, 2020, 25 (20) : 4865.
[17]	Wang Xiangzeng, Sun Xiao, Guo Xing, et al. Study on the influence of supercritical CO₂ with high temperature and pressure on pore-throat structure and minerals of shale[J]. ACS Omega, 2024, 9 (13) : 15259-15270. doi: 10.1021/acsomega.3c09698 pmid: 38585050
[18]	Tsiaka T, Sinanoglou V J, Zoumpoulakis P. Chapter 8-extracting bioactive compounds from natural sources using green high-energy approaches: Trends and opportunities in lab- and large-scale applications[M]. New York: Academic Press, 2017: 307-365.
[19]	Patil P D, Gude V G, Mannarswamy A, et al. Optimization of direct conversion of wet algae to biodiesel under supercritical methanol conditions[J]. Bioresource Technology, 2011, 102 (1) : 118-122. doi: 10.1016/j.biortech.2010.06.031 pmid: 20591655
[20]	Liu Zenggen, Mei Lijuan, Wang Qilan, et al. Optimization of subcritical fluid extraction of seed oil from Nitraria tangutorum using response surface methodology[J]. LWT-Food Science and Technology, 2014, 56 (1) : 168-174. doi: 10.1016/j.lwt.2013.10.048
[21]	Olsen E A, Whiting D, Bergfeld W, et al. A multicenter, randomized, placebo-controlled, double-blind clinical trial of a novel formulation of 5% minoxidil topical foam versus placebo in the treatment of androgenetic alopecia in men[J]. Journal of the American Academy of Dermatology, 2007, 57 (5) : 767-774. doi: 10.1016/j.jaad.2007.04.012 pmid: 17761356
[22]	Hoch C C, Petry J, Griesbaum L, et al. 1, 8-cineole (eucalyptol) : A versatile phytochemical with therapeutic applications across multiple diseases[J]. Biomedicine & Pharmacotherapy, 2023, 167: 115467. doi: 10.1016/j.biopha.2023.115467
[23]	Ding Linlin, Xu Lei, Hu Na, et al. Deciphering the therapeutic potential and mechanisms of Artemisia argyit essential oil on flagellum-mediated Salmonella infections[J]. Chinese Journal of Natural Medicines, 2025, 23 (6) : 714-726. doi: 10.1016/S1875-5364(25)60890-9
[24]	Gheisari M, Hamidi A B, Hamedani B, et al. Androgenetic alopecia; An attempt to target microinflammation[J]. Dermatologic Therapy, 2020, 33 (2) : e13266.
[25]	Ramos P M, Brianezi G, Martins A C, et al. Apoptosis in follicles of individuals with female pattern hair loss is associated with perifollicular microinflammation[J]. International Journal of Cosmetic Science, 2016, 38 (6) : 651-654. doi: 10.1111/ics.12341 pmid: 27163333
[26]	Halliwell B. Understanding mechanisms of antioxidant action in health and disease[J]. Nature Reviews Molecular Cell Biology, 2024, 25(1) : 13-33. doi: 10.1038/s41580-023-00645-4
[27]	Roleira F M, Tavares-da-Silva E J, Varela C L, et al. Plant derived and dietary phenolic antioxidants: Anticancer properties[J]. Food Chemistry, 2015, 183: 235-258. doi: 10.1016/j.foodchem.2015.03.039 pmid: 25863633
[28]	Naziroglu M, Kokcam I. Antioxidants and lipid peroxidation status in the blood of patients with alopecia[J]. Cell Biochemistry and Function, 2000, 18 (3) : 169-173. pmid: 10965354
[29]	Kops G J, Dansen T B, Polderman P E, et al. Forkhead transcription factor FOXO3a protects quiescent cells from oxidative stress[J]. Nature, 2002, 419 (6904) : 316-321. doi: 10.1038/nature01036
[30]	Qin Jianzhong, Xin Hong, Qi Xiaomei, et al. Isoform-specific and cell/tissue-dependent effects of p38 MAPKs in regulating inflammation and inflammation-associated oncogenesis[J]. Frontiers in Bioscience Landmark, 2022, 27 (1) : 31.

Source	Sum of squares	df	Mean square	F-value	P-value	Significance
Model	238.53	9	26.50	26.31	0.000 1	***
A: Pressure	22.92	1	22.92	22.75	0.002	**
B: Temperature	0.973 0	1	0.973 0	0.966 0	0.358 4	NS
C: Time	0.931 6	1	0.931 6	0.924 9	0.368 2	NS
AB	0.616 2	1	0.616 2	0.611 8	0.459 8	NS
AC	19.23	1	19.23	19.09	0.003 3	**
BC	7.02	1	7.02	6.97	0.033 4	*
A²	11.48	1	11.48	11.40	0.011 8	*
B²	144.05	1	144.05	143.01	<0.000 1	***
C²	18.37	1	18.37	18.24	0.003 7	**
Residual	7.05	7	1.01	N/A	N/A	N/A
Lack of fit	2.42	3	0.808 1	0.698 7	0.599 9	NS
Pure error	4.63	4	1.16	N/A	N/A	N/A
Cor total	245.58	16	N/A	N/A	N/A	N/A
R²=0.971 3	Adjusted R²=0.934 4		Predicted R²=0.812 6		Adeq precision=12.674 7

	Count/ %	Retention time/min	Chemical formula	Compound
1	32.27	52.695	C₁₅H₂₄	Thujopsene
2	2.66	56.134	C₁₅H₂₂	Cuparene
3	3.26	56.367	C₁₅H₂₄	β-Cedrene
4	1.62	57.551	C₁₅H₂₂	9, 10-Dehydroisonofolene
5	3.16	60.316	C₁₅H₂₆O	α-Bisabolol
6	20.88	60.922	C₁₅H₂₆O	Cedrol
7	4.43	61.022	C₁₀H₁₈O	β-Eucalyptol
8	1.73	61.63	C₁₅H₂₄	Rohanberol
9	1.87	62.274	C₁₅H₂₄	α-Longipinene
10	1.84	62.818	C₁₅H₂₂	α-Curcumone
11	1.52	64.054	C₁₅H₂₄	Isolongifolene
12	2.55	64.403	C₁₅H₂₄	α-Cedrene
13	2.6	65.298	C₁₅H₂₄	γ-Muronene

Study on essential oil from Platycladus orientalis L. branches for promoting hair growth in androgenetic alopecia mice

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	Pressure/MPa（A）	Temperature/℃（B）	Time/min（C）	Actual yield/%	Predicted yield/%	Residual/%
1	20	45	90	18.33	19.19	-0.86
2	10	35	90	14.48	14.12	0.36
3	30	45	100	15.28	15.61	-0.33
4	20	45	90	18.9	19.19	-0.29
5	20	55	100	12.57	11.89	0.68
6	20	35	100	9.62	9.93	-0.31
7	10	45	100	14.56	14.61	-0.05
8	20	45	90	19.93	19.19	0.74
9	20	45	90	20.65	19.19	1.46
10	20	45	90	18.13	19.19	-1.06
11	20	35	80	12.58	13.27	-0.69
12	10	45	80	20.03	19.27	0.76
13	20	55	80	10.23	9.92	0.31
14	30	45	80	11.59	11.9	-0.31
15	10	55	90	12	12.64	-0.64
16	30	55	90	9.68	10.04	-0.36
17	30	35	90	10.59	9.95	0.64

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