Abstract:

The aim of this study was to develop a method for the quantification of adenosine and cordycepin in Cordyceps sinensis cosmetics using ultra performance liquid chromatography coupled with tandem mass spectrometry （UPLC-MS/MS）. This method is applicable to various cosmetic matrices, including creams, lotions, and both water-based and oil-based liquids, and was employed to detect and analyze commercially available products. Samples were subjected to ultrasonic extraction using 80% methanol, followed by gradient elution with a water-acetonitrile solution （containing 0.05% （V/V） formic acid）. Rapid separation was achieved using a C₁₈ ultra performance liquid chromatography column, and detection was performed in positive ion multiple reaction monitoring （MRM） mode. The detection limits for adenosine and cordycepin were set at 0.000 4 mg/kg and 0.002 5 mg/kg, respectively. Within the mass concentration range of 1 to 20 ng/mL, both compounds demonstrate a strong linear relationship. At low mass concentrations, the recovery rates range from 86.8% to 108.0%, with an RSD of ≤4.8% （n=6）. For medium and high mass concentrations, the recovery rates are between 96.6% and 112.2%, with an RSD of ≤6.9% （n=6）. Standard solutions and spiked solutions remain stable for up to 72 hours when store at 4 ℃ in the dark. Furthermore, adenosine is detected in three batches of commercial cosmetics, while cordycepin is not detected in any of the tested batches. The developed method exhibits high detection efficiency and sensitivity, as well as excellent accuracy and precision, making it a valuable auxiliary regulatory tool for verifying the claims of Cordyceps sinensis cosmetic ingredient additions. The accumulated data can provide robust technical support for the development and revision of raw material standards.

Key words: Cordyceps sinensis, cosmetics, adenosine, cordycepin, ultra performance liquid chromatography coupled with tandem mass spectrometry, market regulation