ICP-MS法测定人源化胶原蛋白中9种微量元素

doi:10.3969/j.issn.1001-1803.2021.10.015

摘要/Abstract

摘要：

建立同时定量测定人源化胶原蛋白中Cr,Fe,Ni,Cu,As,Mo,Cd,Hg和Pb 9种微量元素的方法。对同时测定各元素的线性范围进行考察,并对方法学进行了验证。结果表明,9种微量元素在线性范围内线性良好,相关系数大于0.99,检出浓度为0.1~4.1 ng/kg,定量浓度为0.5~135 ng/kg,精密度RSD为1.8%~6.0%,回收率为88.5%~107.9%。本方法具有高效、简便、准确等优点,可为人源化胶原蛋白生产过程中的质量监测提供方法参考。

关键词: 人源化胶原蛋白, ICP-MS, 微量元素

Abstract:

A method for simultaneous quantitative determination of Cr, Fe, Ni, Cu, As, Mo, Cd, Hg and Pb in human collagen was established. Also, the linear range of each element was investigated. Moreover, this method of quantitative determination was verified. The results show that the nine trace elements present good linearity in the linear range, the correlation coefficient is more than 0.99, the limit of quantitation is 0.1-4.1 ng/kg, the quantitative concentration is 0.5-135 ng/kg, the precision RSD is 1.8%-6.0%, and the recovery is 88.5%-107.9%. This method has advantages of high efficiency, simplicity and accuracy, which can provide a reference for the quality monitoring in the production process of human collagen.

Key words: human collagen, ICP-MS, trace elements

中图分类号:

TQ658

王慧,董庆斌,杨丽萍,代林涛. ICP-MS法测定人源化胶原蛋白中9种微量元素[J]. 日用化学工业, 2021, 51(10): 1026-1031.

Wang Hui,Dong Qingbin,Yang Liping,Dai Lintao. Simultaneous quantitative determination of the nine trace elements in human collagen by ICP-MS[J]. China Surfactant Detergent & Cosmetics, 2021, 51(10): 1026-1031.

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参考文献 12

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元素	回归方程	相关系数R²
Cr	CPS=66 500ρ+32 000	0.998 2
Fe	CPS=2 370ρ+53 800	0.998 1
Ni	CPS=1.560 0ρ+2.980	0.997 4
Cu	CPS=3.580 0ρ+15 300	0.997 7
As	CPS=8 350ρ+9 150	0.997 3
Mo	CPS=19 700ρ+329	0.997 7
Cd	CPS=14 100ρ+166	0.998 1
Hg	CPS=9 740ρ+13.6	0.994 9
Pb	CPS=101 000ρ+12 500	0.998 0

浓度	元素	强度-1	强度-2	强度-3	强度-4	强度-5	强度-6	平均值	RSD/%
低浓度	Cr	298 610	300 138	306 880	302 886	298 257	258 580	294 225	6.00
	Fe	280 451	285 724	288 138	286 590	283 374	270 182	282 410	2.30
	Ni	66 513	67 024	68 102	67 377	66 740	63 358	66 519	2.50
	Cu	157 370	158 017	158 428	157 992	156 026	147 959	155 965	2.60
	As	41 197	42 101	41 867	41 509	41 386	39 513	41 262	2.20
	Mo	75 758	76 094	76 992	76 053	76 574	71 484	75 493	2.70
	Cd	52 643	54 590	55 528	53 327	53 052	49 446	53 098	3.90
	Hg	2 597	2 607	2 611	2 696	2 499	2 519	2 588	2.70
	Pb	393 154	397 175	406 215	400 950	389 632	363 095	391 704	3.90
高浓度	Cr	1 297 600	1 302 217	1 346 599	1 298 110	1 296 251	1 342 812	1 313 932	1.80
	Fe	1 155 615	1 167 751	1 221 682	1 161 914	1 166 439	1 213 348	1 181 125	2.40
	Ni	300 357	300 250	313 582	299 596	298 571	312 632	304 165	2.30
	Cu	683 720	690 331	715 827	684 915	683 665	713 571	695 338	2.20
	As	164 355	167 609	172 339	165 768	165 910	173 433	168 236	2.20
	Mo	362 564	368 921	383 694	363 423	364 526	383 366	371 082	2.70
	Cd	257 022	260 974	272 936	260 186	261 079	274 050	264 375	2.70
	Hg	8 356	8 614	8 657	8 335	8 245	8 491	8 450	1.90
	Pb	1 838 033	1 895 344	1 997 053	1 847 245	1 874 275	1 980 060	1 905 335	3.60

