利用3D皮肤模型研究不同分子量透明质酸的抗刺激作用

doi:10.13218/j.cnki.csdc.2016.06.008

摘要/Abstract

摘要： 将十二烷基硫酸钠(SDS)和不同分子量的透明质酸(HA)共同作用于人永生化表皮细胞(HaCaT)和3D皮肤模型,作用24 h后通过MTT比色法检测细胞活性,利用ELISA方法检测培养液中人白细胞介素(IL-1α)的水平,并进行组织学观察细胞形态。研究在不同实验模型中,不同分子量HA影响SDS对细胞的损伤和释放IL-1α的差异。结果表明,3D皮肤模型可用于HA的体外抗皮肤刺激作用的研究,实验结果更接近人体实际使用情况以及能提供更多的机制信息。

关键词: 十二烷基硫酸钠, 3D皮肤模型, 角质细胞, 透明质酸, 刺激性, 细胞因子

Abstract: Sodium dodecyl sulfate (SDS) mixed with hyaluronic acid (HA) of separate molecular weight were applied on HaCaT cells and 3D skin model separately.After action of 24 h,the cell viability was detected by MTT assay and the level of IL-1α by ELISA method.The difference of exogenous effect of HA to reduce irritation induced by SDS on cell viability and IL-1α releasing between the two in vitro test models was studied.In conclusion,3D skin model can be used to test the anti-irritation effect of HA in vitro.Meanwhile,the exposure and testing results of 3D skin model are more close to human situation and can provide further mechanism information as comparing with the monolayer cell.

Key words: sodium dodecyl sulfate, 3D skin model, keratinocyte, hyaluronic acid, irritation, cytokines

中图分类号:

TQ423.11

喻欢,陈彧,程树军,秦瑶. 利用3D皮肤模型研究不同分子量透明质酸的抗刺激作用[J]. 日用化学工业, 2016, 46(6): 339-343.

YU Huan,CHEN Yu,CHENG Shu-jun,QIN Yao. Study of anti-irritation effect of hyaluronic acid using 3D skin model[J]. China Surfactant Detergent & Cosmetics, 2016, 46(6): 339-343.

参考文献

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