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日用化学工业 ›› 2016, Vol. 46 ›› Issue (6): 334-338.doi: 10.13218/j.cnki.csdc.2016.06.007    

• 开发与应用 • 上一篇    下一篇

十二烷基硫酸钠对角质细胞基因表达的影响及作用通路研究

赵丹1,苏宁2,张佳婵1,李萌1,霍彤1,王昌涛1   

  1. 1.北京工商大学 理学院 北京市植物资源研究开发重点实验室,北京 100048;
    2.中国检验检疫科学研究院,北京 100176
  • 发布日期:2016-06-22
  • 通讯作者: 王昌涛,教授,博士,电话:18910839617,E-mail:wangct@th.btbu.edu.cn。
  • 作者简介:赵 丹(1988-),女,北京人,助理实验师,硕士,电话:15810337559,E-mail:zhao_dan@btbu.edu.cn。
  • 基金资助:
    中国检验检疫科学研究院基本科研业务费装箱资金资助项目(2015JK015);质检公益性行业科研专项(201310132,201410019)

Study with respect to effect of sodium dodecyl sulfate on gene expression of keratinocytes and its signaling pathway

ZHAO Dan1,SU Ning2,ZHANG Jia-chan1,LI Meng1,HUO Tong1,WANG Chang-tao1   

  1. 1.Beijing Key Laboratory of Plant Resources Research and Development,School of Science,Beijing Technology and Business University,Beijing 100048,China;
    2.Chinese Academy of Inspection and Quarantine,Beijing 100176,China
  • Published:2016-06-22

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摘要： 利用人全基因表达谱芯片对十二烷基硫酸钠(SDS)刺激后的角质细胞总RNA进行检测,研究SDS刺激对角质细胞基因表达的影响及作用通路,并对可能引发的疾病进行预测。SDS刺激引起角质细胞中605个基因表达显著上调,368个基因显著下调。主要通过TNF信号通路进行信号传导,经分析筛选出10个关键差异表达基因,包括IL-6,TNF,CCL5,CCL20,CXCL8,CXCL3,CSF2,CSF1,TNFAIP3和NLRC4,以上基因表达异常会促进炎症的发展并激活免疫系统。SDS刺激可能引发特应性皮炎并对过敏与自身免疫疾病有促进作用。

关键词: 十二烷基硫酸钠, 角质细胞, 刺激, 基因芯片, 炎症

Abstract: Detection of total RNA of keratinocytes after irritation of sodium dodecyl sulfate (SDS) was conducted utilizing human mRNA micro array to study the effect of SDS on gene expression of keratinocytes and its signaling pathway,as well as to predict the possibility of inducing disease by the SDS.Results showed that expression of 605 genes are significantly up regulated and 368 genes are significantly down regulated after exposing to SDS.The signals are transformed mainly by TNF signaling pathway.Ten critical genes (IL-6,TNF,CCL5,CCL20,CXCL8,CXCL3,CSF2,CSF1,TNFAIP3 and NLRC4) are screened by the pathways.Abnormal expression of these genes will affect the development of inflammation and activation of immunity.Possibly,irritation of SDS may cause atopic dermatitis as well as promotion of allergies and autoimmune disease.

Key words: sodium dodecyl sulfate, keratinocyte, irritation, micro array, inflammation

中图分类号: 

  • TQ423.11