元素	样品名称	本底值/（ng·kg^-1）	测得量/（ng·kg^-1）	实测加入量/μg	加入量/μg	回收率/%	均值/%	RSD/%
Cr	回收率-1	—	200.3	0.192 9	0.2	96.45	94.5	2.2
	回收率-2	—	192.5	0.185 0	0.2	92.50	94.5	2.2
	回收率--1	—	878.1	0.904 1	1.0	90.41	90.5	0.2
	回收率--2	—	902.8	0.905 9	1.0	90.59	90.5	0.2
Fe	回收率-1	—	5 301	5.104	5.0	102.07	103.8	1.7
	回收率-2	—	5 487	5.273	5.0	105.47	103.8	1.7
	回收率--1	—	21 910	22.56	25.0	90.24	90.0	0.4
	回收率--2	—	22 360	22.44	25.0	89.75	90.0	0.4
Ni	回收率-1	4.1	196.4	0.185 1	0.2	92.55	90.9	1.9
	回收率-2	4.1	189.8	0.178 5	0.2	89.25	90.9	1.9
	回收率--1	4.1	866.3	0.887 7	1.0	88.77	89.6	1.0
	回收率--2	4.1	904.7	0.903 8	1.0	90.38	89.6	1.0
Cu	回收率-1	—	191.1	0.184 0	0.2	92.00	90.5	1.8
	回收率-2	—	185.0	0.177 8	0.2	88.90	90.5	1.8
	回收率--1	—	860.6	0.886 1	1.0	88.61	89.0	0.6
	回收率--2	—	891.2	0.894 3	1.0	89.43	89.0	0.6
As	回收率-1	—	194.4	0.187 2	0.2	93.60	91.7	2.1
	回收率-2	—	187.0	0.179 7	0.2	89.85	91.7	2.1
	回收率--1	—	866.0	0.891 6	1.0	89.16	89.0	0.2
	回收率--2	—	886.1	0.889 2	1.0	88.92	89.0	0.2
Mo	回收率-1	—	218.4	0.210 2	0.2	105.10	103.1	2.0
	回收率-2	—	210.4	0.202 2	0.2	101.10	103.1	2.0
	回收率--1	—	988.2	1.017	1.0	101.74	100.5	1.3
	回收率--2	—	990.0	0.993 4	1.0	99.34	100.5	1.3
Cd	回收率-1	—	216.3	0.208 3	0.2	104.15	102.9	1.4
	回收率-2	—	211.4	0.203 1	0.2	101.55	102.9	1.4
	回收率--1	—	994.3	1.023	1.0	102.38	100.4	2.1
	回收率--2	—	980.2	0.983 6	1.0	98.36	100.4	2.1
Hg	回收率-1	2.1	114	0.008 9	0.01	89.00	88.5	0.7
	回收率-2	2.1	113	0.008 8	0.01	88.00	88.5	0.7
	回收率--1	2.1	485	0.047 7	0.1	95.40	96.0	0.7
	回收率--2	2.1	502	0.048 3	0.1	96.60	96.0	0.7
Pb	回收率-1	—	227.7	0.219 2	0.2	109.60	107.9	1.7
	回收率-2	—	220.8	0.212 2	0.2	106.10	107.9	1.7
	回收率--1	—	1 014	1.044	1.0	104.41	104.3	0.2
	回收率--2	—	1 038	1.042	1.0	104.20	104.3	0.2

浓度	元素	强度值（0 min）	强度值（50 min）	强度值（80 min）	强度值（120 min）	平均值	RSD/%
低浓度	Cr	232 468	219 885	236 772	240 284	232 352.25	3.8
	Fe	237 038	228 256	244 232	247 104	239 157.5	3.5
	Ni	49 183	47 157	50 634	51 171	49 536.25	3.6
	Cu	125 317	116 979	124 894	125 686	123 219	3.4
	As	33 061	31 543	33 342	33 735	32 920.25	2.9
	Mo	59 112	58 079	62 333	61 868	60 348	3.4
	Cd	43 132	42 790	45 362	45 993	44 319.25	3.6
	Hg	1 762	2 006	1 978	1 922	1 917	5.7
	Pb	404 332	400 448	436 884	433 999	418 915.75	4.6
高浓度	Cr	1 033 614	953 366	1 040 613	1 014 410	1 010 500.75	3.9
	Fe	925 892	860 684	927 927	902 335	904 209.5	3.5
	Ni	237 453	218 290	236 135	229 221	230 274.75	3.8
	Cu	539 333	493 510	538 993	525 567	524 350.75	4.1
	As	135 548	126 415	137 593	133 382	133 234.5	3.6
	Mo	301 082	281 594	302 034	294 995	294 926.25	3.2
	Cd	218 188	205 912	222 276	213 431	214 951.75	3.3
	Hg	9 204	9 161	9 000	8 654	9 004.75	2.8
	Pb	1 791 303	1 713 083	1 816 236	1 758 661	1 769 820.75	2.5