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日用化学工业 ›› 2021, Vol. 51 ›› Issue (10): 925-931.doi: 10.3969/j.issn.1001-1803.2021.10.001

• 基础研究 •    下一篇

液滴模板法构建疏水杂化囊用于包封SOD酶研究

邓宁君,唐启明,洪美莹,孙梦梦,郭婷,孟涛()   

  1. 西南交通大学 生命科学与工程学院,四川 成都 610031
  • 收稿日期:2020-12-24 修回日期:2021-09-08 出版日期:2021-10-22 发布日期:2021-10-21
  • 通讯作者: 孟涛
  • 基金资助:
    国家自然科学基金资助项目(21776230);四川省科技厅重点研发项目(2020YFG0092)

Preparation of the hydrophobic hybrid capsule via droplet template method for encapsulating SOD

Deng Ningjun,Tang Qiming,Hong Meiying,Sun Mengmeng,Guo Ting,Meng Tao()   

  1. School of Life Sciences & Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031, China
  • Received:2020-12-24 Revised:2021-09-08 Online:2021-10-22 Published:2021-10-21
  • Contact: Tao Meng

摘要:

针对化妆品中生物活性分子保存稳定性问题,通过液滴模板法制备包封超氧化物歧化酶(SOD)的碳酸钙@海藻酸钙(CaCO3@Ca-Alg)杂化囊,再疏水改性壁层来阻止水分子进入,从而提高了包封物的保存稳定性。实验结果表明:该包封SOD的疏水杂化囊具有粒径均一、单分散性良好的优点;随着改性时间的延长,杂化囊疏水性随之提高,当改性时间超过4 h后,杂化囊疏水性保持稳定;杂化囊固定SOD的相对酶活明显高于单纯Ca-Alg囊(最大差值达到了300%);游离SOD在去离子水中保存4 d后完全失活,而包封SOD的疏水杂化囊保存7 d后仍能维持95%以上的相对酶活。

关键词: 液滴模板法, 杂化囊, 固定化酶, 疏水性

Abstract:

To solve the problems in the storage stability of bioactive molecules in cosmetics, the CaCO3@Ca-Alg hybrid capsule with encapsulated superoxide dismutase (SOD) was prepared by droplet template method. Combined with the hydrophobic modification of the shell, the hydrophobic layer could prevent the infiltration of water molecules, thus improving the storage stability of the encapsulated substance. The results show that the SOD loaded hydrophobic hybrid capsule possesses uniform particle size and good monodispersity. The hydrophobicity of the hybrid capsule is improved with the increase of modification time. When the modification time is over 4 h, the hydrophobicity of the hybrid capsule remains stable. The relative enzyme activity of SOD encapsulated by this hybrid capsule is significantly higher than that of simple Ca-Alg capsule (the maximum difference can reach 300%). The hydrophobic hybrid capsule can significantly improve the storage stability of SOD which will maintain more than 95% of the relative activity within 7 days.

Key words: droplet template method, hybrid capsule, enzyme immobilization, hydrophobicity

中图分类号: 

  • TQ658