重组人源化Ⅲ型胶原蛋白对皮肤功能性相关基因 表达的影响

doi:10.3969/j.issn.1001-1803.2022.12.010

摘要/Abstract

摘要：

重组人源化Ⅲ型胶原蛋白（RHC Ⅲ）由人胶原原始基因序列经过优化重组表达所得，与人体天然胶原氨基酸序列高度一致。为探究重组人源化Ⅲ型胶原蛋白应用在化妆品中，对减缓皮肤皱纹的功效作用，通过测试其作用于人皮肤成纤维细胞和角质形成细胞，在皮肤功能性基因层面验证其抗皱功效。结果表明，5 mg/mL RHC Ⅲ作用于人皮肤成纤维细胞可显著促进胶原合成相关基因Collagen Ⅰ，Collagen Ⅲ和Smad3的表达；同时，在UVA刺激条件下可显著抑制胶原降解相关基因MMP-1，MMP-3，c-jun和c-fos的表达。作用于角质形成细胞可显著促进皮肤屏障修复和保湿相关基因LOR，TGM1，IVL，OCLN1，DSG1，FLG，Caspase14和K1的表达。综上所述，样品重组人源化Ⅲ型胶原蛋白在 5 mg/mL的给药质量浓度下具有抗皱功效。

关键词: 重组人源化Ⅲ型胶原蛋白, 胶原合成和降解, 屏障修复和保湿, 抗皱

Abstract:

At present, the extraction of collagen is mainly from animal skin tissues, such as pigs, cattle, fish, and so on, which has certain virus hidden trouble. In order to obtain safer and better collagen, scholars have used gene recombination technology to produce recombinant human collagen. Type Ⅲ collagen is the main collagen in human skin, so the use of recombinant type Ⅲ collagen is of great significance for the study of skin properties. The recombinant humanized type Ⅲ collagen is obtained by optimizing recombinant expression of a human collagen original gene sequence that is highly consistent with a human natural collagen amino acid sequence. In order to explore the effect of recombinant humanized type Ⅲ collagen on alleviating skin wrinkles when applied to cosmetics, its anti-wrinkle effect was verified at the skin functional gene level by testing on human skin fibroblasts and keratinocytes. The results show that 5 mg/mL RHC Ⅲ can significantly promote the expression of collagen synthesis-related genes Collagen Ⅰ, Collagen Ⅲ and Smad3 in human skin fibroblasts. Meanwhile, under UVA stimulation, the expression of collagen degradation related genes MMP-1, MMP-3, c-jun and c-fos can be significantly inhibited. Acting on keratinocytes can significantly promote the expression of skin barrier and moisturizing-related genes LOR, TGM1, IVL, OCLN1, DSG1, FLG, Caspase14 and K1.To sum up, the sample recombinant type Ⅲ collagen has anti-wrinkle effect at the mass concentration of 5 mg/mL.

Key words: recombinant humanized type Ⅲ collagen, collagen synthesis and degradation, barrier repair and moisturizing, anti-wrinkle

中图分类号:

TQ658

范婷,赵健烽,常烨珺,季乐. 重组人源化Ⅲ型胶原蛋白对皮肤功能性相关基因表达的影响[J]. 日用化学工业（中英文）, 2022, 52(12): 1326-1332.

Fan Ting,Zhao Jianfeng,Chang Yejun,Ji Le. Effect of recombinant humanized type Ⅲ collagen on expression of skin functional related genes[J]. China Surfactant Detergent & Cosmetics, 2022, 52(12): 1326-1332.

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